Molecular biological research and molecular homologous modeling of Bw.03 subgroup
[Objective] To study the molecular biological mechanism for a case of ABO blood group B subtype, and perform three-dimensional modeling of the mutant enzyme. [Methods] The ABO phenotype was identified by the tube method and microcolumn gel method; the ABO gene of the proband was detected by sequence...
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Institute of Blood Transfusion of Chinese Academy of Medical Sciences
2025-01-01
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| Series: | Zhongguo shuxue zazhi |
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| Online Access: | https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.01.019&lang=en |
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| author | WANG Li KONG Yongkui JIN Huifang LIU Xin XIE Ying LIU Xue CHANG Yanli WANG Yafang YANG Shumiao ZHU Di YANG Qiankun |
| author_facet | WANG Li KONG Yongkui JIN Huifang LIU Xin XIE Ying LIU Xue CHANG Yanli WANG Yafang YANG Shumiao ZHU Di YANG Qiankun |
| author_sort | WANG Li |
| collection | DOAJ |
| description | [Objective] To study the molecular biological mechanism for a case of ABO blood group B subtype, and perform three-dimensional modeling of the mutant enzyme. [Methods] The ABO phenotype was identified by the tube method and microcolumn gel method; the ABO gene of the proband was detected by sequence-specific primer polymerase chain reaction (PCR-SSP), and the exon 6 and 7 of the ABO gene were sequenced and analyzed. Homologous modeling of Bw.03 glycosyltransferase (GT) was carried out by Modeller and analyzed by PyMOL2.5.0 software. [Results] The weakening B antigen was detected in the proband sample by forward typing, and anti-B antibody was detected by reverse typing. PCR-SSP detection showed B, O gene, and the sequencing results showed c.721 C>T mutation in exon 7 of the B gene, resulting in p. Arg 241 Trp. Compared with the wild type, the structure of Bw.03GT was partially changed, and the intermolecular force analysis showed that the original three hydrogen bonds at 241 position disappeared. [Conclusion] Blood group molecular biology examination is helpful for the accurate identification of ambiguous blood group. Homologous modeling more intuitively shows the key site for the weakening of Bw.03 GT activity. The intermolecular force analysis can explain the root cause of enzyme activity weakening. |
| format | Article |
| id | doaj-art-b5e9081253c14e04a276570ad32ea144 |
| institution | Kabale University |
| issn | 1004-549X |
| language | zho |
| publishDate | 2025-01-01 |
| publisher | Institute of Blood Transfusion of Chinese Academy of Medical Sciences |
| record_format | Article |
| series | Zhongguo shuxue zazhi |
| spelling | doaj-art-b5e9081253c14e04a276570ad32ea1442025-01-27T01:46:12ZzhoInstitute of Blood Transfusion of Chinese Academy of Medical SciencesZhongguo shuxue zazhi1004-549X2025-01-0138111211510.13303/j.cjbt.issn.1004-549x.2025.01.0191004-549X(2025)1-0112-04Molecular biological research and molecular homologous modeling of Bw.03 subgroupWANG Li0KONG Yongkui1JIN Huifang2LIU Xin3XIE Ying4LIU Xue5CHANG Yanli6WANG Yafang7YANG Shumiao8ZHU Di9YANG Qiankun10Department of Blood Transfusion, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Blood Transfusion, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Blood Transfusion, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Blood Transfusion, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Blood Transfusion, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Blood Transfusion, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Blood Transfusion, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Blood Transfusion, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Blood Transfusion, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Blood Transfusion, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Blood Transfusion, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China[Objective] To study the molecular biological mechanism for a case of ABO blood group B subtype, and perform three-dimensional modeling of the mutant enzyme. [Methods] The ABO phenotype was identified by the tube method and microcolumn gel method; the ABO gene of the proband was detected by sequence-specific primer polymerase chain reaction (PCR-SSP), and the exon 6 and 7 of the ABO gene were sequenced and analyzed. Homologous modeling of Bw.03 glycosyltransferase (GT) was carried out by Modeller and analyzed by PyMOL2.5.0 software. [Results] The weakening B antigen was detected in the proband sample by forward typing, and anti-B antibody was detected by reverse typing. PCR-SSP detection showed B, O gene, and the sequencing results showed c.721 C>T mutation in exon 7 of the B gene, resulting in p. Arg 241 Trp. Compared with the wild type, the structure of Bw.03GT was partially changed, and the intermolecular force analysis showed that the original three hydrogen bonds at 241 position disappeared. [Conclusion] Blood group molecular biology examination is helpful for the accurate identification of ambiguous blood group. Homologous modeling more intuitively shows the key site for the weakening of Bw.03 GT activity. The intermolecular force analysis can explain the root cause of enzyme activity weakening.https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.01.019&lang=enbw blood groupmolecular biologyhomologous modelingintermolecular force analysis |
| spellingShingle | WANG Li KONG Yongkui JIN Huifang LIU Xin XIE Ying LIU Xue CHANG Yanli WANG Yafang YANG Shumiao ZHU Di YANG Qiankun Molecular biological research and molecular homologous modeling of Bw.03 subgroup Zhongguo shuxue zazhi bw blood group molecular biology homologous modeling intermolecular force analysis |
| title | Molecular biological research and molecular homologous modeling of Bw.03 subgroup |
| title_full | Molecular biological research and molecular homologous modeling of Bw.03 subgroup |
| title_fullStr | Molecular biological research and molecular homologous modeling of Bw.03 subgroup |
| title_full_unstemmed | Molecular biological research and molecular homologous modeling of Bw.03 subgroup |
| title_short | Molecular biological research and molecular homologous modeling of Bw.03 subgroup |
| title_sort | molecular biological research and molecular homologous modeling of bw 03 subgroup |
| topic | bw blood group molecular biology homologous modeling intermolecular force analysis |
| url | https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.01.019&lang=en |
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