MICROSATELLITE GENOTYPING OF DON LOCAL GRAPE (VITIS VINIFERA L.) VARIETIES
Local traditional varieties constitute an important portion of grape genetic resources. DNA profiles for microsatellite loci VVMD5, VVMD7, VVMD27, VVS2, VrZAG62, and VrZAG79 were obtained for 11 local grape cultivars grown in the Don region. These SSR-markers are recommended as basic for Vitis vinif...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2015-01-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/278 |
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| author | E. T. Ilnitskaya S. V. Tokmakov I. I. Suprun |
| author_facet | E. T. Ilnitskaya S. V. Tokmakov I. I. Suprun |
| author_sort | E. T. Ilnitskaya |
| collection | DOAJ |
| description | Local traditional varieties constitute an important portion of grape genetic resources. DNA profiles for microsatellite loci VVMD5, VVMD7, VVMD27, VVS2, VrZAG62, and VrZAG79 were obtained for 11 local grape cultivars grown in the Don region. These SSR-markers are recommended as basic for Vitis vinifera genotyping. Chardonnay and Cabernet Sauvignon were used as reference cultivars. The markers were combined in multiple sets for simultaneous evaluation of two loci. The total number of different alleles per locus varied from 5 (VVS2, VMD7) to 11 (VVMD5) within the Don cultivars analyzed. The cultivars possessed different sets of allele combinations for loci under study. The genotyping results were subject to cluster analysis. Sibir’kovyi and Pukhlyakovskiy belyi showed the greatest proximity to each other. Molecular studies of local southern Russia grape cultivars will be continued. |
| format | Article |
| id | doaj-art-b58324aac6dc45498f9c6e94432ea0cc |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-b58324aac6dc45498f9c6e94432ea0cc2025-02-01T09:58:01ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592015-01-01183523529262MICROSATELLITE GENOTYPING OF DON LOCAL GRAPE (VITIS VINIFERA L.) VARIETIESE. T. Ilnitskaya0S. V. Tokmakov1I. I. Suprun2North Caucasian Zonal Research Institute for Horticulture and Viticulture, Russian Academy of Agricultural Sciences, Krasnodar, RussiaNorth Caucasian Zonal Research Institute for Horticulture and Viticulture, Russian Academy of Agricultural Sciences, Krasnodar, RussiaNorth Caucasian Zonal Research Institute for Horticulture and Viticulture, Russian Academy of Agricultural Sciences, Krasnodar, RussiaLocal traditional varieties constitute an important portion of grape genetic resources. DNA profiles for microsatellite loci VVMD5, VVMD7, VVMD27, VVS2, VrZAG62, and VrZAG79 were obtained for 11 local grape cultivars grown in the Don region. These SSR-markers are recommended as basic for Vitis vinifera genotyping. Chardonnay and Cabernet Sauvignon were used as reference cultivars. The markers were combined in multiple sets for simultaneous evaluation of two loci. The total number of different alleles per locus varied from 5 (VVS2, VMD7) to 11 (VVMD5) within the Don cultivars analyzed. The cultivars possessed different sets of allele combinations for loci under study. The genotyping results were subject to cluster analysis. Sibir’kovyi and Pukhlyakovskiy belyi showed the greatest proximity to each other. Molecular studies of local southern Russia grape cultivars will be continued.https://vavilov.elpub.ru/jour/article/view/278vitis viniferalocal grape cultivarsssr markersgenotyping |
| spellingShingle | E. T. Ilnitskaya S. V. Tokmakov I. I. Suprun MICROSATELLITE GENOTYPING OF DON LOCAL GRAPE (VITIS VINIFERA L.) VARIETIES Вавиловский журнал генетики и селекции vitis vinifera local grape cultivars ssr markers genotyping |
| title | MICROSATELLITE GENOTYPING OF DON LOCAL GRAPE (VITIS VINIFERA L.) VARIETIES |
| title_full | MICROSATELLITE GENOTYPING OF DON LOCAL GRAPE (VITIS VINIFERA L.) VARIETIES |
| title_fullStr | MICROSATELLITE GENOTYPING OF DON LOCAL GRAPE (VITIS VINIFERA L.) VARIETIES |
| title_full_unstemmed | MICROSATELLITE GENOTYPING OF DON LOCAL GRAPE (VITIS VINIFERA L.) VARIETIES |
| title_short | MICROSATELLITE GENOTYPING OF DON LOCAL GRAPE (VITIS VINIFERA L.) VARIETIES |
| title_sort | microsatellite genotyping of don local grape vitis vinifera l varieties |
| topic | vitis vinifera local grape cultivars ssr markers genotyping |
| url | https://vavilov.elpub.ru/jour/article/view/278 |
| work_keys_str_mv | AT etilnitskaya microsatellitegenotypingofdonlocalgrapevitisviniferalvarieties AT svtokmakov microsatellitegenotypingofdonlocalgrapevitisviniferalvarieties AT iisuprun microsatellitegenotypingofdonlocalgrapevitisviniferalvarieties |