Comprehensive review on chromatographic analysis of flavonoids in fruits
Flavonoids are natural compounds that are abundant in fruits and have many benefits for human health. Their antioxidant, anti-inflammatory, and anticancer effects make these compounds important for health. Chromatographic analytical methods are the most commonly used methods for detecting, separatin...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-05-01
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| Series: | Journal of Chromatography Open |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772391725000076 |
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| author | Ahmet Cetinkaya Seyda Yayla M. Mesud Hurkul Sibel A. Ozkan |
| author_facet | Ahmet Cetinkaya Seyda Yayla M. Mesud Hurkul Sibel A. Ozkan |
| author_sort | Ahmet Cetinkaya |
| collection | DOAJ |
| description | Flavonoids are natural compounds that are abundant in fruits and have many benefits for human health. Their antioxidant, anti-inflammatory, and anticancer effects make these compounds important for health. Chromatographic analytical methods are the most commonly used methods for detecting, separating, and quantifying flavonoids in fruit species. Methods such as high-performance liquid chromatography (HPLC), gas chromatography (GC), thin-layer chromatography (TLC), and liquid chromatography-tandem mass spectrometry (LC-MS) are crucial for the analysis of flavonoids. In recent years, hybrid systems combining MS and ultraviolet (UV) detectors have been developed to increase the sensitivity of these techniques. HPLC-MS techniques are one of the most preferred methods for determining flavonoids. Flavonoid profiles between different fruit species have been compared using this technique. Although the GC method is less widely used, it uses derivatization steps to analyze non-volatile flavonoids. The accuracy and sensitivity of GC may be limited compared to other methods. TLC, a simpler and more economical method, is still preferred for separating flavonoids. However, it is less widely used than HPLC due to its limited quantitative analysis capacity. Recently, there have been technological developments in chromatographic methods of analysis. Ultra-high-performance liquid chromatography (UHPLC) offers shorter analysis times and higher resolution, especially for separating complex flavonoid mixtures. In this review, the analysis of flavonoids using chromatographic methods and a detailed discussion of studies in the literature to determine flavonoids in fruits are discussed. |
| format | Article |
| id | doaj-art-b5c842fc08b3445eb2fa03608c5e57d8 |
| institution | DOAJ |
| issn | 2772-3917 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Chromatography Open |
| spelling | doaj-art-b5c842fc08b3445eb2fa03608c5e57d82025-08-20T03:13:14ZengElsevierJournal of Chromatography Open2772-39172025-05-01710020910.1016/j.jcoa.2025.100209Comprehensive review on chromatographic analysis of flavonoids in fruitsAhmet Cetinkaya0Seyda Yayla1M. Mesud Hurkul2Sibel A. Ozkan3Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, Ankara, Türkiye; Hacettepe University, Faculty of Pharmacy, Department of Analytical Chemistry, Ankara, TürkiyeAnkara University, Faculty of Pharmacy, Department of Pharmaceutical Botany, Ankara, TürkiyeAnkara University, Faculty of Pharmacy, Department of Pharmaceutical Botany, Ankara, TürkiyeAnkara University, Faculty of Pharmacy, Department of Analytical Chemistry, Ankara, Türkiye; Corresponding author.Flavonoids are natural compounds that are abundant in fruits and have many benefits for human health. Their antioxidant, anti-inflammatory, and anticancer effects make these compounds important for health. Chromatographic analytical methods are the most commonly used methods for detecting, separating, and quantifying flavonoids in fruit species. Methods such as high-performance liquid chromatography (HPLC), gas chromatography (GC), thin-layer chromatography (TLC), and liquid chromatography-tandem mass spectrometry (LC-MS) are crucial for the analysis of flavonoids. In recent years, hybrid systems combining MS and ultraviolet (UV) detectors have been developed to increase the sensitivity of these techniques. HPLC-MS techniques are one of the most preferred methods for determining flavonoids. Flavonoid profiles between different fruit species have been compared using this technique. Although the GC method is less widely used, it uses derivatization steps to analyze non-volatile flavonoids. The accuracy and sensitivity of GC may be limited compared to other methods. TLC, a simpler and more economical method, is still preferred for separating flavonoids. However, it is less widely used than HPLC due to its limited quantitative analysis capacity. Recently, there have been technological developments in chromatographic methods of analysis. Ultra-high-performance liquid chromatography (UHPLC) offers shorter analysis times and higher resolution, especially for separating complex flavonoid mixtures. In this review, the analysis of flavonoids using chromatographic methods and a detailed discussion of studies in the literature to determine flavonoids in fruits are discussed.http://www.sciencedirect.com/science/article/pii/S2772391725000076FlavonoidsFruit samplesAnalytical methodsChromatographyPhenolic compounds |
| spellingShingle | Ahmet Cetinkaya Seyda Yayla M. Mesud Hurkul Sibel A. Ozkan Comprehensive review on chromatographic analysis of flavonoids in fruits Journal of Chromatography Open Flavonoids Fruit samples Analytical methods Chromatography Phenolic compounds |
| title | Comprehensive review on chromatographic analysis of flavonoids in fruits |
| title_full | Comprehensive review on chromatographic analysis of flavonoids in fruits |
| title_fullStr | Comprehensive review on chromatographic analysis of flavonoids in fruits |
| title_full_unstemmed | Comprehensive review on chromatographic analysis of flavonoids in fruits |
| title_short | Comprehensive review on chromatographic analysis of flavonoids in fruits |
| title_sort | comprehensive review on chromatographic analysis of flavonoids in fruits |
| topic | Flavonoids Fruit samples Analytical methods Chromatography Phenolic compounds |
| url | http://www.sciencedirect.com/science/article/pii/S2772391725000076 |
| work_keys_str_mv | AT ahmetcetinkaya comprehensivereviewonchromatographicanalysisofflavonoidsinfruits AT seydayayla comprehensivereviewonchromatographicanalysisofflavonoidsinfruits AT mmesudhurkul comprehensivereviewonchromatographicanalysisofflavonoidsinfruits AT sibelaozkan comprehensivereviewonchromatographicanalysisofflavonoidsinfruits |