Conservation of plant biodiversity by biotechnology methods

Several methods of plant preservation are currently used in the Republic of Kazakhstan: 1) field gene banks in natural growing areas and pomological collections; 2) preservation of seeds at +4°С, –18°С, and –196°С; 3) сold storage of in vitro shoots at +4°С and +10 ± 2°С; 4) cryopreservation of plan...

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Main Authors: N. V. Romadanova, S. V. Kushnarenko
Format: Article
Language:English
Published: N.I. Vavilov All-Russian Institute of Plant Genetic Resources 2023-04-01
Series:Труды по прикладной ботанике, генетике и селекции
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Online Access:https://elpub.vir.nw.ru/jour/article/view/1515
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author N. V. Romadanova
S. V. Kushnarenko
author_facet N. V. Romadanova
S. V. Kushnarenko
author_sort N. V. Romadanova
collection DOAJ
description Several methods of plant preservation are currently used in the Republic of Kazakhstan: 1) field gene banks in natural growing areas and pomological collections; 2) preservation of seeds at +4°С, –18°С, and –196°С; 3) сold storage of in vitro shoots at +4°С and +10 ± 2°С; 4) cryopreservation of plant tissues and organs at –196°C; 5) preservation of plant DNA at –80°C.Ex situ field collections of plants in Kazakhstan are maintained in botanical gardens of the Republic, arboretums, pomological gardens, nurseries, peasant and farm enterprises, and personal plots. The largest collection of fruit and berry plants is located in the Pomological Garden and includes about 4 thousand varieties, more than 40 thousand hybrids and wild forms of various crops.The Republic of Kazakhstan has about 42,000 accessions in the seed collection. The cereal crop collection is mainly concentrated in the Kazakh Research Institute of Agriculture and Plant Growing – 1/3 of the national genetic resources. The main seed collection of vegetable crops and potatoes (14,700 accessions) is held by the Kazakh Research Institute of Potato and Vegetable Growing. Collections are also available at the Institute of Botany and Phytointroduction and in the Institute of Plant Biology and Biotechnology (IPBB), with the storage mode of + 4°C and –18°C. IPBB also uses cryopreservation technology at –196°C for seed storage.The main in vitro collection of Kazakhstan is kept at the IPBB at + 4°С and +10 ± 2°С; it includes more than 140 accessions of various fruit, berry, nut, vegetable, ornamental, and woody crops, grapes, potatoes, etc. A cryogenic collection of seeds, shoot tips, dormant buds, and embryonic axes in liquid nitrogen (–196°C) is found only in IPBB. The collection includes more than 700 accessions.
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spelling doaj-art-b5b21f523ed44e1cbc3727006f474ecd2025-02-03T08:31:15ZengN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesТруды по прикладной ботанике, генетике и селекции2227-88342619-09822023-04-01184123924810.30901/2227-8834-2023-1-239-248694Conservation of plant biodiversity by biotechnology methodsN. V. Romadanova0S. V. Kushnarenko1Institute of Plant Biology and BiotechnologyInstitute of Plant Biology and BiotechnologySeveral methods of plant preservation are currently used in the Republic of Kazakhstan: 1) field gene banks in natural growing areas and pomological collections; 2) preservation of seeds at +4°С, –18°С, and –196°С; 3) сold storage of in vitro shoots at +4°С and +10 ± 2°С; 4) cryopreservation of plant tissues and organs at –196°C; 5) preservation of plant DNA at –80°C.Ex situ field collections of plants in Kazakhstan are maintained in botanical gardens of the Republic, arboretums, pomological gardens, nurseries, peasant and farm enterprises, and personal plots. The largest collection of fruit and berry plants is located in the Pomological Garden and includes about 4 thousand varieties, more than 40 thousand hybrids and wild forms of various crops.The Republic of Kazakhstan has about 42,000 accessions in the seed collection. The cereal crop collection is mainly concentrated in the Kazakh Research Institute of Agriculture and Plant Growing – 1/3 of the national genetic resources. The main seed collection of vegetable crops and potatoes (14,700 accessions) is held by the Kazakh Research Institute of Potato and Vegetable Growing. Collections are also available at the Institute of Botany and Phytointroduction and in the Institute of Plant Biology and Biotechnology (IPBB), with the storage mode of + 4°C and –18°C. IPBB also uses cryopreservation technology at –196°C for seed storage.The main in vitro collection of Kazakhstan is kept at the IPBB at + 4°С and +10 ± 2°С; it includes more than 140 accessions of various fruit, berry, nut, vegetable, ornamental, and woody crops, grapes, potatoes, etc. A cryogenic collection of seeds, shoot tips, dormant buds, and embryonic axes in liquid nitrogen (–196°C) is found only in IPBB. The collection includes more than 700 accessions.https://elpub.vir.nw.ru/jour/article/view/1515plant collectionsseed collections<i>in vitro</i> collectionscryogenic collections
spellingShingle N. V. Romadanova
S. V. Kushnarenko
Conservation of plant biodiversity by biotechnology methods
Труды по прикладной ботанике, генетике и селекции
plant collections
seed collections
<i>in vitro</i> collections
cryogenic collections
title Conservation of plant biodiversity by biotechnology methods
title_full Conservation of plant biodiversity by biotechnology methods
title_fullStr Conservation of plant biodiversity by biotechnology methods
title_full_unstemmed Conservation of plant biodiversity by biotechnology methods
title_short Conservation of plant biodiversity by biotechnology methods
title_sort conservation of plant biodiversity by biotechnology methods
topic plant collections
seed collections
<i>in vitro</i> collections
cryogenic collections
url https://elpub.vir.nw.ru/jour/article/view/1515
work_keys_str_mv AT nvromadanova conservationofplantbiodiversitybybiotechnologymethods
AT svkushnarenko conservationofplantbiodiversitybybiotechnologymethods