Diversity Analysis of Rhizosphere Microorganisms in <i>Helichrysum arenarium</i> (L.) Moench and Screening of Growth-Promoting Bacteria in Xinjiang, China
Rhizosphere microorganisms effectively exploit nutrient resources within the rhizosphere, while growth-promoting bacteria in this environment play a vital role in regulating soil fertility and enhancing plant health. In this study, we utilized a comprehensive approach that included the isolation, pu...
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| author | Xiaoyan Xin Wei He Junhui Zhou Yong Chen Xin Huang Jinyu Yang Jianjun Xu Suqin Song |
| author_facet | Xiaoyan Xin Wei He Junhui Zhou Yong Chen Xin Huang Jinyu Yang Jianjun Xu Suqin Song |
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| description | Rhizosphere microorganisms effectively exploit nutrient resources within the rhizosphere, while growth-promoting bacteria in this environment play a vital role in regulating soil fertility and enhancing plant health. In this study, we utilized a comprehensive approach that included the isolation, purification, and identification of dominant microorganisms, alongside high-throughput sequencing technology. This methodology was employed to analyze the primary microbial groups and their diversity within the rhizosphere soil of <i>Helichrysum arenarium</i> (L.) Moench in Altay, Xinjiang, China. By isolating bacterial strains from the rhizosphere soil using a dilution coating method, we successfully obtained 43 distinct strains. Subsequently, selective media were employed to screen for growth-promoting characteristics among these isolated strains derived from the rhizosphere soil of <i>H. arenarium</i> (L.) Moench. The results, obtained through high-throughput amplification sequencing, revealed diverse bacterial communities belonging to 35 phyla, 93 orders, 215 families, 324 genera, and 231 species associated with <i>H. arenarium</i> (L.) Moench, as well as fungal communities comprising 14 phyla, 47 orders, 96 families, 204 genera, and 571 species present in the rhizosphere soil. Among these identified communities, Actinobacteriota emerged as the predominant bacterial phylum while Ascomycetes and Mortieromycetes were recognized as the principal fungal phyla found in the rhizospheric soil of <i>H. arenarium</i> (L.) Moench. Analysis of culturable bacteria’s promotion activity within this rhizospheric environment indicated that three strains—S16, S31, and S29—exhibited the highest solubility index for inorganic phosphorus; additionally, the screened strains S7 and S10 demonstrated nitrogen-fixing capabilities. Furthermore, ten strains exhibiting excellent iron-bearing capacities were identified; notably, strain S16 displayed the highest D/d value indicating, its superior iron-bearing capacity. The growth-promoting bacteria were identified as <i>Kocuria rosea</i>, <i>Priestia megaterium</i>, <i>Bacillus mobilis</i>, <i>Bacillus bataviensis</i>, three variants of <i>Bacillus mycoides</i>, <i>Bacillus paramobilis</i>, <i>Bacillus sonorensis</i>, and <i>Alcaligenes faecalis.</i> This study provides a foundational understanding of how microorganisms in the rhizosphere of <i>H. arenarium</i> (L.) Moench influence soil nutrient release and offers valuable insights into enhancing yield and quality cultivation by isolating, screening, and identifying growth-promoting bacteria from rhizosphere soil. |
| format | Article |
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| spelling | doaj-art-b5dd25640b854e0db0072afd6439cb3e2025-08-20T02:33:51ZengMDPI AGMicrobiology Research2036-74812025-04-011658910.3390/microbiolres16050089Diversity Analysis of Rhizosphere Microorganisms in <i>Helichrysum arenarium</i> (L.) Moench and Screening of Growth-Promoting Bacteria in Xinjiang, ChinaXiaoyan Xin0Wei He1Junhui Zhou2Yong Chen3Xin Huang4Jinyu Yang5Jianjun Xu6Suqin Song7Key Laboratory of Integrated Pest Management on Crops in Northwestern Oasis, Ministry of Agriculture and Rural Affairs, National Plant Protection Scientific Observation and Experiment Station of Korla, Xinjiang Key Laboratory of Agricultural Biosafety, Institute of Plant Protection, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, ChinaKey Laboratory of Integrated Pest Management on Crops in Northwestern Oasis, Ministry of Agriculture and Rural Affairs, National Plant Protection Scientific Observation and Experiment Station of Korla, Xinjiang Key Laboratory of Agricultural Biosafety, Institute of Plant Protection, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, ChinaKey Laboratory of Integrated Pest Management on Crops in Northwestern Oasis, Ministry of Agriculture and Rural Affairs, National Plant Protection Scientific Observation and Experiment Station of Korla, Xinjiang Key Laboratory of Agricultural Biosafety, Institute of Plant Protection, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, ChinaUrumqi Comprehensive Test Station, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830000, Xinjiang, ChinaKey Laboratory of Integrated Pest Management on Crops in Northwestern Oasis, Ministry of Agriculture and Rural Affairs, National Plant Protection Scientific Observation and Experiment Station of Korla, Xinjiang Key Laboratory of Agricultural Biosafety, Institute of Plant Protection, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, ChinaInstitute of Agricultural Resources and Environment, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, ChinaKey Laboratory of Integrated Pest Management on Crops in Northwestern Oasis, Ministry of Agriculture and Rural Affairs, National Plant Protection Scientific Observation and Experiment Station of Korla, Xinjiang Key Laboratory of Agricultural Biosafety, Institute of Plant Protection, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, ChinaKey Laboratory of Integrated Pest Management on Crops in Northwestern Oasis, Ministry of Agriculture and Rural Affairs, National Plant Protection Scientific Observation and Experiment Station of Korla, Xinjiang Key Laboratory of Agricultural Biosafety, Institute of Plant Protection, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, ChinaRhizosphere microorganisms effectively exploit nutrient resources within the rhizosphere, while growth-promoting bacteria in this environment play a vital role in regulating soil fertility and enhancing plant health. In this study, we utilized a comprehensive approach that included the isolation, purification, and identification of dominant microorganisms, alongside high-throughput sequencing technology. This methodology was employed to analyze the primary microbial groups and their diversity within the rhizosphere soil of <i>Helichrysum arenarium</i> (L.) Moench in Altay, Xinjiang, China. By isolating bacterial strains from the rhizosphere soil using a dilution coating method, we successfully obtained 43 distinct strains. Subsequently, selective media were employed to screen for growth-promoting characteristics among these isolated strains derived from the rhizosphere soil of <i>H. arenarium</i> (L.) Moench. The results, obtained through high-throughput amplification sequencing, revealed diverse bacterial communities belonging to 35 phyla, 93 orders, 215 families, 324 genera, and 231 species associated with <i>H. arenarium</i> (L.) Moench, as well as fungal communities comprising 14 phyla, 47 orders, 96 families, 204 genera, and 571 species present in the rhizosphere soil. Among these identified communities, Actinobacteriota emerged as the predominant bacterial phylum while Ascomycetes and Mortieromycetes were recognized as the principal fungal phyla found in the rhizospheric soil of <i>H. arenarium</i> (L.) Moench. Analysis of culturable bacteria’s promotion activity within this rhizospheric environment indicated that three strains—S16, S31, and S29—exhibited the highest solubility index for inorganic phosphorus; additionally, the screened strains S7 and S10 demonstrated nitrogen-fixing capabilities. Furthermore, ten strains exhibiting excellent iron-bearing capacities were identified; notably, strain S16 displayed the highest D/d value indicating, its superior iron-bearing capacity. The growth-promoting bacteria were identified as <i>Kocuria rosea</i>, <i>Priestia megaterium</i>, <i>Bacillus mobilis</i>, <i>Bacillus bataviensis</i>, three variants of <i>Bacillus mycoides</i>, <i>Bacillus paramobilis</i>, <i>Bacillus sonorensis</i>, and <i>Alcaligenes faecalis.</i> This study provides a foundational understanding of how microorganisms in the rhizosphere of <i>H. arenarium</i> (L.) Moench influence soil nutrient release and offers valuable insights into enhancing yield and quality cultivation by isolating, screening, and identifying growth-promoting bacteria from rhizosphere soil.https://www.mdpi.com/2036-7481/16/5/89<i>Helichrysum arenarium</i> (L.) Moenchrhizosphere microorganismscommunity structurediversitygrowth-promoting bacteria |
| spellingShingle | Xiaoyan Xin Wei He Junhui Zhou Yong Chen Xin Huang Jinyu Yang Jianjun Xu Suqin Song Diversity Analysis of Rhizosphere Microorganisms in <i>Helichrysum arenarium</i> (L.) Moench and Screening of Growth-Promoting Bacteria in Xinjiang, China Microbiology Research <i>Helichrysum arenarium</i> (L.) Moench rhizosphere microorganisms community structure diversity growth-promoting bacteria |
| title | Diversity Analysis of Rhizosphere Microorganisms in <i>Helichrysum arenarium</i> (L.) Moench and Screening of Growth-Promoting Bacteria in Xinjiang, China |
| title_full | Diversity Analysis of Rhizosphere Microorganisms in <i>Helichrysum arenarium</i> (L.) Moench and Screening of Growth-Promoting Bacteria in Xinjiang, China |
| title_fullStr | Diversity Analysis of Rhizosphere Microorganisms in <i>Helichrysum arenarium</i> (L.) Moench and Screening of Growth-Promoting Bacteria in Xinjiang, China |
| title_full_unstemmed | Diversity Analysis of Rhizosphere Microorganisms in <i>Helichrysum arenarium</i> (L.) Moench and Screening of Growth-Promoting Bacteria in Xinjiang, China |
| title_short | Diversity Analysis of Rhizosphere Microorganisms in <i>Helichrysum arenarium</i> (L.) Moench and Screening of Growth-Promoting Bacteria in Xinjiang, China |
| title_sort | diversity analysis of rhizosphere microorganisms in i helichrysum arenarium i l moench and screening of growth promoting bacteria in xinjiang china |
| topic | <i>Helichrysum arenarium</i> (L.) Moench rhizosphere microorganisms community structure diversity growth-promoting bacteria |
| url | https://www.mdpi.com/2036-7481/16/5/89 |
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