Melatonin improves the in vitro growth of bovine oocytes collected from early antral follicles by maintaining oocyte‐cumulus cell communication
Abstract Purpose In vitro, oocyte development is susceptible to oxidative stress, which leads to endoplasmic reticulum (ER) stress. This study investigated whether the antioxidant melatonin attenuates ER stress and maintains oocyte‐cumulus cell communication during the in vitro growth (IVG) of bovin...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Reproductive Medicine and Biology |
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| Online Access: | https://doi.org/10.1002/rmb2.12629 |
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| author | Md Nuronnabi Islam Fumio Ebara Toshihiro Konno Hideki Tatemoto Ken‐ichi Yamanaka |
| author_facet | Md Nuronnabi Islam Fumio Ebara Toshihiro Konno Hideki Tatemoto Ken‐ichi Yamanaka |
| author_sort | Md Nuronnabi Islam |
| collection | DOAJ |
| description | Abstract Purpose In vitro, oocyte development is susceptible to oxidative stress, which leads to endoplasmic reticulum (ER) stress. This study investigated whether the antioxidant melatonin attenuates ER stress and maintains oocyte‐cumulus cell communication during the in vitro growth (IVG) of bovine oocytes. Methods Oocyte‐granulosa cell complexes (OGCs) were harvested from slaughterhouse‐derived ovaries and grown in vitro for 5 d at 38.5°C in 5% CO2 humidified air. Melatonin (10−7, 10−9, or 10−11 M) was added to the culture medium. Results Oocyte diameter increased on day 5 from its initial value in all groups. The antrum formation rate was significantly higher in the 10−9 M melatonin‐treated group than in the control. The melatonin‐treated group showed reduced oxidative stress and increased gap junction communication compared with the control. ER stress‐related genes in OGCs were significantly downregulated in the 10−9 M melatonin‐treated group compared with those in the control. No significant changes were found in subsequent maturation among groups; however, 10−9 M melatonin treatment during IVG and IVM increased the maturation rate compared with that in the control. Conclusions Melatonin reduces oxidative stress, which attenuates ER stress in OGCs during IVG of bovine oocytes and may improve IVG efficiency in assisted reproductive technology. |
| format | Article |
| id | doaj-art-b557d62b9d0742dda4d1eef5e4e5db1a |
| institution | Kabale University |
| issn | 1445-5781 1447-0578 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
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| series | Reproductive Medicine and Biology |
| spelling | doaj-art-b557d62b9d0742dda4d1eef5e4e5db1a2025-08-20T06:16:47ZengWileyReproductive Medicine and Biology1445-57811447-05782025-01-01241n/an/a10.1002/rmb2.12629Melatonin improves the in vitro growth of bovine oocytes collected from early antral follicles by maintaining oocyte‐cumulus cell communicationMd Nuronnabi Islam0Fumio Ebara1Toshihiro Konno2Hideki Tatemoto3Ken‐ichi Yamanaka4Faculty of Agriculture Saga University Saga JapanFaculty of Agriculture Saga University Saga JapanThe United Graduate School of Agricultural Sciences Kagoshima University Kagoshima JapanThe United Graduate School of Agricultural Sciences Kagoshima University Kagoshima JapanFaculty of Agriculture Saga University Saga JapanAbstract Purpose In vitro, oocyte development is susceptible to oxidative stress, which leads to endoplasmic reticulum (ER) stress. This study investigated whether the antioxidant melatonin attenuates ER stress and maintains oocyte‐cumulus cell communication during the in vitro growth (IVG) of bovine oocytes. Methods Oocyte‐granulosa cell complexes (OGCs) were harvested from slaughterhouse‐derived ovaries and grown in vitro for 5 d at 38.5°C in 5% CO2 humidified air. Melatonin (10−7, 10−9, or 10−11 M) was added to the culture medium. Results Oocyte diameter increased on day 5 from its initial value in all groups. The antrum formation rate was significantly higher in the 10−9 M melatonin‐treated group than in the control. The melatonin‐treated group showed reduced oxidative stress and increased gap junction communication compared with the control. ER stress‐related genes in OGCs were significantly downregulated in the 10−9 M melatonin‐treated group compared with those in the control. No significant changes were found in subsequent maturation among groups; however, 10−9 M melatonin treatment during IVG and IVM increased the maturation rate compared with that in the control. Conclusions Melatonin reduces oxidative stress, which attenuates ER stress in OGCs during IVG of bovine oocytes and may improve IVG efficiency in assisted reproductive technology.https://doi.org/10.1002/rmb2.12629endoplasmic reticulum stressgap junctionin vitro growthmelatoninoxidative stress |
| spellingShingle | Md Nuronnabi Islam Fumio Ebara Toshihiro Konno Hideki Tatemoto Ken‐ichi Yamanaka Melatonin improves the in vitro growth of bovine oocytes collected from early antral follicles by maintaining oocyte‐cumulus cell communication Reproductive Medicine and Biology endoplasmic reticulum stress gap junction in vitro growth melatonin oxidative stress |
| title | Melatonin improves the in vitro growth of bovine oocytes collected from early antral follicles by maintaining oocyte‐cumulus cell communication |
| title_full | Melatonin improves the in vitro growth of bovine oocytes collected from early antral follicles by maintaining oocyte‐cumulus cell communication |
| title_fullStr | Melatonin improves the in vitro growth of bovine oocytes collected from early antral follicles by maintaining oocyte‐cumulus cell communication |
| title_full_unstemmed | Melatonin improves the in vitro growth of bovine oocytes collected from early antral follicles by maintaining oocyte‐cumulus cell communication |
| title_short | Melatonin improves the in vitro growth of bovine oocytes collected from early antral follicles by maintaining oocyte‐cumulus cell communication |
| title_sort | melatonin improves the in vitro growth of bovine oocytes collected from early antral follicles by maintaining oocyte cumulus cell communication |
| topic | endoplasmic reticulum stress gap junction in vitro growth melatonin oxidative stress |
| url | https://doi.org/10.1002/rmb2.12629 |
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