A Simple Separation Method of the Protein and Polystyrene Bead-Labeled Protein for Enhancing the Performance of Fluorescent Sensor
Dielectrophoresis- (DEP-) based separation method between a protein, amyloid beta 42, and polystyrene (PS) beads in different microholes was demonstrated for enhancement of performance for bead-based fluorescent sensor. An intensity of ∇E2 was relative to a diameter of a microhole, and the diameters...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/8461380 |
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| author | Hye Jin Kim Dong-Hoon Kang Seung-Hoon Yang Eunji Lee Taewon Ha Byung Chul Lee Youngbaek Kim Kyo Seon Hwang Hyun-Joon Shin Jinsik Kim |
| author_facet | Hye Jin Kim Dong-Hoon Kang Seung-Hoon Yang Eunji Lee Taewon Ha Byung Chul Lee Youngbaek Kim Kyo Seon Hwang Hyun-Joon Shin Jinsik Kim |
| author_sort | Hye Jin Kim |
| collection | DOAJ |
| description | Dielectrophoresis- (DEP-) based separation method between a protein, amyloid beta 42, and polystyrene (PS) beads in different microholes was demonstrated for enhancement of performance for bead-based fluorescent sensor. An intensity of ∇E2 was relative to a diameter of a microhole, and the diameters of two microholes for separation between the protein and PS beads were simulated to 3 μm and 15 μm, respectively. The microholes were fabricated by microelectromechanical systems (MEMS). The separation between the protein and the PS beads was demonstrated by comparing the average intensity of fluorescence (AIF) by each molecule. Relative AIF was measured in various applying voltage and time conditions, and the conditions for allocating the PS beads into 15 μm hole were optimized at 80 mV and 15 min, respectively. In the optimized condition, the relative AIF was observed approximately 4.908 ± 0.299. Finally, in 3 μm and 15 μm hole, the AIFs were approximately 3.143 and −1.346 by 2 nm of protein and about −2.515 and 4.211 by 30 nm of the PS beads, respectively. The results showed that 2 nm of the protein and 30 nm of PS beads were separated by DEP force in each microhole effectively, and that our method is applicable as a new method to verify an efficiency of the labeling for bead-based fluorescent sensor ∇E2. |
| format | Article |
| id | doaj-art-b54017a6736f48a4ac1bbadd1182c1e9 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-b54017a6736f48a4ac1bbadd1182c1e92025-02-03T01:20:41ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732018-01-01201810.1155/2018/84613808461380A Simple Separation Method of the Protein and Polystyrene Bead-Labeled Protein for Enhancing the Performance of Fluorescent SensorHye Jin Kim0Dong-Hoon Kang1Seung-Hoon Yang2Eunji Lee3Taewon Ha4Byung Chul Lee5Youngbaek Kim6Kyo Seon Hwang7Hyun-Joon Shin8Jinsik Kim9Department of Clinical Pharmacology, Kyung Hee University, Seoul 02447, Republic of KoreaCenter for Bionics, Korea Institute of Science and Technology, Seoul 02792, Republic of KoreaSystems Biotechnology Research Center, Korea Institute of Science and Technology, Gangneung 25451, Republic of KoreaDepartment of Medical Biotechnology, Dongguk University, Seoul 04620, Republic of KoreaCenter for Nano-Photonics Convergence Technology, Korea Institute of Industrial Technology (KITECH), Gwangju 61012, Republic of KoreaCenter for BioMicrosystems, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of KoreaCenter for Nano-Photonics Convergence Technology, Korea Institute of Industrial Technology (KITECH), Gwangju 61012, Republic of KoreaDepartment of Clinical Pharmacology, Kyung Hee University, Seoul 02447, Republic of KoreaCenter for Bionics, Korea Institute of Science and Technology, Seoul 02792, Republic of KoreaDepartment of Medical Biotechnology, Dongguk University, Seoul 04620, Republic of KoreaDielectrophoresis- (DEP-) based separation method between a protein, amyloid beta 42, and polystyrene (PS) beads in different microholes was demonstrated for enhancement of performance for bead-based fluorescent sensor. An intensity of ∇E2 was relative to a diameter of a microhole, and the diameters of two microholes for separation between the protein and PS beads were simulated to 3 μm and 15 μm, respectively. The microholes were fabricated by microelectromechanical systems (MEMS). The separation between the protein and the PS beads was demonstrated by comparing the average intensity of fluorescence (AIF) by each molecule. Relative AIF was measured in various applying voltage and time conditions, and the conditions for allocating the PS beads into 15 μm hole were optimized at 80 mV and 15 min, respectively. In the optimized condition, the relative AIF was observed approximately 4.908 ± 0.299. Finally, in 3 μm and 15 μm hole, the AIFs were approximately 3.143 and −1.346 by 2 nm of protein and about −2.515 and 4.211 by 30 nm of the PS beads, respectively. The results showed that 2 nm of the protein and 30 nm of PS beads were separated by DEP force in each microhole effectively, and that our method is applicable as a new method to verify an efficiency of the labeling for bead-based fluorescent sensor ∇E2.http://dx.doi.org/10.1155/2018/8461380 |
| spellingShingle | Hye Jin Kim Dong-Hoon Kang Seung-Hoon Yang Eunji Lee Taewon Ha Byung Chul Lee Youngbaek Kim Kyo Seon Hwang Hyun-Joon Shin Jinsik Kim A Simple Separation Method of the Protein and Polystyrene Bead-Labeled Protein for Enhancing the Performance of Fluorescent Sensor Journal of Analytical Methods in Chemistry |
| title | A Simple Separation Method of the Protein and Polystyrene Bead-Labeled Protein for Enhancing the Performance of Fluorescent Sensor |
| title_full | A Simple Separation Method of the Protein and Polystyrene Bead-Labeled Protein for Enhancing the Performance of Fluorescent Sensor |
| title_fullStr | A Simple Separation Method of the Protein and Polystyrene Bead-Labeled Protein for Enhancing the Performance of Fluorescent Sensor |
| title_full_unstemmed | A Simple Separation Method of the Protein and Polystyrene Bead-Labeled Protein for Enhancing the Performance of Fluorescent Sensor |
| title_short | A Simple Separation Method of the Protein and Polystyrene Bead-Labeled Protein for Enhancing the Performance of Fluorescent Sensor |
| title_sort | simple separation method of the protein and polystyrene bead labeled protein for enhancing the performance of fluorescent sensor |
| url | http://dx.doi.org/10.1155/2018/8461380 |
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