Integrated Transcriptome and Targeted Metabolome for Resolving Flavonoid Biosynthesis in Figs (<i>Ficus carica Linn</i>.)
Figs are an edible and medicinal plant rich in polyphenols and flavonoids with unique pharmacological effects. However, the mechanism of flavonoid synthesis in figs is not clear. In this study, fig fruits of six varieties were collected for RNA sequencing and UPLC-MS data collection. The results sho...
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2025-02-01
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| author | Junting Sun Hadir Yishake Ming Wang Hao Zhang Jie Yan |
| author_facet | Junting Sun Hadir Yishake Ming Wang Hao Zhang Jie Yan |
| author_sort | Junting Sun |
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| description | Figs are an edible and medicinal plant rich in polyphenols and flavonoids with unique pharmacological effects. However, the mechanism of flavonoid synthesis in figs is not clear. In this study, fig fruits of six varieties were collected for RNA sequencing and UPLC-MS data collection. The results showed that a total of 39 differential metabolites were identified by targeted metabolomics, and their contents were determined by UPLC-MS. The clustered heat map analysis showed that most of the differential metabolites were highly accumulated in BRD and FY. A total of 62 flavonoid biosynthesis pathway genes were identified by transcriptome analysis, and <i>FcCHS</i>, <i>FcCHI</i>, <i>FcFLS</i>, <i>FcCYP</i>, and <i>FcDFR</i> were the key genes identified for the accumulation of flavonoids and flavonols in the dark-colored varieties. In addition, a total of 1671 transcription factor genes, mainly MYBs, bHLHs, and AP2/ERFs, were identified. This study will enrich the transcriptomic data of figs and provide some help in resolving the synthesis mechanism of fig flavonoids. |
| format | Article |
| id | doaj-art-b54f528c9cac4ab0b50e08bd294e7ca1 |
| institution | DOAJ |
| issn | 2079-7737 |
| language | English |
| publishDate | 2025-02-01 |
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| spelling | doaj-art-b54f528c9cac4ab0b50e08bd294e7ca12025-08-20T03:12:01ZengMDPI AGBiology2079-77372025-02-0114218410.3390/biology14020184Integrated Transcriptome and Targeted Metabolome for Resolving Flavonoid Biosynthesis in Figs (<i>Ficus carica Linn</i>.)Junting Sun0Hadir Yishake1Ming Wang2Hao Zhang3Jie Yan4College of Life Sciences, Shihezi University, Shihezi 832000, ChinaXinjiang Academy of Forestry Sciences, Urumqi 830000, ChinaXinjiang Academy of Forestry Sciences, Urumqi 830000, ChinaXinjiang Academy of Forestry Sciences, Urumqi 830000, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832000, ChinaFigs are an edible and medicinal plant rich in polyphenols and flavonoids with unique pharmacological effects. However, the mechanism of flavonoid synthesis in figs is not clear. In this study, fig fruits of six varieties were collected for RNA sequencing and UPLC-MS data collection. The results showed that a total of 39 differential metabolites were identified by targeted metabolomics, and their contents were determined by UPLC-MS. The clustered heat map analysis showed that most of the differential metabolites were highly accumulated in BRD and FY. A total of 62 flavonoid biosynthesis pathway genes were identified by transcriptome analysis, and <i>FcCHS</i>, <i>FcCHI</i>, <i>FcFLS</i>, <i>FcCYP</i>, and <i>FcDFR</i> were the key genes identified for the accumulation of flavonoids and flavonols in the dark-colored varieties. In addition, a total of 1671 transcription factor genes, mainly MYBs, bHLHs, and AP2/ERFs, were identified. This study will enrich the transcriptomic data of figs and provide some help in resolving the synthesis mechanism of fig flavonoids.https://www.mdpi.com/2079-7737/14/2/184figsflavonoidbiosynthesistranscriptomemetabolome |
| spellingShingle | Junting Sun Hadir Yishake Ming Wang Hao Zhang Jie Yan Integrated Transcriptome and Targeted Metabolome for Resolving Flavonoid Biosynthesis in Figs (<i>Ficus carica Linn</i>.) Biology figs flavonoid biosynthesis transcriptome metabolome |
| title | Integrated Transcriptome and Targeted Metabolome for Resolving Flavonoid Biosynthesis in Figs (<i>Ficus carica Linn</i>.) |
| title_full | Integrated Transcriptome and Targeted Metabolome for Resolving Flavonoid Biosynthesis in Figs (<i>Ficus carica Linn</i>.) |
| title_fullStr | Integrated Transcriptome and Targeted Metabolome for Resolving Flavonoid Biosynthesis in Figs (<i>Ficus carica Linn</i>.) |
| title_full_unstemmed | Integrated Transcriptome and Targeted Metabolome for Resolving Flavonoid Biosynthesis in Figs (<i>Ficus carica Linn</i>.) |
| title_short | Integrated Transcriptome and Targeted Metabolome for Resolving Flavonoid Biosynthesis in Figs (<i>Ficus carica Linn</i>.) |
| title_sort | integrated transcriptome and targeted metabolome for resolving flavonoid biosynthesis in figs i ficus carica linn i |
| topic | figs flavonoid biosynthesis transcriptome metabolome |
| url | https://www.mdpi.com/2079-7737/14/2/184 |
| work_keys_str_mv | AT juntingsun integratedtranscriptomeandtargetedmetabolomeforresolvingflavonoidbiosynthesisinfigsificuscaricalinni AT hadiryishake integratedtranscriptomeandtargetedmetabolomeforresolvingflavonoidbiosynthesisinfigsificuscaricalinni AT mingwang integratedtranscriptomeandtargetedmetabolomeforresolvingflavonoidbiosynthesisinfigsificuscaricalinni AT haozhang integratedtranscriptomeandtargetedmetabolomeforresolvingflavonoidbiosynthesisinfigsificuscaricalinni AT jieyan integratedtranscriptomeandtargetedmetabolomeforresolvingflavonoidbiosynthesisinfigsificuscaricalinni |