Antibacterial and Cytotoxic Effects of Moringa oleifera (Moringa) and Azadirachta indica (Neem) Methanolic Extracts against Strains of Enterococcus faecalis
Objective. To evaluate antibacterial and cytotoxic effect of 2 methanolic extracts of Azadirachta indica and Moringa oleifera against strains of Enterococcus faecalis (ATCC 29212) in vitro. Methods. The methanolic extracts of Azadirachta indica and Moringa oleifera were prepared in vitro. The antiba...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2018/1071676 |
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| author | Lucía Arévalo-Híjar Miguel Ángel Aguilar-Luis Stefany Caballero-García Néstor Gonzáles-Soto Juana Del Valle-Mendoza |
| author_facet | Lucía Arévalo-Híjar Miguel Ángel Aguilar-Luis Stefany Caballero-García Néstor Gonzáles-Soto Juana Del Valle-Mendoza |
| author_sort | Lucía Arévalo-Híjar |
| collection | DOAJ |
| description | Objective. To evaluate antibacterial and cytotoxic effect of 2 methanolic extracts of Azadirachta indica and Moringa oleifera against strains of Enterococcus faecalis (ATCC 29212) in vitro. Methods. The methanolic extracts of Azadirachta indica and Moringa oleifera were prepared in vitro. The antibacterial effect of the extracts against Enterococcus faecalis was evaluated using the agar diffusion technique. The minimum inhibitory concentration (MIC) was determined using the microdilution method and the cytotoxicity using the cellular line MDCK. Results. The methanolic extract with the most antibacterial effect during the first 24 and 48 hours against Enterococcus faecalis was Moringa oleifera, evidencing a growth inhibition zone of 35.5 ± 1.05 and 44.83 ± 0.98, respectively. The MIC for both extracts was 75 µg/ml. The bactericidal effect of the Azadirachta indica extract was found at a concentration of 25 µg/ml and a concentration of 75 µg/ml for Moringa extract. Conclusions. In conclusion, we demonstrated that the methanolic extract of Azadirachta indica and Moringa oleifera both have an antibacterial effect against Enterococcus faecalis strains during the first 24 and 48 hours. None of the extracts exhibited toxicity against the cell lines under low concentrations. |
| format | Article |
| id | doaj-art-b5e0b006bd324437b3549bd860e4de62 |
| institution | Kabale University |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-b5e0b006bd324437b3549bd860e4de622025-02-03T06:10:52ZengWileyInternational Journal of Dentistry1687-87281687-87362018-01-01201810.1155/2018/10716761071676Antibacterial and Cytotoxic Effects of Moringa oleifera (Moringa) and Azadirachta indica (Neem) Methanolic Extracts against Strains of Enterococcus faecalisLucía Arévalo-Híjar0Miguel Ángel Aguilar-Luis1Stefany Caballero-García2Néstor Gonzáles-Soto3Juana Del Valle-Mendoza4School of Odontology, Health Sciences Faculty, Universidad Peruana de Ciencias Aplicadas-UPC, Lima, PeruResearch Center and Innovation of the Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Av. San Marcos cdra 2, Cedros de Villa, Lima, PeruSchool of Odontology, Health Sciences Faculty, Universidad Peruana de Ciencias Aplicadas-UPC, Lima, PeruSchool of Odontology, Health Sciences Faculty, Universidad Peruana de Ciencias Aplicadas-UPC, Lima, PeruResearch Center and Innovation of the Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Av. San Marcos cdra 2, Cedros de Villa, Lima, PeruObjective. To evaluate antibacterial and cytotoxic effect of 2 methanolic extracts of Azadirachta indica and Moringa oleifera against strains of Enterococcus faecalis (ATCC 29212) in vitro. Methods. The methanolic extracts of Azadirachta indica and Moringa oleifera were prepared in vitro. The antibacterial effect of the extracts against Enterococcus faecalis was evaluated using the agar diffusion technique. The minimum inhibitory concentration (MIC) was determined using the microdilution method and the cytotoxicity using the cellular line MDCK. Results. The methanolic extract with the most antibacterial effect during the first 24 and 48 hours against Enterococcus faecalis was Moringa oleifera, evidencing a growth inhibition zone of 35.5 ± 1.05 and 44.83 ± 0.98, respectively. The MIC for both extracts was 75 µg/ml. The bactericidal effect of the Azadirachta indica extract was found at a concentration of 25 µg/ml and a concentration of 75 µg/ml for Moringa extract. Conclusions. In conclusion, we demonstrated that the methanolic extract of Azadirachta indica and Moringa oleifera both have an antibacterial effect against Enterococcus faecalis strains during the first 24 and 48 hours. None of the extracts exhibited toxicity against the cell lines under low concentrations.http://dx.doi.org/10.1155/2018/1071676 |
| spellingShingle | Lucía Arévalo-Híjar Miguel Ángel Aguilar-Luis Stefany Caballero-García Néstor Gonzáles-Soto Juana Del Valle-Mendoza Antibacterial and Cytotoxic Effects of Moringa oleifera (Moringa) and Azadirachta indica (Neem) Methanolic Extracts against Strains of Enterococcus faecalis International Journal of Dentistry |
| title | Antibacterial and Cytotoxic Effects of Moringa oleifera (Moringa) and Azadirachta indica (Neem) Methanolic Extracts against Strains of Enterococcus faecalis |
| title_full | Antibacterial and Cytotoxic Effects of Moringa oleifera (Moringa) and Azadirachta indica (Neem) Methanolic Extracts against Strains of Enterococcus faecalis |
| title_fullStr | Antibacterial and Cytotoxic Effects of Moringa oleifera (Moringa) and Azadirachta indica (Neem) Methanolic Extracts against Strains of Enterococcus faecalis |
| title_full_unstemmed | Antibacterial and Cytotoxic Effects of Moringa oleifera (Moringa) and Azadirachta indica (Neem) Methanolic Extracts against Strains of Enterococcus faecalis |
| title_short | Antibacterial and Cytotoxic Effects of Moringa oleifera (Moringa) and Azadirachta indica (Neem) Methanolic Extracts against Strains of Enterococcus faecalis |
| title_sort | antibacterial and cytotoxic effects of moringa oleifera moringa and azadirachta indica neem methanolic extracts against strains of enterococcus faecalis |
| url | http://dx.doi.org/10.1155/2018/1071676 |
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