Genome-Wide Identification of Alternative Splicing in <i>Botrytis cinerea</i> During Infection Stage of <i>Solanum lycopersicum</i>
Alternative splicing plays a crucial role in enhancing the protein diversity of eukaryotic genomes. However, alternative splicing has not been extensively studied in <i>Botrytis cinerea</i>. In this study, we examined the distribution and regulation of alternative splicing in the filamen...
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MDPI AG
2025-02-01
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| author | Ping Lu Yun Wang Xiaoli Wang Jiaqi Wang Dan Wang Haojie Shi |
| author_facet | Ping Lu Yun Wang Xiaoli Wang Jiaqi Wang Dan Wang Haojie Shi |
| author_sort | Ping Lu |
| collection | DOAJ |
| description | Alternative splicing plays a crucial role in enhancing the protein diversity of eukaryotic genomes. However, alternative splicing has not been extensively studied in <i>Botrytis cinerea</i>. In this study, we examined the distribution and regulation of alternative splicing in the filamentous plant pathogenic fungus <i>B. cinerea</i> through strand-specific RNA sequencing at various stages of infection of <i>Solanum lycopersicum</i>. During infection (pre-penetration and biotrophic stage), most spliceosome genes had upregulated expression levels, indicating that splicing is altered at this stage. A total of 3308 genes underwent alternative splicing in <i>B. cinerea</i>, resulting in 7466 alternative splicing events, most of which were stage-specific. Transcripts generated through alternative splicing typically exhibit lower expression levels, coding potential, and functional domains, which are more prevalent during the hyphal phase compared to the infestation phase. To conclude, our research offers an extensive analysis of the genome-wide alternative splicing in <i>B. cinerea</i> throughout the infection process, acting as a significant resource for further clarifying the pathogenic mechanisms associated with <i>B. cinerea</i>. |
| format | Article |
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| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-02-01 |
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| series | Microorganisms |
| spelling | doaj-art-b5c252f1d0de4f30baf23653c2c04e192025-08-20T03:12:15ZengMDPI AGMicroorganisms2076-26072025-02-0113236010.3390/microorganisms13020360Genome-Wide Identification of Alternative Splicing in <i>Botrytis cinerea</i> During Infection Stage of <i>Solanum lycopersicum</i>Ping Lu0Yun Wang1Xiaoli Wang2Jiaqi Wang3Dan Wang4Haojie Shi5College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, ChinaCollege of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, ChinaJiangsu Provincial Key Construction Laboratory of Probiotics Preparation, Huaiyin Institute of Technology, Huaian 223003, ChinaCollege of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, ChinaState Key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, ChinaCollege of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, ChinaAlternative splicing plays a crucial role in enhancing the protein diversity of eukaryotic genomes. However, alternative splicing has not been extensively studied in <i>Botrytis cinerea</i>. In this study, we examined the distribution and regulation of alternative splicing in the filamentous plant pathogenic fungus <i>B. cinerea</i> through strand-specific RNA sequencing at various stages of infection of <i>Solanum lycopersicum</i>. During infection (pre-penetration and biotrophic stage), most spliceosome genes had upregulated expression levels, indicating that splicing is altered at this stage. A total of 3308 genes underwent alternative splicing in <i>B. cinerea</i>, resulting in 7466 alternative splicing events, most of which were stage-specific. Transcripts generated through alternative splicing typically exhibit lower expression levels, coding potential, and functional domains, which are more prevalent during the hyphal phase compared to the infestation phase. To conclude, our research offers an extensive analysis of the genome-wide alternative splicing in <i>B. cinerea</i> throughout the infection process, acting as a significant resource for further clarifying the pathogenic mechanisms associated with <i>B. cinerea</i>.https://www.mdpi.com/2076-2607/13/2/360<i>Botrytis cinerea</i>RNA-Seqinfectionalternative splicing |
| spellingShingle | Ping Lu Yun Wang Xiaoli Wang Jiaqi Wang Dan Wang Haojie Shi Genome-Wide Identification of Alternative Splicing in <i>Botrytis cinerea</i> During Infection Stage of <i>Solanum lycopersicum</i> Microorganisms <i>Botrytis cinerea</i> RNA-Seq infection alternative splicing |
| title | Genome-Wide Identification of Alternative Splicing in <i>Botrytis cinerea</i> During Infection Stage of <i>Solanum lycopersicum</i> |
| title_full | Genome-Wide Identification of Alternative Splicing in <i>Botrytis cinerea</i> During Infection Stage of <i>Solanum lycopersicum</i> |
| title_fullStr | Genome-Wide Identification of Alternative Splicing in <i>Botrytis cinerea</i> During Infection Stage of <i>Solanum lycopersicum</i> |
| title_full_unstemmed | Genome-Wide Identification of Alternative Splicing in <i>Botrytis cinerea</i> During Infection Stage of <i>Solanum lycopersicum</i> |
| title_short | Genome-Wide Identification of Alternative Splicing in <i>Botrytis cinerea</i> During Infection Stage of <i>Solanum lycopersicum</i> |
| title_sort | genome wide identification of alternative splicing in i botrytis cinerea i during infection stage of i solanum lycopersicum i |
| topic | <i>Botrytis cinerea</i> RNA-Seq infection alternative splicing |
| url | https://www.mdpi.com/2076-2607/13/2/360 |
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