Putative Upstream Regulators <i>DoNF-YB3</i> and <i>DoIDD12</i> Correlate with <i>DoGSTF11</i> Expression and Anthocyanin Accumulation in <i>Dendrobium officinale</i>
<i>Dendrobium officinale</i> is a traditional and valuable medicinal herb, with extensive research conducted on its polysaccharides, alkaloids, and other components, yet studies on anthocyanins remain limited. In this study, we analyzed the expression levels of GST family genes in green...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
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| Series: | Horticulturae |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-7524/11/6/711 |
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| Summary: | <i>Dendrobium officinale</i> is a traditional and valuable medicinal herb, with extensive research conducted on its polysaccharides, alkaloids, and other components, yet studies on anthocyanins remain limited. In this study, we analyzed the expression levels of GST family genes in green and purplish <i>D. officinale</i> and found that <i>DoGSTF11</i> is highly expressed in the purplish variety. DoGSTF11 is localized to the nucleus and cell membrane but lacks transcriptional activation activity. Overexpression of <i>DoGSTF11</i> in tomato enhances anthocyanin accumulation, suggesting a role in anthocyanin sequestration or transport. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays further revealed that DoGSTF11 interacts with DoGST31, while <i>DoIDD12</i> and <i>DoNF-YB3</i> are potential transcriptional regulators based on promoter-binding assays and expression correlation. In conclusion, our study demonstrates that <i>DoGST11</i> positively regulates anthocyanin accumulation in <i>D. officinale</i>. These findings provide valuable insights into the metabolic engineering of flavonoids in <i>D. officinale</i>. |
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| ISSN: | 2311-7524 |