Functional Characteristics of Multipotent Mesenchymal Stromal Cells from Pituitary Adenomas
Pituitary adenomas are one of the most common endocrine and intracranial neoplasms. Although they are theoretically monoclonal in origin, several studies have shown that they contain different multipotent cell types that are thought to play an important role in tumor initiation, maintenance, and rec...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/7103720 |
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| author | Kaspars Megnis Ilona Mandrika Ramona Petrovska Janis Stukens Vita Rovite Inga Balcere Laima Sabine Jansone Raitis Peculis Valdis Pirags Janis Klovins |
| author_facet | Kaspars Megnis Ilona Mandrika Ramona Petrovska Janis Stukens Vita Rovite Inga Balcere Laima Sabine Jansone Raitis Peculis Valdis Pirags Janis Klovins |
| author_sort | Kaspars Megnis |
| collection | DOAJ |
| description | Pituitary adenomas are one of the most common endocrine and intracranial neoplasms. Although they are theoretically monoclonal in origin, several studies have shown that they contain different multipotent cell types that are thought to play an important role in tumor initiation, maintenance, and recurrence after therapy. In the present study, we isolated and characterized cell populations from seven pituitary somatotroph, nonhormonal, and lactotroph adenomas. The obtained cells showed characteristics of multipotent mesenchymal stromal cells as observed by cell morphology, cell surface marker CD90, CD105, CD44, and vimentin expression, as well as differentiation to osteogenic and adipogenic lineages. They are capable of growth and passaging under standard laboratory cell culture conditions and do not manifest any hormonal cell characteristics. Multipotent mesenchymal stromal cells are present in pituitary adenomas regardless of their clinical manifestation and show no considerable expression of somatostatin 1–5 and dopamine 2 receptors. Most likely obtained cells are a part of tissue-supportive cells in pituitary adenoma microenvironment. |
| format | Article |
| id | doaj-art-b5885d393dc64ce0ba5f10e38e77773c |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-b5885d393dc64ce0ba5f10e38e77773c2025-08-20T02:23:24ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/71037207103720Functional Characteristics of Multipotent Mesenchymal Stromal Cells from Pituitary AdenomasKaspars Megnis0Ilona Mandrika1Ramona Petrovska2Janis Stukens3Vita Rovite4Inga Balcere5Laima Sabine Jansone6Raitis Peculis7Valdis Pirags8Janis Klovins9Latvian Biomedical Research and Study Centre, Ratsupites Street 1 k-1, Riga LV-1067, LatviaLatvian Biomedical Research and Study Centre, Ratsupites Street 1 k-1, Riga LV-1067, LatviaLatvian Biomedical Research and Study Centre, Ratsupites Street 1 k-1, Riga LV-1067, LatviaClinic of Neurosurgery, Pauls Stradins Clinical University Hospital, 13 Pilsonu Street, Riga LV-1002, LatviaLatvian Biomedical Research and Study Centre, Ratsupites Street 1 k-1, Riga LV-1067, LatviaCentre of Endocrinology, Pauls Stradins Clinical University Hospital, 13 Pilsonu Street, Riga LV-1002, LatviaLatvian Biomedical Research and Study Centre, Ratsupites Street 1 k-1, Riga LV-1067, LatviaLatvian Biomedical Research and Study Centre, Ratsupites Street 1 k-1, Riga LV-1067, LatviaLatvian Biomedical Research and Study Centre, Ratsupites Street 1 k-1, Riga LV-1067, LatviaLatvian Biomedical Research and Study Centre, Ratsupites Street 1 k-1, Riga LV-1067, LatviaPituitary adenomas are one of the most common endocrine and intracranial neoplasms. Although they are theoretically monoclonal in origin, several studies have shown that they contain different multipotent cell types that are thought to play an important role in tumor initiation, maintenance, and recurrence after therapy. In the present study, we isolated and characterized cell populations from seven pituitary somatotroph, nonhormonal, and lactotroph adenomas. The obtained cells showed characteristics of multipotent mesenchymal stromal cells as observed by cell morphology, cell surface marker CD90, CD105, CD44, and vimentin expression, as well as differentiation to osteogenic and adipogenic lineages. They are capable of growth and passaging under standard laboratory cell culture conditions and do not manifest any hormonal cell characteristics. Multipotent mesenchymal stromal cells are present in pituitary adenomas regardless of their clinical manifestation and show no considerable expression of somatostatin 1–5 and dopamine 2 receptors. Most likely obtained cells are a part of tissue-supportive cells in pituitary adenoma microenvironment.http://dx.doi.org/10.1155/2016/7103720 |
| spellingShingle | Kaspars Megnis Ilona Mandrika Ramona Petrovska Janis Stukens Vita Rovite Inga Balcere Laima Sabine Jansone Raitis Peculis Valdis Pirags Janis Klovins Functional Characteristics of Multipotent Mesenchymal Stromal Cells from Pituitary Adenomas Stem Cells International |
| title | Functional Characteristics of Multipotent Mesenchymal Stromal Cells from Pituitary Adenomas |
| title_full | Functional Characteristics of Multipotent Mesenchymal Stromal Cells from Pituitary Adenomas |
| title_fullStr | Functional Characteristics of Multipotent Mesenchymal Stromal Cells from Pituitary Adenomas |
| title_full_unstemmed | Functional Characteristics of Multipotent Mesenchymal Stromal Cells from Pituitary Adenomas |
| title_short | Functional Characteristics of Multipotent Mesenchymal Stromal Cells from Pituitary Adenomas |
| title_sort | functional characteristics of multipotent mesenchymal stromal cells from pituitary adenomas |
| url | http://dx.doi.org/10.1155/2016/7103720 |
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