<i>Nardostachys jatamansi</i> Extract and Nardosinone Exert Neuroprotective Effects by Suppressing Glucose Metabolic Reprogramming and Modulating T Cell Infiltration
Background: <i>Nardostachys jatamansi</i> DC. (Gansong), a widely utilized herb in traditional Chinese medicine, has been historically employed in the management of various neuropsychiatric disorders. Nardosinone (Nar), a sesquiterpenoid compound, has been identified as one of the princi...
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2025-04-01
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| author | Congyan Duan Weifang Lin Mingjie Zhang Bianxia Xue Wangjie Sun Yang Jin Xiaoxu Zhang Hong Guo Qing Yuan Mingyu Yu Qi Liu Naixuan Wang Hong Wang Honghua Wu Shaoxia Wang |
| author_facet | Congyan Duan Weifang Lin Mingjie Zhang Bianxia Xue Wangjie Sun Yang Jin Xiaoxu Zhang Hong Guo Qing Yuan Mingyu Yu Qi Liu Naixuan Wang Hong Wang Honghua Wu Shaoxia Wang |
| author_sort | Congyan Duan |
| collection | DOAJ |
| description | Background: <i>Nardostachys jatamansi</i> DC. (Gansong), a widely utilized herb in traditional Chinese medicine, has been historically employed in the management of various neuropsychiatric disorders. Nardosinone (Nar), a sesquiterpenoid compound, has been identified as one of the principal bioactive constituents of <i>N. jatamansi</i>. This study investigated the effects of ethyl acetate extract (NJ-1A) from <i>N. jatamansi</i> and its active constituent nardosinone on neuroinflammatory mediator release, glucose metabolic reprogramming, and T cell migration using both in vitro and in vivo experimental models. Methods: Lipopolysaccharide(LPS)-induced BV-2 microglial cells and a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine/probenecid (MPTP/p)-induced male C57BL/6N mouse chronic model of Parkinson’s disease were applied. Results: Both NJ-1A and Nar could significantly suppress LPS-induced production of M1 pro-inflammatory factors or markers in microglia and could inhibit the glycolytic process and promote oxidative phosphorylation via the AKT/mTOR signaling pathway. Furthermore, they exhibited the capacity to attenuate chemokine release from activated microglia, consequently reducing T cell migration. In vivo experiments revealed that NJ-1A and Nar effectively inhibited microglial activation, diminished T cell infiltration, and mitigated the loss of tyrosine hydroxylase (TH)-positive dopaminergic neurons in the substantia nigra of MPTP-induced mice. Conclusions: NJ-1A and nardosinone exert neuroprotective effects through the modulation of microglial polarization states, regulation of metabolic reprogramming, and suppression of T cell infiltration. |
| format | Article |
| id | doaj-art-b5c69a6b4bc44e509f2df1e40e2d6a5e |
| institution | OA Journals |
| issn | 2073-4409 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj-art-b5c69a6b4bc44e509f2df1e40e2d6a5e2025-08-20T01:49:50ZengMDPI AGCells2073-44092025-04-0114964410.3390/cells14090644<i>Nardostachys jatamansi</i> Extract and Nardosinone Exert Neuroprotective Effects by Suppressing Glucose Metabolic Reprogramming and Modulating T Cell InfiltrationCongyan Duan0Weifang Lin1Mingjie Zhang2Bianxia Xue3Wangjie Sun4Yang Jin5Xiaoxu Zhang6Hong Guo7Qing Yuan8Mingyu Yu9Qi Liu10Naixuan Wang11Hong Wang12Honghua Wu13Shaoxia Wang14School of Medical Technology, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Jinghai Dist., Tianjin 301617, ChinaSchool of Medical Technology, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Jinghai Dist., Tianjin 301617, ChinaSchool of Medical Technology, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Jinghai Dist., Tianjin 301617, ChinaState Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Jinghai Dist., Tianjin 301617, ChinaSchool of Medical Technology, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Jinghai Dist., Tianjin 301617, ChinaSchool of Medical Technology, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Jinghai Dist., Tianjin 301617, ChinaSchool of Medical Technology, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Jinghai Dist., Tianjin 301617, ChinaState Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Jinghai Dist., Tianjin 301617, ChinaState Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Jinghai Dist., Tianjin 301617, ChinaSchool of Medical Technology, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Jinghai Dist., Tianjin 301617, ChinaSchool of Medical Technology, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Jinghai Dist., Tianjin 301617, ChinaSchool of Medical Technology, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Jinghai Dist., Tianjin 301617, ChinaSchool of Medical Technology, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Jinghai Dist., Tianjin 301617, ChinaState Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Jinghai Dist., Tianjin 301617, ChinaSchool of Medical Technology, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Jinghai Dist., Tianjin 301617, ChinaBackground: <i>Nardostachys jatamansi</i> DC. (Gansong), a widely utilized herb in traditional Chinese medicine, has been historically employed in the management of various neuropsychiatric disorders. Nardosinone (Nar), a sesquiterpenoid compound, has been identified as one of the principal bioactive constituents of <i>N. jatamansi</i>. This study investigated the effects of ethyl acetate extract (NJ-1A) from <i>N. jatamansi</i> and its active constituent nardosinone on neuroinflammatory mediator release, glucose metabolic reprogramming, and T cell migration using both in vitro and in vivo experimental models. Methods: Lipopolysaccharide(LPS)-induced BV-2 microglial cells and a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine/probenecid (MPTP/p)-induced male C57BL/6N mouse chronic model of Parkinson’s disease were applied. Results: Both NJ-1A and Nar could significantly suppress LPS-induced production of M1 pro-inflammatory factors or markers in microglia and could inhibit the glycolytic process and promote oxidative phosphorylation via the AKT/mTOR signaling pathway. Furthermore, they exhibited the capacity to attenuate chemokine release from activated microglia, consequently reducing T cell migration. In vivo experiments revealed that NJ-1A and Nar effectively inhibited microglial activation, diminished T cell infiltration, and mitigated the loss of tyrosine hydroxylase (TH)-positive dopaminergic neurons in the substantia nigra of MPTP-induced mice. Conclusions: NJ-1A and nardosinone exert neuroprotective effects through the modulation of microglial polarization states, regulation of metabolic reprogramming, and suppression of T cell infiltration.https://www.mdpi.com/2073-4409/14/9/644<i>Nardostachys jatamansi</i>nardosinonemicroglianeuroinflammationmetabolic reprogrammingT cell infiltration |
| spellingShingle | Congyan Duan Weifang Lin Mingjie Zhang Bianxia Xue Wangjie Sun Yang Jin Xiaoxu Zhang Hong Guo Qing Yuan Mingyu Yu Qi Liu Naixuan Wang Hong Wang Honghua Wu Shaoxia Wang <i>Nardostachys jatamansi</i> Extract and Nardosinone Exert Neuroprotective Effects by Suppressing Glucose Metabolic Reprogramming and Modulating T Cell Infiltration Cells <i>Nardostachys jatamansi</i> nardosinone microglia neuroinflammation metabolic reprogramming T cell infiltration |
| title | <i>Nardostachys jatamansi</i> Extract and Nardosinone Exert Neuroprotective Effects by Suppressing Glucose Metabolic Reprogramming and Modulating T Cell Infiltration |
| title_full | <i>Nardostachys jatamansi</i> Extract and Nardosinone Exert Neuroprotective Effects by Suppressing Glucose Metabolic Reprogramming and Modulating T Cell Infiltration |
| title_fullStr | <i>Nardostachys jatamansi</i> Extract and Nardosinone Exert Neuroprotective Effects by Suppressing Glucose Metabolic Reprogramming and Modulating T Cell Infiltration |
| title_full_unstemmed | <i>Nardostachys jatamansi</i> Extract and Nardosinone Exert Neuroprotective Effects by Suppressing Glucose Metabolic Reprogramming and Modulating T Cell Infiltration |
| title_short | <i>Nardostachys jatamansi</i> Extract and Nardosinone Exert Neuroprotective Effects by Suppressing Glucose Metabolic Reprogramming and Modulating T Cell Infiltration |
| title_sort | i nardostachys jatamansi i extract and nardosinone exert neuroprotective effects by suppressing glucose metabolic reprogramming and modulating t cell infiltration |
| topic | <i>Nardostachys jatamansi</i> nardosinone microglia neuroinflammation metabolic reprogramming T cell infiltration |
| url | https://www.mdpi.com/2073-4409/14/9/644 |
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