Design of an Effective sgRNA for CRISPR/Cas9 Knock-Ins and Full Mutant Segregation in Polyploid <i>Synechocystis</i> sp. PCC 6803
<i>Synechocystis</i> sp. PCC 6803 is a highly promising organism for the production of diverse recombinant compounds, including biofuels. However, conventional genetic engineering in <i>Synechocystis</i> presents challenges due to its highly polyploid genome, which not only r...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
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| Series: | SynBio |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2674-0583/3/1/3 |
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