Operational evaluation of the deployment of Malaria/CRP Duo and Dengue Duo rapid diagnostic tests for the management of febrile illness by village malaria workers in rural Cambodia

Abstract Introduction The decline in malaria cases in Cambodia has led to a relative increase in non-malarial febrile illness. In rural Cambodia, village malaria workers (VMWs) provide early diagnosis and treatment for malaria, but their role and relevance are diminishing as malaria cases decline. E...

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Main Authors: Marc T. Visser, Dysoley Lek, Bipin Adhikari, Arjun Chandna, Moul Vanna, Sam Ol, Voeurng Bunreth, Chan Davoeung, Yok Sovann, Dafne Umans, Yoel Lubell, Rusheng Chew, Richard J. Maude, Rob W. van der Pluijm, Rupam Tripura, Naomi Waithira, Siv Sovannaroth, Michèle van Vugt, Lorenz von Seidlein, Mavuto Mukaka, Arjen Dondorp, Thomas J. Peto, James J. Callery
Format: Article
Language:English
Published: BMC 2025-05-01
Series:BMC Infectious Diseases
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Online Access:https://doi.org/10.1186/s12879-025-11016-z
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author Marc T. Visser
Dysoley Lek
Bipin Adhikari
Arjun Chandna
Moul Vanna
Sam Ol
Voeurng Bunreth
Chan Davoeung
Yok Sovann
Dafne Umans
Yoel Lubell
Rusheng Chew
Richard J. Maude
Rob W. van der Pluijm
Rupam Tripura
Naomi Waithira
Siv Sovannaroth
Michèle van Vugt
Lorenz von Seidlein
Mavuto Mukaka
Arjen Dondorp
Thomas J. Peto
James J. Callery
author_facet Marc T. Visser
Dysoley Lek
Bipin Adhikari
Arjun Chandna
Moul Vanna
Sam Ol
Voeurng Bunreth
Chan Davoeung
Yok Sovann
Dafne Umans
Yoel Lubell
Rusheng Chew
Richard J. Maude
Rob W. van der Pluijm
Rupam Tripura
Naomi Waithira
Siv Sovannaroth
Michèle van Vugt
Lorenz von Seidlein
Mavuto Mukaka
Arjen Dondorp
Thomas J. Peto
James J. Callery
author_sort Marc T. Visser
collection DOAJ
description Abstract Introduction The decline in malaria cases in Cambodia has led to a relative increase in non-malarial febrile illness. In rural Cambodia, village malaria workers (VMWs) provide early diagnosis and treatment for malaria, but their role and relevance are diminishing as malaria cases decline. Expanding VMW roles would ensure continued utilisation of their services until malaria elimination is achieved and strengthen community health services. Methods A mixed methods operational research study was implemented to evaluate the use of two combination-RDTs (combo-RDTs) as an expansion of the VMW role, enabling VMWs in Cambodia to test for diseases other than malaria for the first time. VMWs in 78 villages in Battambang and Pailin Provinces were trained and provided with either a Malaria/CRP Duo or Dengue Duo RDT to assess febrile patients. Data were collected on VMW consultations, and combo-RDT usage and results. Focus group discussions (FGDs) and competency assessments of combo-RDT usage were conducted with VMWs. The main objectives were to determine whether VMWs could perform these combo-RDTs correctly and follow management algorithms, and whether deployment had an impact on VMW consultation rates. Perspectives concerning role expansion and the feasibility of conducting additional tests were also explored. Results Between June 2022, and May 2023, a total of 2,425 febrile patients were assessed with either a Malaria/CRP Duo or Dengue Duo RDT. Active dengue infection (NS1- and/or IgM-positive) was identified in 1.2% (11/915) of patients. Positive CRP results (> 20 mg/L) were found for 3.2% (48/1,510) of patients. Following deployment, there was an immediate mean increase of 4.4 VMW consultations per month, from 9.0 to 13.4 (p < 0.01). Competency assessments revealed that some VMWs had difficulty performing the Dengue Duo RDT, particularly in collecting the correct blood volume. This limitation may have led to false-negative dengue NS1 results. VMWs and community members were keen to broaden the skills and responsibilities of VMWs. Conclusions Deploying combo-RDTs to VMWs led to a higher utilization of their services. Difficulties performing some aspects of the Dengue Duo RDT, low positivity rates, and a lack of actionable outcomes within the existing context of VMW services suggest that alternative interventions may be better suited for VMW role expansion at this time. Overall, VMWs and community members were receptive to the expansion of the VMW role for a wider range of diseases other than malaria.
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spelling doaj-art-b59cacb8922044248f96a40e84a2d5ae2025-08-20T03:09:34ZengBMCBMC Infectious Diseases1471-23342025-05-0125111510.1186/s12879-025-11016-zOperational evaluation of the deployment of Malaria/CRP Duo and Dengue Duo rapid diagnostic tests for the management of febrile illness by village malaria workers in rural CambodiaMarc T. Visser0Dysoley Lek1Bipin Adhikari2Arjun Chandna3Moul Vanna4Sam Ol5Voeurng Bunreth6Chan Davoeung7Yok Sovann8Dafne Umans9Yoel Lubell10Rusheng Chew11Richard J. Maude12Rob W. van der Pluijm13Rupam Tripura14Naomi Waithira15Siv Sovannaroth16Michèle van Vugt17Lorenz von Seidlein18Mavuto Mukaka19Arjen Dondorp20Thomas J. Peto21James J. Callery22Centre of Tropical Medicine and Travel Medicine, Amsterdam University Medical Centres, Location AMC, University of AmsterdamNational Centre for Parasitology, Entomology and Malaria ControlCentre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of OxfordCentre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of OxfordAction for Health DevelopmentAction for Health DevelopmentBattambang Provincial Health DepartmentBattambang Provincial Health DepartmentPailin Provincial Health DepartmentCentre of Tropical Medicine and Travel Medicine, Amsterdam University Medical Centres, Location AMC, University of AmsterdamCentre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of OxfordCentre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of OxfordCentre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of OxfordCentre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of OxfordCentre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of OxfordCentre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of OxfordNational Centre for Parasitology, Entomology and Malaria ControlCentre of Tropical Medicine and Travel Medicine, Amsterdam University Medical Centres, Location AMC, University of AmsterdamCentre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of OxfordCentre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of OxfordCentre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of OxfordCentre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of OxfordCentre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of OxfordAbstract Introduction The decline in malaria cases in Cambodia has led to a relative increase in non-malarial febrile illness. In rural Cambodia, village malaria workers (VMWs) provide early diagnosis and treatment for malaria, but their role and relevance are diminishing as malaria cases decline. Expanding VMW roles would ensure continued utilisation of their services until malaria elimination is achieved and strengthen community health services. Methods A mixed methods operational research study was implemented to evaluate the use of two combination-RDTs (combo-RDTs) as an expansion of the VMW role, enabling VMWs in Cambodia to test for diseases other than malaria for the first time. VMWs in 78 villages in Battambang and Pailin Provinces were trained and provided with either a Malaria/CRP Duo or Dengue Duo RDT to assess febrile patients. Data were collected on VMW consultations, and combo-RDT usage and results. Focus group discussions (FGDs) and competency assessments of combo-RDT usage were conducted with VMWs. The main objectives were to determine whether VMWs could perform these combo-RDTs correctly and follow management algorithms, and whether deployment had an impact on VMW consultation rates. Perspectives concerning role expansion and the feasibility of conducting additional tests were also explored. Results Between June 2022, and May 2023, a total of 2,425 febrile patients were assessed with either a Malaria/CRP Duo or Dengue Duo RDT. Active dengue infection (NS1- and/or IgM-positive) was identified in 1.2% (11/915) of patients. Positive CRP results (> 20 mg/L) were found for 3.2% (48/1,510) of patients. Following deployment, there was an immediate mean increase of 4.4 VMW consultations per month, from 9.0 to 13.4 (p < 0.01). Competency assessments revealed that some VMWs had difficulty performing the Dengue Duo RDT, particularly in collecting the correct blood volume. This limitation may have led to false-negative dengue NS1 results. VMWs and community members were keen to broaden the skills and responsibilities of VMWs. Conclusions Deploying combo-RDTs to VMWs led to a higher utilization of their services. Difficulties performing some aspects of the Dengue Duo RDT, low positivity rates, and a lack of actionable outcomes within the existing context of VMW services suggest that alternative interventions may be better suited for VMW role expansion at this time. Overall, VMWs and community members were receptive to the expansion of the VMW role for a wider range of diseases other than malaria.https://doi.org/10.1186/s12879-025-11016-zVillage malaria workerCommunity health workerRDTMalariaDengueCRP
spellingShingle Marc T. Visser
Dysoley Lek
Bipin Adhikari
Arjun Chandna
Moul Vanna
Sam Ol
Voeurng Bunreth
Chan Davoeung
Yok Sovann
Dafne Umans
Yoel Lubell
Rusheng Chew
Richard J. Maude
Rob W. van der Pluijm
Rupam Tripura
Naomi Waithira
Siv Sovannaroth
Michèle van Vugt
Lorenz von Seidlein
Mavuto Mukaka
Arjen Dondorp
Thomas J. Peto
James J. Callery
Operational evaluation of the deployment of Malaria/CRP Duo and Dengue Duo rapid diagnostic tests for the management of febrile illness by village malaria workers in rural Cambodia
BMC Infectious Diseases
Village malaria worker
Community health worker
RDT
Malaria
Dengue
CRP
title Operational evaluation of the deployment of Malaria/CRP Duo and Dengue Duo rapid diagnostic tests for the management of febrile illness by village malaria workers in rural Cambodia
title_full Operational evaluation of the deployment of Malaria/CRP Duo and Dengue Duo rapid diagnostic tests for the management of febrile illness by village malaria workers in rural Cambodia
title_fullStr Operational evaluation of the deployment of Malaria/CRP Duo and Dengue Duo rapid diagnostic tests for the management of febrile illness by village malaria workers in rural Cambodia
title_full_unstemmed Operational evaluation of the deployment of Malaria/CRP Duo and Dengue Duo rapid diagnostic tests for the management of febrile illness by village malaria workers in rural Cambodia
title_short Operational evaluation of the deployment of Malaria/CRP Duo and Dengue Duo rapid diagnostic tests for the management of febrile illness by village malaria workers in rural Cambodia
title_sort operational evaluation of the deployment of malaria crp duo and dengue duo rapid diagnostic tests for the management of febrile illness by village malaria workers in rural cambodia
topic Village malaria worker
Community health worker
RDT
Malaria
Dengue
CRP
url https://doi.org/10.1186/s12879-025-11016-z
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