Modulation of antiviral genes by African swine fever isolates of diverse virulence
African swine fever virus (ASFV), the aetiological agent of a devastating swine disease, has developed several strategies to replicate in porcine macrophages, its main target cells. In this work, we investigated the expression of 84 antiviral genes in macrophages infected with the virulent strain 26...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1591709/full |
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| author | Giulia Franzoni Lorena Mura Susanna Zinellu Pedro J. Sanchez-Cordon Miriam Pedrera Silvia Dei Giudici |
| author_facet | Giulia Franzoni Lorena Mura Susanna Zinellu Pedro J. Sanchez-Cordon Miriam Pedrera Silvia Dei Giudici |
| author_sort | Giulia Franzoni |
| collection | DOAJ |
| description | African swine fever virus (ASFV), the aetiological agent of a devastating swine disease, has developed several strategies to replicate in porcine macrophages, its main target cells. In this work, we investigated the expression of 84 antiviral genes in macrophages infected with the virulent strain 26544/OG10 or the attenuated strain NH/P68. Infection with both strains caused an early activation of antiviral defenses, with up-regulation of RNA-sensing molecules and interferon-stimulating genes. However, as viral replication progresses, down-regulation of key inflammatory genes was observed, especially during infection with NH/P68, suggesting an impairment of macrophages' inflammatory response. Data generated provide a better portrait of ASFV immune evasion strategies. |
| format | Article |
| id | doaj-art-b5bc6ab84e084e7e95940f61c795c5aa |
| institution | DOAJ |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-b5bc6ab84e084e7e95940f61c795c5aa2025-08-20T03:19:19ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-06-011210.3389/fvets.2025.15917091591709Modulation of antiviral genes by African swine fever isolates of diverse virulenceGiulia Franzoni0Lorena Mura1Susanna Zinellu2Pedro J. Sanchez-Cordon3Miriam Pedrera4Silvia Dei Giudici5Department of Animal Health, Istituto Zooprofilattico Sperimentale della Sardegna, Sassari, ItalyDepartment of Animal Health, Istituto Zooprofilattico Sperimentale della Sardegna, Sassari, ItalyDepartment of Animal Health, Istituto Zooprofilattico Sperimentale della Sardegna, Sassari, ItalyCentro de Investigacion en Sanidad Animal (CISA), Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA), Consejo Superior de Investigaciones Cientificas (CSIC), Valdeolmos, Madrid, SpainCentro de Investigacion en Sanidad Animal (CISA), Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA), Consejo Superior de Investigaciones Cientificas (CSIC), Valdeolmos, Madrid, SpainDepartment of Animal Health, Istituto Zooprofilattico Sperimentale della Sardegna, Sassari, ItalyAfrican swine fever virus (ASFV), the aetiological agent of a devastating swine disease, has developed several strategies to replicate in porcine macrophages, its main target cells. In this work, we investigated the expression of 84 antiviral genes in macrophages infected with the virulent strain 26544/OG10 or the attenuated strain NH/P68. Infection with both strains caused an early activation of antiviral defenses, with up-regulation of RNA-sensing molecules and interferon-stimulating genes. However, as viral replication progresses, down-regulation of key inflammatory genes was observed, especially during infection with NH/P68, suggesting an impairment of macrophages' inflammatory response. Data generated provide a better portrait of ASFV immune evasion strategies.https://www.frontiersin.org/articles/10.3389/fvets.2025.1591709/fullAfrican swine fever virusmacrophagesinterferon stimulatory genespattern recognition receptorscytokines |
| spellingShingle | Giulia Franzoni Lorena Mura Susanna Zinellu Pedro J. Sanchez-Cordon Miriam Pedrera Silvia Dei Giudici Modulation of antiviral genes by African swine fever isolates of diverse virulence Frontiers in Veterinary Science African swine fever virus macrophages interferon stimulatory genes pattern recognition receptors cytokines |
| title | Modulation of antiviral genes by African swine fever isolates of diverse virulence |
| title_full | Modulation of antiviral genes by African swine fever isolates of diverse virulence |
| title_fullStr | Modulation of antiviral genes by African swine fever isolates of diverse virulence |
| title_full_unstemmed | Modulation of antiviral genes by African swine fever isolates of diverse virulence |
| title_short | Modulation of antiviral genes by African swine fever isolates of diverse virulence |
| title_sort | modulation of antiviral genes by african swine fever isolates of diverse virulence |
| topic | African swine fever virus macrophages interferon stimulatory genes pattern recognition receptors cytokines |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1591709/full |
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