Analysis of the correlation between the distribution of microorganisms carried by Coix seed and fungal toxins, and the biological control of aflatoxin

Coix seed is a valuable crop with both medicinal and edible uses, significantly impacting economic and medical sectors. However, its contamination with fungi and mycotoxins poses serious health risks when used medicinally. Thus, identifying fungi in Coix seed and controlling fungal toxins is crucial...

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Main Authors: Lingxia Peng, Jiahao Fang, Lijun Yang, Gurusamy Abirami, Feng Yin, Lietao Cai, Chaojun Zhang, Yuyang Zhao, Yan Jin, Shuifeng Zhang, Huadong Sheng, Guohong Zeng, Haimin Chen, Zongsuo Liang, Xiaodan Zhang
Format: Article
Language:English
Published: Elsevier 2025-01-01
Series:Current Research in Food Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S2665927125000711
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author Lingxia Peng
Jiahao Fang
Lijun Yang
Gurusamy Abirami
Feng Yin
Lietao Cai
Chaojun Zhang
Yuyang Zhao
Yan Jin
Shuifeng Zhang
Huadong Sheng
Guohong Zeng
Haimin Chen
Zongsuo Liang
Xiaodan Zhang
author_facet Lingxia Peng
Jiahao Fang
Lijun Yang
Gurusamy Abirami
Feng Yin
Lietao Cai
Chaojun Zhang
Yuyang Zhao
Yan Jin
Shuifeng Zhang
Huadong Sheng
Guohong Zeng
Haimin Chen
Zongsuo Liang
Xiaodan Zhang
author_sort Lingxia Peng
collection DOAJ
description Coix seed is a valuable crop with both medicinal and edible uses, significantly impacting economic and medical sectors. However, its contamination with fungi and mycotoxins poses serious health risks when used medicinally. Thus, identifying fungi in Coix seed and controlling fungal toxins is crucial. This study employs high-throughput sequencing to detect fungi in Coix seed samples and analyze their correlation with mycotoxin content. Our findings showed no fungal infection from seed filling to maturity, but a diverse fungal community was present in mature seeds, the relative abundance of the dominant fungal phyla varied across different regions. In GZQL, YNFYA, and GD, the Ascomycota phylum dominated, and in LN and GXBS, Basidiomycota was the predominant phylum. These genera showed significant correlations with aflatoxins, zearalenone, and deoxynivalenol, indicating their potential as mycotoxin producers. Analysis revealed that Bacillus had a negative correlation with all detected mycotoxins. Antagonistic experiments demonstrated that Bacillus strains effectively inhibited aflatoxin production by Aspergillus flavus. This study provides essential data for preventing fungal growth and mycotoxin accumulation in Coix seed, ensuring its safe use in medicine and as food. These findings provide essential data for developing targeted strategies to prevent fungal growth and mycotoxin accumulation in Coix seed, ensuring its safe use in both medicinal and dietary applications. By enhancing our understanding of the microbial dynamics in Coix seed, we can improve food safety and public health outcomes, reinforcing the importance of continued research in this field.
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spelling doaj-art-b552b160db474ff38c8c0b8cd4f23f402025-08-20T02:39:38ZengElsevierCurrent Research in Food Science2665-92712025-01-011010104010.1016/j.crfs.2025.101040Analysis of the correlation between the distribution of microorganisms carried by Coix seed and fungal toxins, and the biological control of aflatoxinLingxia Peng0Jiahao Fang1Lijun Yang2Gurusamy Abirami3Feng Yin4Lietao Cai5Chaojun Zhang6Yuyang Zhao7Yan Jin8Shuifeng Zhang9Huadong Sheng10Guohong Zeng11Haimin Chen12Zongsuo Liang13Xiaodan Zhang14College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, ChinaCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, ChinaCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, ChinaCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, ChinaCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, ChinaR&D Center of Kanglaite, Hangzhou, 310018, ChinaR&D Center of Kanglaite, Hangzhou, 310018, ChinaState Key Lab Breeding Base Dao-Di Herbs, National Resource Center Chinese Materia Medica, Beijing, China Academy of Chinese Medical Sciences, Beijing, 100700, ChinaState Key Lab Breeding Base Dao-Di Herbs, National Resource Center Chinese Materia Medica, Beijing, China Academy of Chinese Medical Sciences, Beijing, 100700, ChinaZhejiang FangYuan Testing Group Co. LTD, Hangzhou, 310018, ChinaZhejiang FangYuan Testing Group Co. LTD, Hangzhou, 310018, ChinaCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, ChinaCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, ChinaCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, ChinaCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, China; Corresponding author.Coix seed is a valuable crop with both medicinal and edible uses, significantly impacting economic and medical sectors. However, its contamination with fungi and mycotoxins poses serious health risks when used medicinally. Thus, identifying fungi in Coix seed and controlling fungal toxins is crucial. This study employs high-throughput sequencing to detect fungi in Coix seed samples and analyze their correlation with mycotoxin content. Our findings showed no fungal infection from seed filling to maturity, but a diverse fungal community was present in mature seeds, the relative abundance of the dominant fungal phyla varied across different regions. In GZQL, YNFYA, and GD, the Ascomycota phylum dominated, and in LN and GXBS, Basidiomycota was the predominant phylum. These genera showed significant correlations with aflatoxins, zearalenone, and deoxynivalenol, indicating their potential as mycotoxin producers. Analysis revealed that Bacillus had a negative correlation with all detected mycotoxins. Antagonistic experiments demonstrated that Bacillus strains effectively inhibited aflatoxin production by Aspergillus flavus. This study provides essential data for preventing fungal growth and mycotoxin accumulation in Coix seed, ensuring its safe use in medicine and as food. These findings provide essential data for developing targeted strategies to prevent fungal growth and mycotoxin accumulation in Coix seed, ensuring its safe use in both medicinal and dietary applications. By enhancing our understanding of the microbial dynamics in Coix seed, we can improve food safety and public health outcomes, reinforcing the importance of continued research in this field.http://www.sciencedirect.com/science/article/pii/S2665927125000711Coix seedFungiMycotoxinsAssociation analysisCoix lacryma-Jobi L
spellingShingle Lingxia Peng
Jiahao Fang
Lijun Yang
Gurusamy Abirami
Feng Yin
Lietao Cai
Chaojun Zhang
Yuyang Zhao
Yan Jin
Shuifeng Zhang
Huadong Sheng
Guohong Zeng
Haimin Chen
Zongsuo Liang
Xiaodan Zhang
Analysis of the correlation between the distribution of microorganisms carried by Coix seed and fungal toxins, and the biological control of aflatoxin
Current Research in Food Science
Coix seed
Fungi
Mycotoxins
Association analysis
Coix lacryma-Jobi L
title Analysis of the correlation between the distribution of microorganisms carried by Coix seed and fungal toxins, and the biological control of aflatoxin
title_full Analysis of the correlation between the distribution of microorganisms carried by Coix seed and fungal toxins, and the biological control of aflatoxin
title_fullStr Analysis of the correlation between the distribution of microorganisms carried by Coix seed and fungal toxins, and the biological control of aflatoxin
title_full_unstemmed Analysis of the correlation between the distribution of microorganisms carried by Coix seed and fungal toxins, and the biological control of aflatoxin
title_short Analysis of the correlation between the distribution of microorganisms carried by Coix seed and fungal toxins, and the biological control of aflatoxin
title_sort analysis of the correlation between the distribution of microorganisms carried by coix seed and fungal toxins and the biological control of aflatoxin
topic Coix seed
Fungi
Mycotoxins
Association analysis
Coix lacryma-Jobi L
url http://www.sciencedirect.com/science/article/pii/S2665927125000711
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