Serum thioredoxin and lactoferrin in rheumatoid arthritis and their association with rheumatoid factor
Background: Thioredoxin (Trx) and lactoferrin (Lf) are multifunctional proteins that are part of the body's antioxidant defence and counteract oxidative tissue damage. Our study aims to investigate Trx and LF levels in the serum of rheumatoid arthritis (RA) patients and establish the associatio...
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Society of Medical Biochemists of Serbia, Belgrade
2025-01-01
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| Series: | Journal of Medical Biochemistry |
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| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2025/1452-82582502312D.pdf |
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| author | Delcheva Ginka Stefanova Katya Stankova Teodora Maneva Ana |
| author_facet | Delcheva Ginka Stefanova Katya Stankova Teodora Maneva Ana |
| author_sort | Delcheva Ginka |
| collection | DOAJ |
| description | Background: Thioredoxin (Trx) and lactoferrin (Lf) are multifunctional proteins that are part of the body's antioxidant defence and counteract oxidative tissue damage. Our study aims to investigate Trx and LF levels in the serum of rheumatoid arthritis (RA) patients and establish the association of these proteins with the rheumatoid factor (RF) and other disease markers. Methods: The study included 114 patients with RA and 42 healthy subjects. Serum concentrations of Trx 1, LF, RF, anti-cyclic citrullinated peptide (anti-CCP) antibodies, Creactive protein (CRP), and IL-6 were determined using commercially available ELISA kits. Results: Serum thioredoxin 1 levels in RA were significantly higher compared to the control group, 36.4 (29.6-40.2), ng/mL versus 19.0 (16.3-26.8), ng/mL, p<0.0001. Serum lactoferrin levels were elevated in RA compared to the control group. However, the difference was not statistically significant, 579.6 (312.8-947.5) ng/mL versus 519.0 (262.5-928.0) ng/mL. We found substantial negative Trx 1 and LF correlations with rheumatoid factor in RA (r=-0.254, p=0.05 and r=-0.238, p=0.014, respectively). The correlations of thioredoxin and lactoferrin with other disease markers, such as anti-CCP antibodies, DAS28, erythrocyte sedimentation rate (ESR), CRP, and IL6 were not statistically significant. A strong positive correlation between Trx 1 and LF was observed in the study group of RA patients (r=0.519, p<0.0001) but not in the control group. Conclusions: Thioredoxin and lactoferrin were associated with rheumatoid factor but not with anti-CCP antibodies and systemic disease activity; therefore, the two proteins may serve as new biomarkers for assessing pathological changes and monitoring disease severity and progression in RA. |
| format | Article |
| id | doaj-art-b5906ef3eae0468cbc22e8ad5cadfdad |
| institution | DOAJ |
| issn | 1452-8258 1452-8266 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Society of Medical Biochemists of Serbia, Belgrade |
| record_format | Article |
| series | Journal of Medical Biochemistry |
| spelling | doaj-art-b5906ef3eae0468cbc22e8ad5cadfdad2025-08-20T02:54:50ZengSociety of Medical Biochemists of Serbia, BelgradeJournal of Medical Biochemistry1452-82581452-82662025-01-0144231231810.5937/jomb0-447581452-82582502312DSerum thioredoxin and lactoferrin in rheumatoid arthritis and their association with rheumatoid factorDelcheva Ginka0Stefanova Katya1Stankova Teodora2Maneva Ana3Medical University of Plovdiv, Faculty of Pharmacy, Department of Medical Biochemistry, Plovdiv, BulgariaMedical University of Plovdiv, Faculty of Pharmacy, Department of Medical Biochemistry, Plovdiv, BulgariaMedical University of Plovdiv, Faculty of Pharmacy, Department of Medical Biochemistry, Plovdiv, BulgariaMedical University of Plovdiv, Faculty of Pharmacy, Department of Medical Biochemistry, Plovdiv, BulgariaBackground: Thioredoxin (Trx) and lactoferrin (Lf) are multifunctional proteins that are part of the body's antioxidant defence and counteract oxidative tissue damage. Our study aims to investigate Trx and LF levels in the serum of rheumatoid arthritis (RA) patients and establish the association of these proteins with the rheumatoid factor (RF) and other disease markers. Methods: The study included 114 patients with RA and 42 healthy subjects. Serum concentrations of Trx 1, LF, RF, anti-cyclic citrullinated peptide (anti-CCP) antibodies, Creactive protein (CRP), and IL-6 were determined using commercially available ELISA kits. Results: Serum thioredoxin 1 levels in RA were significantly higher compared to the control group, 36.4 (29.6-40.2), ng/mL versus 19.0 (16.3-26.8), ng/mL, p<0.0001. Serum lactoferrin levels were elevated in RA compared to the control group. However, the difference was not statistically significant, 579.6 (312.8-947.5) ng/mL versus 519.0 (262.5-928.0) ng/mL. We found substantial negative Trx 1 and LF correlations with rheumatoid factor in RA (r=-0.254, p=0.05 and r=-0.238, p=0.014, respectively). The correlations of thioredoxin and lactoferrin with other disease markers, such as anti-CCP antibodies, DAS28, erythrocyte sedimentation rate (ESR), CRP, and IL6 were not statistically significant. A strong positive correlation between Trx 1 and LF was observed in the study group of RA patients (r=0.519, p<0.0001) but not in the control group. Conclusions: Thioredoxin and lactoferrin were associated with rheumatoid factor but not with anti-CCP antibodies and systemic disease activity; therefore, the two proteins may serve as new biomarkers for assessing pathological changes and monitoring disease severity and progression in RA.https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2025/1452-82582502312D.pdflactoferrinoxidative stressrheumatoid arthritisrheumatoid factorthioredoxin 1 |
| spellingShingle | Delcheva Ginka Stefanova Katya Stankova Teodora Maneva Ana Serum thioredoxin and lactoferrin in rheumatoid arthritis and their association with rheumatoid factor Journal of Medical Biochemistry lactoferrin oxidative stress rheumatoid arthritis rheumatoid factor thioredoxin 1 |
| title | Serum thioredoxin and lactoferrin in rheumatoid arthritis and their association with rheumatoid factor |
| title_full | Serum thioredoxin and lactoferrin in rheumatoid arthritis and their association with rheumatoid factor |
| title_fullStr | Serum thioredoxin and lactoferrin in rheumatoid arthritis and their association with rheumatoid factor |
| title_full_unstemmed | Serum thioredoxin and lactoferrin in rheumatoid arthritis and their association with rheumatoid factor |
| title_short | Serum thioredoxin and lactoferrin in rheumatoid arthritis and their association with rheumatoid factor |
| title_sort | serum thioredoxin and lactoferrin in rheumatoid arthritis and their association with rheumatoid factor |
| topic | lactoferrin oxidative stress rheumatoid arthritis rheumatoid factor thioredoxin 1 |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2025/1452-82582502312D.pdf |
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