Phenolic compounds and biological activities of grape (Vitis vinifera L.) seeds at different ripening stages: insights from Algerian varieties
ABSTRACT The surge in interest in bioactive plant compounds, particularly from grapes (Vitis vinifera L.), has led to this study on phenolic compounds at different ripening stages. Grapes were collected before veraison, at veraison, and during ripening. Phenolic compounds were extracted using 50% ac...
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Universidade Federal de Minas Gerais
2025-01-01
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| Series: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352025000100108&lng=en&tlng=en |
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| author | F. Kamah A. Basli R. Erenler A. Bouzana C. Bensouici T. Richard F.A. Al-Mekhlafi M.A. Wadaan M.S. Al-Khalifa F. Boulkenafet |
| author_facet | F. Kamah A. Basli R. Erenler A. Bouzana C. Bensouici T. Richard F.A. Al-Mekhlafi M.A. Wadaan M.S. Al-Khalifa F. Boulkenafet |
| author_sort | F. Kamah |
| collection | DOAJ |
| description | ABSTRACT The surge in interest in bioactive plant compounds, particularly from grapes (Vitis vinifera L.), has led to this study on phenolic compounds at different ripening stages. Grapes were collected before veraison, at veraison, and during ripening. Phenolic compounds were extracted using 50% acetone and quantified through spectrophotometric assays for total phenolic, flavonoid, flavanol, condensed tannin, and hydrolysable tannin contents. LCMS/MS identified phenolics. Antioxidant activities were assessed via (DPPH), (ABTS), phenanthroline, reducing power, and silver nanoparticle assays. Additionally, anti-tyrosinase activity and photoprotective effects were evaluated. The results indicated that before veraison (BV) stages yielded the highest concentrations of phenolic compounds. The most abundant compounds at the berry veraison stage were catechin (72645.40, Sabel), gallic acid (1130.75, Red Glob), and luteolin (164.44, Cardinal). Antioxidant activities were consistent with phenolic content trends, with significant tyrosinase enzyme suppression and high SPF factor observed. Principal Component Analysis highlighted strong correlations between phenolic profiles and antioxidant activities. This study is the first to examine how grape ripeness affects phenolic content and biological activities in Algerian grape seeds. It identifies optimal harvest times for maximizing bioactive compounds and supports the use of grape seed extract in sunscreens and antioxidants. |
| format | Article |
| id | doaj-art-b5cc75c677104792bc5c85ba3e263670 |
| institution | Kabale University |
| issn | 1678-4162 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Universidade Federal de Minas Gerais |
| record_format | Article |
| series | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| spelling | doaj-art-b5cc75c677104792bc5c85ba3e2636702025-01-21T07:40:36ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia1678-41622025-01-0177110.1590/1678-4162-13361Phenolic compounds and biological activities of grape (Vitis vinifera L.) seeds at different ripening stages: insights from Algerian varietiesF. Kamahhttps://orcid.org/0009-0001-6476-0557A. Baslihttps://orcid.org/0000-0001-7535-7029R. Erenlerhttps://orcid.org/0000-0002-0505-3190A. Bouzanahttps://orcid.org/0009-0002-1402-6036C. Bensouicihttps://orcid.org/0000-0003-4612-4642T. Richardhttps://orcid.org/0000-0002-5308-8697F.A. Al-Mekhlafihttps://orcid.org/0000-0002-8190-7901M.A. Wadaanhttps://orcid.org/0000-0002-8330-6990M.S. Al-Khalifahttps://orcid.org/0000-0002-8077-6428F. Boulkenafethttps://orcid.org/0000-0002-0676-930XABSTRACT The surge in interest in bioactive plant compounds, particularly from grapes (Vitis vinifera L.), has led to this study on phenolic compounds at different ripening stages. Grapes were collected before veraison, at veraison, and during ripening. Phenolic compounds were extracted using 50% acetone and quantified through spectrophotometric assays for total phenolic, flavonoid, flavanol, condensed tannin, and hydrolysable tannin contents. LCMS/MS identified phenolics. Antioxidant activities were assessed via (DPPH), (ABTS), phenanthroline, reducing power, and silver nanoparticle assays. Additionally, anti-tyrosinase activity and photoprotective effects were evaluated. The results indicated that before veraison (BV) stages yielded the highest concentrations of phenolic compounds. The most abundant compounds at the berry veraison stage were catechin (72645.40, Sabel), gallic acid (1130.75, Red Glob), and luteolin (164.44, Cardinal). Antioxidant activities were consistent with phenolic content trends, with significant tyrosinase enzyme suppression and high SPF factor observed. Principal Component Analysis highlighted strong correlations between phenolic profiles and antioxidant activities. This study is the first to examine how grape ripeness affects phenolic content and biological activities in Algerian grape seeds. It identifies optimal harvest times for maximizing bioactive compounds and supports the use of grape seed extract in sunscreens and antioxidants.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352025000100108&lng=en&tlng=enrepining stagesantioxidantLCMS/MSanti-tyrosinaseSPF factor |
| spellingShingle | F. Kamah A. Basli R. Erenler A. Bouzana C. Bensouici T. Richard F.A. Al-Mekhlafi M.A. Wadaan M.S. Al-Khalifa F. Boulkenafet Phenolic compounds and biological activities of grape (Vitis vinifera L.) seeds at different ripening stages: insights from Algerian varieties Arquivo Brasileiro de Medicina Veterinária e Zootecnia repining stages antioxidant LCMS/MS anti-tyrosinase SPF factor |
| title | Phenolic compounds and biological activities of grape (Vitis vinifera L.) seeds at different ripening stages: insights from Algerian varieties |
| title_full | Phenolic compounds and biological activities of grape (Vitis vinifera L.) seeds at different ripening stages: insights from Algerian varieties |
| title_fullStr | Phenolic compounds and biological activities of grape (Vitis vinifera L.) seeds at different ripening stages: insights from Algerian varieties |
| title_full_unstemmed | Phenolic compounds and biological activities of grape (Vitis vinifera L.) seeds at different ripening stages: insights from Algerian varieties |
| title_short | Phenolic compounds and biological activities of grape (Vitis vinifera L.) seeds at different ripening stages: insights from Algerian varieties |
| title_sort | phenolic compounds and biological activities of grape vitis vinifera l seeds at different ripening stages insights from algerian varieties |
| topic | repining stages antioxidant LCMS/MS anti-tyrosinase SPF factor |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352025000100108&lng=en&tlng=en |
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