Low sensitivity of ParaHIT-f rapid malaria test among patients with fever in rural health centers, Northern Tanzania
Introduction: Several rapid diagnostic tools for malaria are currently available in local markets. However, diagnostic accuracy varies widely. The present study was conducted to evaluate a cheaply and easily available rapid diagnostic malaria test (ParaHIT-f) in rural Tanzania. Methodology: Parti...
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The Journal of Infection in Developing Countries
2011-03-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/1346 |
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| author | Eliningaya John Kweka Asanterabi Lowassa Shandala Msangi Epiphania E Kimaro Ester E Lyatuu Beda J Mwang'onde Aneth M Mahande Humphrey D Mazigo |
| author_facet | Eliningaya John Kweka Asanterabi Lowassa Shandala Msangi Epiphania E Kimaro Ester E Lyatuu Beda J Mwang'onde Aneth M Mahande Humphrey D Mazigo |
| author_sort | Eliningaya John Kweka |
| collection | DOAJ |
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Introduction: Several rapid diagnostic tools for malaria are currently available in local markets. However, diagnostic accuracy varies widely. The present study was conducted to evaluate a cheaply and easily available rapid diagnostic malaria test (ParaHIT-f) in rural Tanzania.
Methodology: Participants presenting with fever at health centers in the Kilimanjaro and Manyara regions were eligible. Parasitological thin and thick smears were examined from finger-prick blood samples and compared to ParaHIT-f test results. Sensitivity, specificity and predictive values were calculated using microscopic parasitological examination as the gold standard.
Results: In total, 236/743 (31.8%) individuals had a positive malaria microscopy, and 25/715 (3.4%) were positive in the rapid diagnostic test. The sensitivity of ParaHIT-f was 10.7% (95% CI, 6.7-14.7) and specificity was 100% (95% CI, 97.4-102), with positive and negative predictive values (PPV and NPV) of 100% (95% CI, 99.1-100.2) and 70.9% (95% CI, 66.9-74.9) respectively. Sensitivity of ParaHIT-f increased with increasing P. falciparum density (P > 0.003) from 5.8% (95% CI, 0-12.9) at < 100 parasites/μl to 20.5% (95% CI, 13.5-27) at ≥ 100 parasites/μl.
Conclusions: Sensitivity of the ParaHIT-f rapid test was very low in this setting, therefore concomitant use of rapid diagnostic tests and microscopy is recommended. In the case of positive test results, confirmation by parasitological techniques is not necessary. Further monitoring of ParaHIT-f in various epidemiological settings in Tanzania is warranted.
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| format | Article |
| id | doaj-art-b5a1ffc55a4a48959a0ae76776d148f6 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2011-03-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-b5a1ffc55a4a48959a0ae76776d148f62025-08-20T02:14:10ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802011-03-0150310.3855/jidc.1346Low sensitivity of ParaHIT-f rapid malaria test among patients with fever in rural health centers, Northern TanzaniaEliningaya John Kweka0Asanterabi Lowassa1Shandala Msangi2Epiphania E Kimaro3Ester E Lyatuu4Beda J Mwang'onde5Aneth M Mahande6Humphrey D Mazigo7Tropical Pesticides Research Institute, Division of Livestock and Human Disease Vectors Control, PO Box 3024, Arusha, TanzaniaTanzania Wildlife Research Institute, PO Box 661, Arusha, TanzaniaTropical Pesticides Research Institute, Division of Livestock and Human Disease Vectors Control, PO Box 3024, Arusha, TanzaniaTropical Pesticides Research Institute, Division of Livestock and Human Disease Vectors Control, PO Box 3024, Arusha, TanzaniaTropical Pesticides Research Institute, Division of Livestock and Human Disease Vectors Control, PO Box 3024, Arusha, TanzaniaTropical Pesticides Research Institute, Division of Livestock and Human Disease Vectors Control, PO Box 3024, Arusha, TanzaniaTropical Pesticides Research Institute, Mabogini field station, Moshi, TanzaniaWeill-Bugando University College of Health Sciences, PO Box 1464, Mwanza, Tanzania Introduction: Several rapid diagnostic tools for malaria are currently available in local markets. However, diagnostic accuracy varies widely. The present study was conducted to evaluate a cheaply and easily available rapid diagnostic malaria test (ParaHIT-f) in rural Tanzania. Methodology: Participants presenting with fever at health centers in the Kilimanjaro and Manyara regions were eligible. Parasitological thin and thick smears were examined from finger-prick blood samples and compared to ParaHIT-f test results. Sensitivity, specificity and predictive values were calculated using microscopic parasitological examination as the gold standard. Results: In total, 236/743 (31.8%) individuals had a positive malaria microscopy, and 25/715 (3.4%) were positive in the rapid diagnostic test. The sensitivity of ParaHIT-f was 10.7% (95% CI, 6.7-14.7) and specificity was 100% (95% CI, 97.4-102), with positive and negative predictive values (PPV and NPV) of 100% (95% CI, 99.1-100.2) and 70.9% (95% CI, 66.9-74.9) respectively. Sensitivity of ParaHIT-f increased with increasing P. falciparum density (P > 0.003) from 5.8% (95% CI, 0-12.9) at < 100 parasites/μl to 20.5% (95% CI, 13.5-27) at ≥ 100 parasites/μl. Conclusions: Sensitivity of the ParaHIT-f rapid test was very low in this setting, therefore concomitant use of rapid diagnostic tests and microscopy is recommended. In the case of positive test results, confirmation by parasitological techniques is not necessary. Further monitoring of ParaHIT-f in various epidemiological settings in Tanzania is warranted. https://jidc.org/index.php/journal/article/view/1346RDTPlasmodium falciparummalariaTanzania |
| spellingShingle | Eliningaya John Kweka Asanterabi Lowassa Shandala Msangi Epiphania E Kimaro Ester E Lyatuu Beda J Mwang'onde Aneth M Mahande Humphrey D Mazigo Low sensitivity of ParaHIT-f rapid malaria test among patients with fever in rural health centers, Northern Tanzania Journal of Infection in Developing Countries RDT Plasmodium falciparum malaria Tanzania |
| title | Low sensitivity of ParaHIT-f rapid malaria test among patients with fever in rural health centers, Northern Tanzania |
| title_full | Low sensitivity of ParaHIT-f rapid malaria test among patients with fever in rural health centers, Northern Tanzania |
| title_fullStr | Low sensitivity of ParaHIT-f rapid malaria test among patients with fever in rural health centers, Northern Tanzania |
| title_full_unstemmed | Low sensitivity of ParaHIT-f rapid malaria test among patients with fever in rural health centers, Northern Tanzania |
| title_short | Low sensitivity of ParaHIT-f rapid malaria test among patients with fever in rural health centers, Northern Tanzania |
| title_sort | low sensitivity of parahit f rapid malaria test among patients with fever in rural health centers northern tanzania |
| topic | RDT Plasmodium falciparum malaria Tanzania |
| url | https://jidc.org/index.php/journal/article/view/1346 |
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