Human and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis.
Satellite cells are the chief contributor to skeletal muscle growth and regeneration. The study of mouse satellite cells has accelerated in recent years due to technical advancements in the isolation of these cells. The study of human satellite cells has lagged and thus little is known about how the...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0090398&type=printable |
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| author | Akshay Bareja Jason A Holt Guizhen Luo Calvin Chang Junyu Lin Aaron C Hinken Johannes M Freudenberg William E Kraus William J Evans Andrew N Billin |
| author_facet | Akshay Bareja Jason A Holt Guizhen Luo Calvin Chang Junyu Lin Aaron C Hinken Johannes M Freudenberg William E Kraus William J Evans Andrew N Billin |
| author_sort | Akshay Bareja |
| collection | DOAJ |
| description | Satellite cells are the chief contributor to skeletal muscle growth and regeneration. The study of mouse satellite cells has accelerated in recent years due to technical advancements in the isolation of these cells. The study of human satellite cells has lagged and thus little is known about how the biology of mouse and human satellite cells compare. We developed a flow cytometry-based method to prospectively isolate human skeletal muscle progenitors from the satellite cell pool using positive and negative selection markers. Results show that this pool is enriched in PAX7 expressing cells that possess robust myogenic potential including the ability to give rise to de novo muscle in vivo. We compared mouse and human satellite cells in culture and identify differences in the elaboration of the myogenic genetic program and in the sensitivity of the cells to cytokine stimulation. These results indicate that not all mechanisms regulating mouse satellite cell activation are conserved in human satellite cells and that such differences may impact the clinical translation of therapeutics validated in mouse models. Thus, the findings of this study are relevant to developing therapies to combat muscle disease. |
| format | Article |
| id | doaj-art-b59f032fb88a4b6cac5f3fde409dca6f |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-b59f032fb88a4b6cac5f3fde409dca6f2025-08-20T02:15:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0192e9039810.1371/journal.pone.0090398Human and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis.Akshay BarejaJason A HoltGuizhen LuoCalvin ChangJunyu LinAaron C HinkenJohannes M FreudenbergWilliam E KrausWilliam J EvansAndrew N BillinSatellite cells are the chief contributor to skeletal muscle growth and regeneration. The study of mouse satellite cells has accelerated in recent years due to technical advancements in the isolation of these cells. The study of human satellite cells has lagged and thus little is known about how the biology of mouse and human satellite cells compare. We developed a flow cytometry-based method to prospectively isolate human skeletal muscle progenitors from the satellite cell pool using positive and negative selection markers. Results show that this pool is enriched in PAX7 expressing cells that possess robust myogenic potential including the ability to give rise to de novo muscle in vivo. We compared mouse and human satellite cells in culture and identify differences in the elaboration of the myogenic genetic program and in the sensitivity of the cells to cytokine stimulation. These results indicate that not all mechanisms regulating mouse satellite cell activation are conserved in human satellite cells and that such differences may impact the clinical translation of therapeutics validated in mouse models. Thus, the findings of this study are relevant to developing therapies to combat muscle disease.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0090398&type=printable |
| spellingShingle | Akshay Bareja Jason A Holt Guizhen Luo Calvin Chang Junyu Lin Aaron C Hinken Johannes M Freudenberg William E Kraus William J Evans Andrew N Billin Human and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis. PLoS ONE |
| title | Human and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis. |
| title_full | Human and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis. |
| title_fullStr | Human and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis. |
| title_full_unstemmed | Human and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis. |
| title_short | Human and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis. |
| title_sort | human and mouse skeletal muscle stem cells convergent and divergent mechanisms of myogenesis |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0090398&type=printable |
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