Thiacloprid exposure disrupts the gut-liver axis and induces liver dysfunction in the Reeves' turtles (Mauremys reevesii)
As one of the neonicotinoid insecticides, thiacloprid (THI) is extensively used in agriculture and frequently detected in various aquatic environments, posing a potential threat to aquatic organisms. However, the effects of THI exposure on aquatic turtles remain unknown. In this study, we focused on...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325000466 |
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| author | Shuqin Lin Yunjuan Xiao Siyu Li Liyan Tang Haitao Shi Meiling Hong Li Ding |
| author_facet | Shuqin Lin Yunjuan Xiao Siyu Li Liyan Tang Haitao Shi Meiling Hong Li Ding |
| author_sort | Shuqin Lin |
| collection | DOAJ |
| description | As one of the neonicotinoid insecticides, thiacloprid (THI) is extensively used in agriculture and frequently detected in various aquatic environments, posing a potential threat to aquatic organisms. However, the effects of THI exposure on aquatic turtles remain unknown. In this study, we focused on investigating whether THI has a toxic effect on the gut-liver axis in aquatic turtles. The Reeves' turtles (Mauremys reevesii) were exposed to 0.0178 μM, 6 μM, and 60 μM THI for 5 consecutive weeks. The results revealed that THI altered the composition of intestinal flora, with a decrease in the relative abundance of Romboutsia, and an increase in Clostridium_sensu_stricto_1, Cetobacterium, Enterococcus. This disruption of the intestinal barrier led to an increase in lipopolysaccharide (LPS), THI, and other harmful substances entering the liver. Metabolomic and transcriptomic analyses indicated that metabolic dysregulation and differences in gene expression were concentrated in amino acid metabolism and lipid metabolism, ultimately resulting in severe liver damage and steatosis. Furthermore, elevated levels of liver function indicators, including aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total bile acid (TBA), and triglyceride (TG), were positively correlated with increased THI concentrations. Our findings demonstrate that THI impairs the intestinal barrier and causes liver dysfunction and damage in turtles, providing new insights into evaluating the toxic effects of thiacloprid on aquatic organisms. |
| format | Article |
| id | doaj-art-b59ae9fdfa6647149040cb50b5825734 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-b59ae9fdfa6647149040cb50b58257342025-01-23T05:26:11ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01289117710Thiacloprid exposure disrupts the gut-liver axis and induces liver dysfunction in the Reeves' turtles (Mauremys reevesii)Shuqin Lin0Yunjuan Xiao1Siyu Li2Liyan Tang3Haitao Shi4Meiling Hong5Li Ding6Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou, ChinaMinistry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou, ChinaMinistry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou, ChinaMinistry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou, ChinaMinistry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou, ChinaCorresponding authors.; Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou, ChinaCorresponding authors.; Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou, ChinaAs one of the neonicotinoid insecticides, thiacloprid (THI) is extensively used in agriculture and frequently detected in various aquatic environments, posing a potential threat to aquatic organisms. However, the effects of THI exposure on aquatic turtles remain unknown. In this study, we focused on investigating whether THI has a toxic effect on the gut-liver axis in aquatic turtles. The Reeves' turtles (Mauremys reevesii) were exposed to 0.0178 μM, 6 μM, and 60 μM THI for 5 consecutive weeks. The results revealed that THI altered the composition of intestinal flora, with a decrease in the relative abundance of Romboutsia, and an increase in Clostridium_sensu_stricto_1, Cetobacterium, Enterococcus. This disruption of the intestinal barrier led to an increase in lipopolysaccharide (LPS), THI, and other harmful substances entering the liver. Metabolomic and transcriptomic analyses indicated that metabolic dysregulation and differences in gene expression were concentrated in amino acid metabolism and lipid metabolism, ultimately resulting in severe liver damage and steatosis. Furthermore, elevated levels of liver function indicators, including aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total bile acid (TBA), and triglyceride (TG), were positively correlated with increased THI concentrations. Our findings demonstrate that THI impairs the intestinal barrier and causes liver dysfunction and damage in turtles, providing new insights into evaluating the toxic effects of thiacloprid on aquatic organisms.http://www.sciencedirect.com/science/article/pii/S0147651325000466Neonicotinoid insecticidesTurtlesGut microorganismsHepatotoxicityGut-liver axis |
| spellingShingle | Shuqin Lin Yunjuan Xiao Siyu Li Liyan Tang Haitao Shi Meiling Hong Li Ding Thiacloprid exposure disrupts the gut-liver axis and induces liver dysfunction in the Reeves' turtles (Mauremys reevesii) Ecotoxicology and Environmental Safety Neonicotinoid insecticides Turtles Gut microorganisms Hepatotoxicity Gut-liver axis |
| title | Thiacloprid exposure disrupts the gut-liver axis and induces liver dysfunction in the Reeves' turtles (Mauremys reevesii) |
| title_full | Thiacloprid exposure disrupts the gut-liver axis and induces liver dysfunction in the Reeves' turtles (Mauremys reevesii) |
| title_fullStr | Thiacloprid exposure disrupts the gut-liver axis and induces liver dysfunction in the Reeves' turtles (Mauremys reevesii) |
| title_full_unstemmed | Thiacloprid exposure disrupts the gut-liver axis and induces liver dysfunction in the Reeves' turtles (Mauremys reevesii) |
| title_short | Thiacloprid exposure disrupts the gut-liver axis and induces liver dysfunction in the Reeves' turtles (Mauremys reevesii) |
| title_sort | thiacloprid exposure disrupts the gut liver axis and induces liver dysfunction in the reeves turtles mauremys reevesii |
| topic | Neonicotinoid insecticides Turtles Gut microorganisms Hepatotoxicity Gut-liver axis |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325000466 |
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