MePHD1.2 affects the synthesis of cyanogenic glycosides by regulating transcription of MeCYP79D2 in cassava
The high content of cyanogenic glycosides (CG) in cassava tubers affects food safety. CG are involved in the plant growth and development and protect cassava leaves from herbivorous predators. However, the regulatory mechanism of CG biosynthesis remains poorly understood. Here, yeast one-hybrid assa...
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KeAi Communications Co., Ltd.
2025-02-01
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| Series: | Crop Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514124002526 |
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| author | Mengtao Li Xiao Zhao Yajun Li Yuanchao Li Xiaoye Zhao Weitao Mai Luqman Khan Qibing Liang Qingchun Yin Wenquan Wang Jinping Liu Xin Chen |
| author_facet | Mengtao Li Xiao Zhao Yajun Li Yuanchao Li Xiaoye Zhao Weitao Mai Luqman Khan Qibing Liang Qingchun Yin Wenquan Wang Jinping Liu Xin Chen |
| author_sort | Mengtao Li |
| collection | DOAJ |
| description | The high content of cyanogenic glycosides (CG) in cassava tubers affects food safety. CG are involved in the plant growth and development and protect cassava leaves from herbivorous predators. However, the regulatory mechanism of CG biosynthesis remains poorly understood. Here, yeast one-hybrid assays were performed using a mixed cDNA library of cassava tubers and leaves as prey and the promoter of MeCYP79D2 as bait. MeCYP79D2, a cytochrome P450 protein, is the rate-limiting enzyme for CG synthesis in cassava. From this information, a candidate regulator of MeCYP79D2 was selected and identified as transcription factor MePHD1.2. MePHD1.2, located in the nucleus and exhibiting an inhibitory transcription activity directly bound to an AT-rich motif in the promoter of MeCYP79D2. In cassava, the transcriptional activity of MeCYP79D2 was considerably enhanced in mephd1.2 mutant lines leading to increased linamarin and lotaustralin contents. Deletion of MePHD1.2 promoted the production of CGs in cassava and decreased transcription inhibition on MeCYP79D2, exposing a novel regulatory module governing biosynthesis of CGs. |
| format | Article |
| id | doaj-art-b5968cd0719d49ffb9d2e6404517a12e |
| institution | DOAJ |
| issn | 2214-5141 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Crop Journal |
| spelling | doaj-art-b5968cd0719d49ffb9d2e6404517a12e2025-08-20T03:16:18ZengKeAi Communications Co., Ltd.Crop Journal2214-51412025-02-0113120421410.1016/j.cj.2024.12.003MePHD1.2 affects the synthesis of cyanogenic glycosides by regulating transcription of MeCYP79D2 in cassavaMengtao Li0Xiao Zhao1Yajun Li2Yuanchao Li3Xiaoye Zhao4Weitao Mai5Luqman Khan6Qibing Liang7Qingchun Yin8Wenquan Wang9Jinping Liu10Xin Chen11Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, National Key Laboratory for Tropical Crop Breeding, Sanya 572025, Hainan, China; Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, ChinaInstitute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, ChinaInstitute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, National Key Laboratory for Tropical Crop Breeding, Sanya 572025, Hainan, China; Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, China; Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya 572025, Hainan, ChinaInstitute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, China; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/College of Life Science and Technology, Guangxi University, Nanning 530004, Guangxi, ChinaHaikou Branch of Hainan Food and Drug Inspection Institute, Haikou 570311, Hainan, ChinaInstitute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, National Key Laboratory for Tropical Crop Breeding, Sanya 572025, Hainan, China; Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, ChinaInstitute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, National Key Laboratory for Tropical Crop Breeding, Sanya 572025, Hainan, ChinaHaikou Branch of Hainan Food and Drug Inspection Institute, Haikou 570311, Hainan, ChinaHainan Institute for Food Control, Key Laboratory of Tropical Fruits and Vegetables Quality and Safety for State Market Regulation, Haikou 570314, Hainan, ChinaInstitute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, National Key Laboratory for Tropical Crop Breeding, Sanya 572025, Hainan, China; Corresponding authors.Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, National Key Laboratory for Tropical Crop Breeding, Sanya 572025, Hainan, China; Corresponding authors.Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, National Key Laboratory for Tropical Crop Breeding, Sanya 572025, Hainan, China; Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, China; Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya 572025, Hainan, China; Corresponding authors.The high content of cyanogenic glycosides (CG) in cassava tubers affects food safety. CG are involved in the plant growth and development and protect cassava leaves from herbivorous predators. However, the regulatory mechanism of CG biosynthesis remains poorly understood. Here, yeast one-hybrid assays were performed using a mixed cDNA library of cassava tubers and leaves as prey and the promoter of MeCYP79D2 as bait. MeCYP79D2, a cytochrome P450 protein, is the rate-limiting enzyme for CG synthesis in cassava. From this information, a candidate regulator of MeCYP79D2 was selected and identified as transcription factor MePHD1.2. MePHD1.2, located in the nucleus and exhibiting an inhibitory transcription activity directly bound to an AT-rich motif in the promoter of MeCYP79D2. In cassava, the transcriptional activity of MeCYP79D2 was considerably enhanced in mephd1.2 mutant lines leading to increased linamarin and lotaustralin contents. Deletion of MePHD1.2 promoted the production of CGs in cassava and decreased transcription inhibition on MeCYP79D2, exposing a novel regulatory module governing biosynthesis of CGs.http://www.sciencedirect.com/science/article/pii/S2214514124002526Cyanogenic glycosidesCytochrome P450Manihot esculentaMePHD1.2 |
| spellingShingle | Mengtao Li Xiao Zhao Yajun Li Yuanchao Li Xiaoye Zhao Weitao Mai Luqman Khan Qibing Liang Qingchun Yin Wenquan Wang Jinping Liu Xin Chen MePHD1.2 affects the synthesis of cyanogenic glycosides by regulating transcription of MeCYP79D2 in cassava Crop Journal Cyanogenic glycosides Cytochrome P450 Manihot esculenta MePHD1.2 |
| title | MePHD1.2 affects the synthesis of cyanogenic glycosides by regulating transcription of MeCYP79D2 in cassava |
| title_full | MePHD1.2 affects the synthesis of cyanogenic glycosides by regulating transcription of MeCYP79D2 in cassava |
| title_fullStr | MePHD1.2 affects the synthesis of cyanogenic glycosides by regulating transcription of MeCYP79D2 in cassava |
| title_full_unstemmed | MePHD1.2 affects the synthesis of cyanogenic glycosides by regulating transcription of MeCYP79D2 in cassava |
| title_short | MePHD1.2 affects the synthesis of cyanogenic glycosides by regulating transcription of MeCYP79D2 in cassava |
| title_sort | mephd1 2 affects the synthesis of cyanogenic glycosides by regulating transcription of mecyp79d2 in cassava |
| topic | Cyanogenic glycosides Cytochrome P450 Manihot esculenta MePHD1.2 |
| url | http://www.sciencedirect.com/science/article/pii/S2214514124002526 |
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