Assembly of oleosin during efficient extraction: Altering the sequence of defatting solvents
During the extraction of membrane proteins from oil bodies (OBs), organic solvents dissolve the lipid core and precipitate proteins through solvent stress. Here the effects of solvent type and defatting sequence on the composition and structure of membrane proteins were investigated via SDS-PAGE, FT...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
|
| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157524009106 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823856766572560384 |
|---|---|
| author | Yu Li Yuqian Qiao Yuxuan Zhu Wangyang Shen Weiping Jin Dengfeng Peng Qingrong Huang |
| author_facet | Yu Li Yuqian Qiao Yuxuan Zhu Wangyang Shen Weiping Jin Dengfeng Peng Qingrong Huang |
| author_sort | Yu Li |
| collection | DOAJ |
| description | During the extraction of membrane proteins from oil bodies (OBs), organic solvents dissolve the lipid core and precipitate proteins through solvent stress. Here the effects of solvent type and defatting sequence on the composition and structure of membrane proteins were investigated via SDS-PAGE, FTIR, and SEM-EDS. High purity oleosin (86 %) was obtained by treatment first with a Floch solution and then with cold acetone and petroleum ether after twice washing OBs with urea. The 3D spatial structure of oleosin was predicted using AlphaFold 2, revealing that the secondary structure of oleosin was dominated by α-helices (>60 %). Oleosin consisted of two district types, with oleosin-H (16–17 kDa) being the part of the molecule with limited water solubility, while oleosin-L (13–14 kDa) constituted the non-soluble part. The results provided a technical means of efficient extraction of Camellia oleosins and selective separation of oleosin-L and oleosin-H. |
| format | Article |
| id | doaj-art-b5319778fdd64214834a2aff1f1bdb33 |
| institution | Kabale University |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-b5319778fdd64214834a2aff1f1bdb332025-02-12T05:31:46ZengElsevierFood Chemistry: X2590-15752025-01-0125102022Assembly of oleosin during efficient extraction: Altering the sequence of defatting solventsYu Li0Yuqian Qiao1Yuxuan Zhu2Wangyang Shen3Weiping Jin4Dengfeng Peng5Qingrong Huang6School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430023, PR ChinaSchool of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430023, PR ChinaSchool of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430023, PR ChinaSchool of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430023, PR ChinaSchool of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430023, PR China; Corresponding author.Key Laboratory of Oilseeds Processing, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, PR ChinaDepartment of Food Science, Rutgers University, 65 Dudley Road, New Brunswick, NJ 08901, USADuring the extraction of membrane proteins from oil bodies (OBs), organic solvents dissolve the lipid core and precipitate proteins through solvent stress. Here the effects of solvent type and defatting sequence on the composition and structure of membrane proteins were investigated via SDS-PAGE, FTIR, and SEM-EDS. High purity oleosin (86 %) was obtained by treatment first with a Floch solution and then with cold acetone and petroleum ether after twice washing OBs with urea. The 3D spatial structure of oleosin was predicted using AlphaFold 2, revealing that the secondary structure of oleosin was dominated by α-helices (>60 %). Oleosin consisted of two district types, with oleosin-H (16–17 kDa) being the part of the molecule with limited water solubility, while oleosin-L (13–14 kDa) constituted the non-soluble part. The results provided a technical means of efficient extraction of Camellia oleosins and selective separation of oleosin-L and oleosin-H.http://www.sciencedirect.com/science/article/pii/S2590157524009106OleosinProtein assemblyOrganic solventsExtractionOil bodies |
| spellingShingle | Yu Li Yuqian Qiao Yuxuan Zhu Wangyang Shen Weiping Jin Dengfeng Peng Qingrong Huang Assembly of oleosin during efficient extraction: Altering the sequence of defatting solvents Food Chemistry: X Oleosin Protein assembly Organic solvents Extraction Oil bodies |
| title | Assembly of oleosin during efficient extraction: Altering the sequence of defatting solvents |
| title_full | Assembly of oleosin during efficient extraction: Altering the sequence of defatting solvents |
| title_fullStr | Assembly of oleosin during efficient extraction: Altering the sequence of defatting solvents |
| title_full_unstemmed | Assembly of oleosin during efficient extraction: Altering the sequence of defatting solvents |
| title_short | Assembly of oleosin during efficient extraction: Altering the sequence of defatting solvents |
| title_sort | assembly of oleosin during efficient extraction altering the sequence of defatting solvents |
| topic | Oleosin Protein assembly Organic solvents Extraction Oil bodies |
| url | http://www.sciencedirect.com/science/article/pii/S2590157524009106 |
| work_keys_str_mv | AT yuli assemblyofoleosinduringefficientextractionalteringthesequenceofdefattingsolvents AT yuqianqiao assemblyofoleosinduringefficientextractionalteringthesequenceofdefattingsolvents AT yuxuanzhu assemblyofoleosinduringefficientextractionalteringthesequenceofdefattingsolvents AT wangyangshen assemblyofoleosinduringefficientextractionalteringthesequenceofdefattingsolvents AT weipingjin assemblyofoleosinduringefficientextractionalteringthesequenceofdefattingsolvents AT dengfengpeng assemblyofoleosinduringefficientextractionalteringthesequenceofdefattingsolvents AT qingronghuang assemblyofoleosinduringefficientextractionalteringthesequenceofdefattingsolvents |