Effects of Taohong Siwu Decoction on renal protection and ERK1/2/HIF-1α signaling pathway in rats with mesangial proliferative glomerulonephritis
ObjectiveTo explore the therapeutic effect of Taohong Siwu Decoction （ThSwT） on mesangial proliferative nephritis （MsPGN） in rats and examine its regulatory effect on extracellular regulated protein kinase 1/2 （ERK1/2）/hypoxia inducible factor 1α （HIF-1α） signaling pathway.MethodsA total of 72 Sprag...
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Editorial Department of Journal of Clinical Nephrology
2022-02-01
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| Series: | Linchuang shenzangbing zazhi |
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| Online Access: | http://www.lcszb.com/thesisDetails#10.3969/j.issn.1671-2390.2022.02.009 |
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| author | Wu Xue-bing Ao Hua Li Ming |
| author_facet | Wu Xue-bing Ao Hua Li Ming |
| author_sort | Wu Xue-bing |
| collection | DOAJ |
| description | ObjectiveTo explore the therapeutic effect of Taohong Siwu Decoction （ThSwT） on mesangial proliferative nephritis （MsPGN） in rats and examine its regulatory effect on extracellular regulated protein kinase 1/2 （ERK1/2）/hypoxia inducible factor 1α （HIF-1α） signaling pathway.MethodsA total of 72 Sprague-Dawley （SD） rats were randomly divided into six groups of normal control，model，low-dose （4.5 g/kg），medium-dose （9 g/kg），high-dose （18 g/kg） ThSwT and positive control （5 mg/kg of prednisone acetate） （<italic>n</italic>=12 each）. Except for normal control group，another group received a subcutaneous injection of bovine serum albumin （BSA） for establishing a rat model of MsPGN. ThSwTwas delivered via gavage after successful modeling. Each group was dosed once daily for 28 daysand at 24 h after the last dose. General behavior changes were observed and 24 h urinary protein was detected；the levels of creatinine （Scr） and blood urea nitrogen （BUN） were detected by enzyme-linked immunosorbent assay and the proliferation of glomerular mesangial area was observed by transmission electron microscope；Periodic acid-leuco fuchsin staining method （PAS） for observing the extracellular matrix proliferation of glomerular mesangial cells；the expressions of immunoglobulin M （IgM） and collagen IV （Col-IV） detected by immunofluorescent staining；the expressions of ERK1/2 and HIF-1α by immunohistochemistry；the expressions of ERK1/2，p-ERK1/2，tissue inhibitor of metalloproteinase-1 （TIMP-1），matrix metalloproteinase-9 （MMP-9），tumor necrosis factor-α （TNF-α） and interleukin-6 （IL-6） by Western blot.ResultsCompared with those in normal control group，electronic density deposition of glomerular mesangial area，glomerular epithelial cell foot swelling，glomerular basement membrane thickening and irregular arrangement were serious in model group，24 h urinary protein，Scr，BUN，IgM &Col-IV deposition and positive expressions of ERK1/2，HIF-1α，p-ERK1/2/ERK1/2，MMP-9，TNF-α and IL-6 increased （<italic>P</italic><0.05）. Compared with those in model group，electronic density deposition of glomerular mesangial area，glomerular epithelial cell foot swelling，glomerular basement membrane thickening and irregular arrangement became alleviated in low/medium/high-dose ThSwT and positive control groups，24 h urinary protein，Scr，BUN，IgM &Col-IV deposition，ERK1/2 &HIF-1α positive expression and protein expressions of p-ERK1/2/ERK1/2，MMP-9，TNF-α and IL-6 declined （<italic>P</italic><0.05）. With the incremental dose of ThSwT，the above parameters changed more significantly （<italic>P</italic><0.05） while no significant difference existed between medium-dose ThSwT and positive control groups （<italic>P</italic>>0.05）.ConclusionsThSwT may inhibit the activation of ERK1/2/HIF-1α pathway，retard the process of renal inflammation and fibrosis and improve the process of renal injury in MsPGN rats. |
| format | Article |
| id | doaj-art-b5a6bb809f3646058066708c01f0ef5f |
| institution | OA Journals |
| issn | 1671-2390 |
| language | zho |
| publishDate | 2022-02-01 |
| publisher | Editorial Department of Journal of Clinical Nephrology |
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| series | Linchuang shenzangbing zazhi |
| spelling | doaj-art-b5a6bb809f3646058066708c01f0ef5f2025-08-20T02:16:14ZzhoEditorial Department of Journal of Clinical NephrologyLinchuang shenzangbing zazhi1671-23902022-02-012213914623501716Effects of Taohong Siwu Decoction on renal protection and ERK1/2/HIF-1α signaling pathway in rats with mesangial proliferative glomerulonephritisWu Xue-bingAo HuaLi MingObjectiveTo explore the therapeutic effect of Taohong Siwu Decoction （ThSwT） on mesangial proliferative nephritis （MsPGN） in rats and examine its regulatory effect on extracellular regulated protein kinase 1/2 （ERK1/2）/hypoxia inducible factor 1α （HIF-1α） signaling pathway.MethodsA total of 72 Sprague-Dawley （SD） rats were randomly divided into six groups of normal control，model，low-dose （4.5 g/kg），medium-dose （9 g/kg），high-dose （18 g/kg） ThSwT and positive control （5 mg/kg of prednisone acetate） （<italic>n</italic>=12 each）. Except for normal control group，another group received a subcutaneous injection of bovine serum albumin （BSA） for establishing a rat model of MsPGN. ThSwTwas delivered via gavage after successful modeling. Each group was dosed once daily for 28 daysand at 24 h after the last dose. General behavior changes were observed and 24 h urinary protein was detected；the levels of creatinine （Scr） and blood urea nitrogen （BUN） were detected by enzyme-linked immunosorbent assay and the proliferation of glomerular mesangial area was observed by transmission electron microscope；Periodic acid-leuco fuchsin staining method （PAS） for observing the extracellular matrix proliferation of glomerular mesangial cells；the expressions of immunoglobulin M （IgM） and collagen IV （Col-IV） detected by immunofluorescent staining；the expressions of ERK1/2 and HIF-1α by immunohistochemistry；the expressions of ERK1/2，p-ERK1/2，tissue inhibitor of metalloproteinase-1 （TIMP-1），matrix metalloproteinase-9 （MMP-9），tumor necrosis factor-α （TNF-α） and interleukin-6 （IL-6） by Western blot.ResultsCompared with those in normal control group，electronic density deposition of glomerular mesangial area，glomerular epithelial cell foot swelling，glomerular basement membrane thickening and irregular arrangement were serious in model group，24 h urinary protein，Scr，BUN，IgM &Col-IV deposition and positive expressions of ERK1/2，HIF-1α，p-ERK1/2/ERK1/2，MMP-9，TNF-α and IL-6 increased （<italic>P</italic><0.05）. Compared with those in model group，electronic density deposition of glomerular mesangial area，glomerular epithelial cell foot swelling，glomerular basement membrane thickening and irregular arrangement became alleviated in low/medium/high-dose ThSwT and positive control groups，24 h urinary protein，Scr，BUN，IgM &Col-IV deposition，ERK1/2 &HIF-1α positive expression and protein expressions of p-ERK1/2/ERK1/2，MMP-9，TNF-α and IL-6 declined （<italic>P</italic><0.05）. With the incremental dose of ThSwT，the above parameters changed more significantly （<italic>P</italic><0.05） while no significant difference existed between medium-dose ThSwT and positive control groups （<italic>P</italic>>0.05）.ConclusionsThSwT may inhibit the activation of ERK1/2/HIF-1α pathway，retard the process of renal inflammation and fibrosis and improve the process of renal injury in MsPGN rats.http://www.lcszb.com/thesisDetails#10.3969/j.issn.1671-2390.2022.02.009Taohong Siwu DecoctionGlomerulonephritis，membranoproliferativeMitogen-activated protein kinaseHypoxia-Inducible Factor 1，alpha subunit |
| spellingShingle | Wu Xue-bing Ao Hua Li Ming Effects of Taohong Siwu Decoction on renal protection and ERK1/2/HIF-1α signaling pathway in rats with mesangial proliferative glomerulonephritis Linchuang shenzangbing zazhi Taohong Siwu Decoction Glomerulonephritis，membranoproliferative Mitogen-activated protein kinase Hypoxia-Inducible Factor 1，alpha subunit |
| title | Effects of Taohong Siwu Decoction on renal protection and ERK1/2/HIF-1α signaling pathway in rats with mesangial proliferative glomerulonephritis |
| title_full | Effects of Taohong Siwu Decoction on renal protection and ERK1/2/HIF-1α signaling pathway in rats with mesangial proliferative glomerulonephritis |
| title_fullStr | Effects of Taohong Siwu Decoction on renal protection and ERK1/2/HIF-1α signaling pathway in rats with mesangial proliferative glomerulonephritis |
| title_full_unstemmed | Effects of Taohong Siwu Decoction on renal protection and ERK1/2/HIF-1α signaling pathway in rats with mesangial proliferative glomerulonephritis |
| title_short | Effects of Taohong Siwu Decoction on renal protection and ERK1/2/HIF-1α signaling pathway in rats with mesangial proliferative glomerulonephritis |
| title_sort | effects of taohong siwu decoction on renal protection and erk1 2 hif 1α signaling pathway in rats with mesangial proliferative glomerulonephritis |
| topic | Taohong Siwu Decoction Glomerulonephritis，membranoproliferative Mitogen-activated protein kinase Hypoxia-Inducible Factor 1，alpha subunit |
| url | http://www.lcszb.com/thesisDetails#10.3969/j.issn.1671-2390.2022.02.009 |
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