Molecular Identification and Bioactivity Screening of Six Wild Strains of Morchella sextelata
This study aimed to identify high-quality Morchella strains with strong biological activity and stable fruiting characteristics. We employed single-gene and multi-gene identification techniques for molecular biological identification of six wild strains of Morchella and evaluated their functional tr...
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China Food Publishing Company
2025-07-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-13-008.pdf |
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| author | XIANG Yumeng, WU Qi, REN Haiwei, TIAN Yaqin, GUO Xiaopeng, LIU Qiankui, WANG Yayu |
| author_facet | XIANG Yumeng, WU Qi, REN Haiwei, TIAN Yaqin, GUO Xiaopeng, LIU Qiankui, WANG Yayu |
| author_sort | XIANG Yumeng, WU Qi, REN Haiwei, TIAN Yaqin, GUO Xiaopeng, LIU Qiankui, WANG Yayu |
| collection | DOAJ |
| description | This study aimed to identify high-quality Morchella strains with strong biological activity and stable fruiting characteristics. We employed single-gene and multi-gene identification techniques for molecular biological identification of six wild strains of Morchella and evaluated their functional traits by measuring various enzyme activities. The activities of antioxidant enzymes catalase (CAT), polyphenol oxidase (PPO) and peroxidase (POD) and malondialdehyde (MDA) content were assayed in liquid-cultured mycelia, and a quantitative assessment of these indicators was performed using the subordinate function method. As a result, we successfully identified an excellent strain suitable for artificial cultivation. Molecular identification confirmed that all six wild Morchella strains belonged to Morchella sextelata, under the Elata Clade group. Morphological observation revealed that the mycelia gradually changed from white to pale yellow, exhibiting dense arrangements with blunt ends and uneven diameters and showing localized spherical distribution characteristics, with a daily growth rate of 0.40–0.45 mm. Mating-type gene analysis indicated that strains LM1, LM3, and LM4 carried both MAT1-1 and MAT1-2 genes, suggesting potential for sexual reproduction. Compared with the two other strains, LM4 exhibited significantly higher PPO, POD, CAT, and 2,3,5-triphenyltetrazolium chloride (TTC) dehydrogenase activities and lower MDA content. The average subordinate function value of LM4 was the highest (0.964 7), highlighting its excellent biological activity and suitability for artificial cultivation. This study provides a novel approach for precise identification and efficient breeding of Morchella germplasm resources. |
| format | Article |
| id | doaj-art-b533d99d8428435ca04508a5a37e2ac8 |
| institution | DOAJ |
| issn | 1002-6630 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj-art-b533d99d8428435ca04508a5a37e2ac82025-08-20T03:12:32ZengChina Food Publishing CompanyShipin Kexue1002-66302025-07-014613867410.7506/spkx1002-6630-20250107-047Molecular Identification and Bioactivity Screening of Six Wild Strains of Morchella sextelataXIANG Yumeng, WU Qi, REN Haiwei, TIAN Yaqin, GUO Xiaopeng, LIU Qiankui, WANG Yayu0(1. School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China; 2. Gansu Provincial Food and Drug Resources Development and Biological Manufacturing Industry Technology Center, Lanzhou 730050, China; 3. Northwest Low Carbon Support Technology Provincial-Ministerial Collaborative Innovation Center, Lanzhou 730050, China; 4. Gansu Ruikang Modern Biotechnology Co. Ltd., Lanzhou 730050, China)This study aimed to identify high-quality Morchella strains with strong biological activity and stable fruiting characteristics. We employed single-gene and multi-gene identification techniques for molecular biological identification of six wild strains of Morchella and evaluated their functional traits by measuring various enzyme activities. The activities of antioxidant enzymes catalase (CAT), polyphenol oxidase (PPO) and peroxidase (POD) and malondialdehyde (MDA) content were assayed in liquid-cultured mycelia, and a quantitative assessment of these indicators was performed using the subordinate function method. As a result, we successfully identified an excellent strain suitable for artificial cultivation. Molecular identification confirmed that all six wild Morchella strains belonged to Morchella sextelata, under the Elata Clade group. Morphological observation revealed that the mycelia gradually changed from white to pale yellow, exhibiting dense arrangements with blunt ends and uneven diameters and showing localized spherical distribution characteristics, with a daily growth rate of 0.40–0.45 mm. Mating-type gene analysis indicated that strains LM1, LM3, and LM4 carried both MAT1-1 and MAT1-2 genes, suggesting potential for sexual reproduction. Compared with the two other strains, LM4 exhibited significantly higher PPO, POD, CAT, and 2,3,5-triphenyltetrazolium chloride (TTC) dehydrogenase activities and lower MDA content. The average subordinate function value of LM4 was the highest (0.964 7), highlighting its excellent biological activity and suitability for artificial cultivation. This study provides a novel approach for precise identification and efficient breeding of Morchella germplasm resources.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-13-008.pdfmorchella esculenta; strain identification; mating-type genes; biomass; antioxidant enzyme activity |
| spellingShingle | XIANG Yumeng, WU Qi, REN Haiwei, TIAN Yaqin, GUO Xiaopeng, LIU Qiankui, WANG Yayu Molecular Identification and Bioactivity Screening of Six Wild Strains of Morchella sextelata Shipin Kexue morchella esculenta; strain identification; mating-type genes; biomass; antioxidant enzyme activity |
| title | Molecular Identification and Bioactivity Screening of Six Wild Strains of Morchella sextelata |
| title_full | Molecular Identification and Bioactivity Screening of Six Wild Strains of Morchella sextelata |
| title_fullStr | Molecular Identification and Bioactivity Screening of Six Wild Strains of Morchella sextelata |
| title_full_unstemmed | Molecular Identification and Bioactivity Screening of Six Wild Strains of Morchella sextelata |
| title_short | Molecular Identification and Bioactivity Screening of Six Wild Strains of Morchella sextelata |
| title_sort | molecular identification and bioactivity screening of six wild strains of morchella sextelata |
| topic | morchella esculenta; strain identification; mating-type genes; biomass; antioxidant enzyme activity |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-13-008.pdf |
| work_keys_str_mv | AT xiangyumengwuqirenhaiweitianyaqinguoxiaopengliuqiankuiwangyayu molecularidentificationandbioactivityscreeningofsixwildstrainsofmorchellasextelata |