Failure to Detect XMRV-Specific Antibodies in the Plasma of CFS Patients Using Highly Sensitive Chemiluminescence Immunoassays
In 2009, Lombardi et al. reported their startling finding that the gammaretrovirus xenotropic murine leukemia virus-related retrovirus (XMRV) is present in 67% of blood samples of patients suffering from chronic fatigue syndrome (CFS), as opposed to only 3.7% of samples from healthy individuals. Ho...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Advances in Virology |
| Online Access: | http://dx.doi.org/10.1155/2011/854540 |
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| author | Brendan Oakes Xiaoxing Qiu Susan Levine John Hackett Brigitte T. Huber |
| author_facet | Brendan Oakes Xiaoxing Qiu Susan Levine John Hackett Brigitte T. Huber |
| author_sort | Brendan Oakes |
| collection | DOAJ |
| description | In 2009, Lombardi et al. reported their startling finding that the gammaretrovirus xenotropic murine leukemia virus-related retrovirus (XMRV) is present in 67% of blood samples of patients suffering from chronic fatigue syndrome (CFS), as opposed to only 3.7% of samples from healthy individuals. However, we and others could not confirm these results, using a nested PCR assay. An alternative to this highly sensitive, but contamination-prone, technique is to measure the serological response to XMRV. Thus, we tested the plasma samples from our cohorts of CFS patients and healthy controls for the presence of XMRV-specific antibodies. Using two novel chemiluminescence immunoassays (CMIAs), we show that none of our samples have any XMRV-reactive antibodies. Taken together with our previous findings, we conclude that XMRV is not present in any human individual tested by us, regardless of CFS or healthy control. |
| format | Article |
| id | doaj-art-b53555e5185c474fb174006627c7a2c8 |
| institution | OA Journals |
| issn | 1687-8639 1687-8647 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Virology |
| spelling | doaj-art-b53555e5185c474fb174006627c7a2c82025-08-20T02:23:23ZengWileyAdvances in Virology1687-86391687-86472011-01-01201110.1155/2011/854540854540Failure to Detect XMRV-Specific Antibodies in the Plasma of CFS Patients Using Highly Sensitive Chemiluminescence ImmunoassaysBrendan Oakes0Xiaoxing Qiu1Susan Levine2John Hackett3Brigitte T. Huber4Pathology Department, Tufts University School of Medicine, 150 Harrison Avenue, Boston, MA 02111, USAInfectious Diseases R&D, Abbott Diagnostics, 100 Abbott Park Road, Abbott Park, IL 60064, USAPrivate Practice, 115 East 72nd Street, New York, NY 10021, USAInfectious Diseases R&D, Abbott Diagnostics, 100 Abbott Park Road, Abbott Park, IL 60064, USAPathology Department, Tufts University School of Medicine, 150 Harrison Avenue, Boston, MA 02111, USAIn 2009, Lombardi et al. reported their startling finding that the gammaretrovirus xenotropic murine leukemia virus-related retrovirus (XMRV) is present in 67% of blood samples of patients suffering from chronic fatigue syndrome (CFS), as opposed to only 3.7% of samples from healthy individuals. However, we and others could not confirm these results, using a nested PCR assay. An alternative to this highly sensitive, but contamination-prone, technique is to measure the serological response to XMRV. Thus, we tested the plasma samples from our cohorts of CFS patients and healthy controls for the presence of XMRV-specific antibodies. Using two novel chemiluminescence immunoassays (CMIAs), we show that none of our samples have any XMRV-reactive antibodies. Taken together with our previous findings, we conclude that XMRV is not present in any human individual tested by us, regardless of CFS or healthy control.http://dx.doi.org/10.1155/2011/854540 |
| spellingShingle | Brendan Oakes Xiaoxing Qiu Susan Levine John Hackett Brigitte T. Huber Failure to Detect XMRV-Specific Antibodies in the Plasma of CFS Patients Using Highly Sensitive Chemiluminescence Immunoassays Advances in Virology |
| title | Failure to Detect XMRV-Specific Antibodies in the Plasma of CFS Patients Using Highly Sensitive Chemiluminescence Immunoassays |
| title_full | Failure to Detect XMRV-Specific Antibodies in the Plasma of CFS Patients Using Highly Sensitive Chemiluminescence Immunoassays |
| title_fullStr | Failure to Detect XMRV-Specific Antibodies in the Plasma of CFS Patients Using Highly Sensitive Chemiluminescence Immunoassays |
| title_full_unstemmed | Failure to Detect XMRV-Specific Antibodies in the Plasma of CFS Patients Using Highly Sensitive Chemiluminescence Immunoassays |
| title_short | Failure to Detect XMRV-Specific Antibodies in the Plasma of CFS Patients Using Highly Sensitive Chemiluminescence Immunoassays |
| title_sort | failure to detect xmrv specific antibodies in the plasma of cfs patients using highly sensitive chemiluminescence immunoassays |
| url | http://dx.doi.org/10.1155/2011/854540 |
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