Redox status of pregnant women with thrombophilia

Background/Aim. Since the role of oxidative stress in the pathogenesis of thrombophilia in pregnancy has still not been clarified, the aim of the study was to assess the redox status of pregnant women with thrombophilia. Methods. The study involved 120 pregnant women divided into two groups: pregnan...

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Main Authors: Dimitrijević Aleksandra S., Bradić Jovana V., Živković Vladimir, Dimitrijević Aleksandra B., Milojević-Čorbić Mirjana L., Đurić Janko M., Vasiljević Dragan V., Jakovljević Vladimir Lj.
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Language:English
Published: Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade 2022-01-01
Series:Vojnosanitetski Pregled
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Online Access:http://www.doiserbia.nb.rs/img/doi/0042-8450/2022/0042-84502100001D.pdf
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author Dimitrijević Aleksandra S.
Bradić Jovana V.
Živković Vladimir
Dimitrijević Aleksandra B.
Milojević-Čorbić Mirjana L.
Đurić Janko M.
Vasiljević Dragan V.
Jakovljević Vladimir Lj.
author_facet Dimitrijević Aleksandra S.
Bradić Jovana V.
Živković Vladimir
Dimitrijević Aleksandra B.
Milojević-Čorbić Mirjana L.
Đurić Janko M.
Vasiljević Dragan V.
Jakovljević Vladimir Lj.
author_sort Dimitrijević Aleksandra S.
collection DOAJ
description Background/Aim. Since the role of oxidative stress in the pathogenesis of thrombophilia in pregnancy has still not been clarified, the aim of the study was to assess the redox status of pregnant women with thrombophilia. Methods. The study involved 120 pregnant women divided into two groups: pregnant women with thrombophilia (n = 60) and women with normal pregnancy (n = 60). Blood samples for biochemical analysis were collected at the end of the first, second, and third trimester of pregnancy. Concentrations of hydrogen peroxide (H2O2), nitrites (NO2-), and the index of lipid peroxidation measured as thiobarbituric acid reactive substances (TBARS) were measured in plasma. Levels of reduced glutathione (GSH), activities of superoxide dismutase (SOD) and catalase (CAT) were measured in erythrocytes. Results. In women with thrombophilia, NO2- values were increased in the first and third trimester compared to healthy pregnant women (p < 0.05). The higher levels of TBARS and H2O2 were noticed in women with thrombophilia in the first trimester compared to healthy pregnant women (p < 0.05). The values of SOD and CAT were lower in women with thrombophilia in the third and GSH in the first trimester compared to the control group (p < 0.05). Conclusion. Our results suggest an increased generation of prooxidants in thrombophilia at the beginning of gestation, which declines as gestation progresses and reaches similar values as in normal pregnancy at the end of pregnancy. Generally viewed, pregnant women with thrombophilia was associated with impaired antioxidant capacity – activities of SOD and CAT were lower in the third and GSH in the first trimester compared to their values in healthy pregnant women.
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spelling doaj-art-b5c9b4cd28a44f4a97592bc89d8d8b012025-08-20T02:23:20ZengMinistry of Defence of the Republic of Serbia, University of Defence, BelgradeVojnosanitetski Pregled0042-84502406-07202022-01-0179656557010.2298/VSP200908001D0042-84502100001DRedox status of pregnant women with thrombophiliaDimitrijević Aleksandra S.0Bradić Jovana V.1https://orcid.org/0000-0001-5388-6085Živković Vladimir2Dimitrijević Aleksandra B.3https://orcid.org/0000-0003-0102-3354Milojević-Čorbić Mirjana L.4Đurić Janko M.5Vasiljević Dragan V.6https://orcid.org/0000-0003-3841-9002Jakovljević Vladimir Lj.7Institute of Public Health Kragujevac, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Medical Sciences, Department of Pharmacy, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Medical Sciences, Department of Physiology, Kragujevac, SerbiaUniversity Clinical Center Kragujevac, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Medical Sciences, Kragujevac, SerbiaUniversity Clinical Center Kragujevac, Kragujevac, SerbiaInstitute of Public Health Kragujevac, Kragujevac, Serbia + University of Kragujevac, Faculty of Medical Sciences, Department of Hygiene, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Medical Sciences, Department of Physiology, Kragujevac, Serbia + University IM Sechenov, 1st Moscow State Medical, Department of Human Pathology, Moscow, RussiaBackground/Aim. Since the role of oxidative stress in the pathogenesis of thrombophilia in pregnancy has still not been clarified, the aim of the study was to assess the redox status of pregnant women with thrombophilia. Methods. The study involved 120 pregnant women divided into two groups: pregnant women with thrombophilia (n = 60) and women with normal pregnancy (n = 60). Blood samples for biochemical analysis were collected at the end of the first, second, and third trimester of pregnancy. Concentrations of hydrogen peroxide (H2O2), nitrites (NO2-), and the index of lipid peroxidation measured as thiobarbituric acid reactive substances (TBARS) were measured in plasma. Levels of reduced glutathione (GSH), activities of superoxide dismutase (SOD) and catalase (CAT) were measured in erythrocytes. Results. In women with thrombophilia, NO2- values were increased in the first and third trimester compared to healthy pregnant women (p < 0.05). The higher levels of TBARS and H2O2 were noticed in women with thrombophilia in the first trimester compared to healthy pregnant women (p < 0.05). The values of SOD and CAT were lower in women with thrombophilia in the third and GSH in the first trimester compared to the control group (p < 0.05). Conclusion. Our results suggest an increased generation of prooxidants in thrombophilia at the beginning of gestation, which declines as gestation progresses and reaches similar values as in normal pregnancy at the end of pregnancy. Generally viewed, pregnant women with thrombophilia was associated with impaired antioxidant capacity – activities of SOD and CAT were lower in the third and GSH in the first trimester compared to their values in healthy pregnant women.http://www.doiserbia.nb.rs/img/doi/0042-8450/2022/0042-84502100001D.pdfantioxidantsoxidation-reductionpregnancypregnancy trimestersthrombophilia
spellingShingle Dimitrijević Aleksandra S.
Bradić Jovana V.
Živković Vladimir
Dimitrijević Aleksandra B.
Milojević-Čorbić Mirjana L.
Đurić Janko M.
Vasiljević Dragan V.
Jakovljević Vladimir Lj.
Redox status of pregnant women with thrombophilia
Vojnosanitetski Pregled
antioxidants
oxidation-reduction
pregnancy
pregnancy trimesters
thrombophilia
title Redox status of pregnant women with thrombophilia
title_full Redox status of pregnant women with thrombophilia
title_fullStr Redox status of pregnant women with thrombophilia
title_full_unstemmed Redox status of pregnant women with thrombophilia
title_short Redox status of pregnant women with thrombophilia
title_sort redox status of pregnant women with thrombophilia
topic antioxidants
oxidation-reduction
pregnancy
pregnancy trimesters
thrombophilia
url http://www.doiserbia.nb.rs/img/doi/0042-8450/2022/0042-84502100001D.pdf
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