Protocol for in vivo tracking of lipoproteins in developing oocytes of multiple species of Chagas disease vectors using fluorescent probes
Summary: In insects, the circulating lipoproteins vitellogenin and lipophorin function as yolk protein precursors during oogenesis. Here, we present a protocol for tracing vitellogenin and lipophorin pathways during egg development in three species of Chagas disease vectors (Triatominae, Hemiptera:...
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| Language: | English |
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Elsevier
2025-06-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725002461 |
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| author | Leonardo L. Fruttero Jimena Leyria Pedro A. Paglione Lilián E. Canavoso |
| author_facet | Leonardo L. Fruttero Jimena Leyria Pedro A. Paglione Lilián E. Canavoso |
| author_sort | Leonardo L. Fruttero |
| collection | DOAJ |
| description | Summary: In insects, the circulating lipoproteins vitellogenin and lipophorin function as yolk protein precursors during oogenesis. Here, we present a protocol for tracing vitellogenin and lipophorin pathways during egg development in three species of Chagas disease vectors (Triatominae, Hemiptera: Reduviidae). We describe steps for purifying and fluorescent labeling of lipoproteins, their injection into vitellogenic females, and analysis using confocal microscopy. This approach enables the fate of lipoprotein particles and their lipid cargo in ovarian tissues to be tracked at the subcellular level.For complete details on the use and execution of this protocol, refer to Fruttero et al.1 and Leyria et al.2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-b5c399caf96d4d249ff31cc6fdc2eead |
| institution | OA Journals |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-b5c399caf96d4d249ff31cc6fdc2eead2025-08-20T02:26:11ZengElsevierSTAR Protocols2666-16672025-06-016210384010.1016/j.xpro.2025.103840Protocol for in vivo tracking of lipoproteins in developing oocytes of multiple species of Chagas disease vectors using fluorescent probesLeonardo L. Fruttero0Jimena Leyria1Pedro A. Paglione2Lilián E. Canavoso3Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba CP 5000, Argentina; Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Córdoba CP 5000, Argentina; Corresponding authorDepartamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba CP 5000, Argentina; Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Córdoba CP 5000, Argentina; Corresponding authorDepartamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba CP 5000, Argentina; Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Córdoba CP 5000, ArgentinaDepartamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba CP 5000, Argentina; Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Córdoba CP 5000, Argentina; Corresponding authorSummary: In insects, the circulating lipoproteins vitellogenin and lipophorin function as yolk protein precursors during oogenesis. Here, we present a protocol for tracing vitellogenin and lipophorin pathways during egg development in three species of Chagas disease vectors (Triatominae, Hemiptera: Reduviidae). We describe steps for purifying and fluorescent labeling of lipoproteins, their injection into vitellogenic females, and analysis using confocal microscopy. This approach enables the fate of lipoprotein particles and their lipid cargo in ovarian tissues to be tracked at the subcellular level.For complete details on the use and execution of this protocol, refer to Fruttero et al.1 and Leyria et al.2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725002461Cell BiologyMicroscopyProtein BiochemistryProtocols in Entomology |
| spellingShingle | Leonardo L. Fruttero Jimena Leyria Pedro A. Paglione Lilián E. Canavoso Protocol for in vivo tracking of lipoproteins in developing oocytes of multiple species of Chagas disease vectors using fluorescent probes STAR Protocols Cell Biology Microscopy Protein Biochemistry Protocols in Entomology |
| title | Protocol for in vivo tracking of lipoproteins in developing oocytes of multiple species of Chagas disease vectors using fluorescent probes |
| title_full | Protocol for in vivo tracking of lipoproteins in developing oocytes of multiple species of Chagas disease vectors using fluorescent probes |
| title_fullStr | Protocol for in vivo tracking of lipoproteins in developing oocytes of multiple species of Chagas disease vectors using fluorescent probes |
| title_full_unstemmed | Protocol for in vivo tracking of lipoproteins in developing oocytes of multiple species of Chagas disease vectors using fluorescent probes |
| title_short | Protocol for in vivo tracking of lipoproteins in developing oocytes of multiple species of Chagas disease vectors using fluorescent probes |
| title_sort | protocol for in vivo tracking of lipoproteins in developing oocytes of multiple species of chagas disease vectors using fluorescent probes |
| topic | Cell Biology Microscopy Protein Biochemistry Protocols in Entomology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725002461 |
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