Cytoskeletal Interactions at the Nuclear Envelope Mediated by Nesprins
Nesprin-1 is a giant tail-anchored nuclear envelope protein composed of an N-terminal F-actin binding domain, a long linker region formed by multiple spectrin repeats and a C-terminal transmembrane domain. Based on this structure, it connects the nucleus to the actin cytoskeleton. Earlier reports ha...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | International Journal of Cell Biology |
| Online Access: | http://dx.doi.org/10.1155/2012/736524 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832562634569809920 |
|---|---|
| author | Surayya Taranum Ilknur Sur Rolf Müller Wenshu Lu R. N. Rashmi Martina Munck Sascha Neumann Iakowos Karakesisoglou Angelika A. Noegel |
| author_facet | Surayya Taranum Ilknur Sur Rolf Müller Wenshu Lu R. N. Rashmi Martina Munck Sascha Neumann Iakowos Karakesisoglou Angelika A. Noegel |
| author_sort | Surayya Taranum |
| collection | DOAJ |
| description | Nesprin-1 is a giant tail-anchored nuclear envelope protein composed of an N-terminal F-actin binding domain, a long linker region formed by multiple spectrin repeats and a C-terminal transmembrane domain. Based on this structure, it connects the nucleus to the actin cytoskeleton. Earlier reports had shown that Nesprin-1 binds to nuclear envelope proteins emerin and lamin through C-terminal spectrin repeats. These repeats can also self-associate. We focus on the N-terminal Nesprin-1 sequences and show that they interact with Nesprin-3, a further member of the Nesprin family, which connects the nucleus to the intermediate filament network. We show that upon ectopic expression of Nesprin-3 in COS7 cells, which are nearly devoid of Nesprin-3 in vitro, vimentin filaments are recruited to the nucleus and provide evidence for an F-actin interaction of Nesprin-3 in vitro. We propose that Nesprins through interactions amongst themselves and amongst the various Nesprins form a network around the nucleus and connect the nucleus to several cytoskeletal networks of the cell. |
| format | Article |
| id | doaj-art-b52ca205ca22497a9109197aadbd6fed |
| institution | Kabale University |
| issn | 1687-8876 1687-8884 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Cell Biology |
| spelling | doaj-art-b52ca205ca22497a9109197aadbd6fed2025-02-03T01:22:11ZengWileyInternational Journal of Cell Biology1687-88761687-88842012-01-01201210.1155/2012/736524736524Cytoskeletal Interactions at the Nuclear Envelope Mediated by NesprinsSurayya Taranum0Ilknur Sur1Rolf Müller2Wenshu Lu3R. N. Rashmi4Martina Munck5Sascha Neumann6Iakowos Karakesisoglou7Angelika A. Noegel8Institute of Biochemistry I, Medical Faculty, University of Cologne, Joseph-Stelzmann-Strasse 52, 50931 Cologne, GermanyInstitute of Biochemistry I, Medical Faculty, University of Cologne, Joseph-Stelzmann-Strasse 52, 50931 Cologne, GermanyInstitute of Biochemistry I, Medical Faculty, University of Cologne, Joseph-Stelzmann-Strasse 52, 50931 Cologne, GermanyInstitute of Biochemistry I, Medical Faculty, University of Cologne, Joseph-Stelzmann-Strasse 52, 50931 Cologne, GermanyInstitute of Biochemistry I, Medical Faculty, University of Cologne, Joseph-Stelzmann-Strasse 52, 50931 Cologne, GermanyInstitute of Biochemistry I, Medical Faculty, University of Cologne, Joseph-Stelzmann-Strasse 52, 50931 Cologne, GermanyInstitute of Biochemistry I, Medical Faculty, University of Cologne, Joseph-Stelzmann-Strasse 52, 50931 Cologne, GermanyDepartment of Biological Sciences, School of Biological and Biomedical Sciences, University of Durham, Durham DH1 3LE, UKInstitute of Biochemistry I, Medical Faculty, University of Cologne, Joseph-Stelzmann-Strasse 52, 50931 Cologne, GermanyNesprin-1 is a giant tail-anchored nuclear envelope protein composed of an N-terminal F-actin binding domain, a long linker region formed by multiple spectrin repeats and a C-terminal transmembrane domain. Based on this structure, it connects the nucleus to the actin cytoskeleton. Earlier reports had shown that Nesprin-1 binds to nuclear envelope proteins emerin and lamin through C-terminal spectrin repeats. These repeats can also self-associate. We focus on the N-terminal Nesprin-1 sequences and show that they interact with Nesprin-3, a further member of the Nesprin family, which connects the nucleus to the intermediate filament network. We show that upon ectopic expression of Nesprin-3 in COS7 cells, which are nearly devoid of Nesprin-3 in vitro, vimentin filaments are recruited to the nucleus and provide evidence for an F-actin interaction of Nesprin-3 in vitro. We propose that Nesprins through interactions amongst themselves and amongst the various Nesprins form a network around the nucleus and connect the nucleus to several cytoskeletal networks of the cell.http://dx.doi.org/10.1155/2012/736524 |
| spellingShingle | Surayya Taranum Ilknur Sur Rolf Müller Wenshu Lu R. N. Rashmi Martina Munck Sascha Neumann Iakowos Karakesisoglou Angelika A. Noegel Cytoskeletal Interactions at the Nuclear Envelope Mediated by Nesprins International Journal of Cell Biology |
| title | Cytoskeletal Interactions at the Nuclear Envelope Mediated by Nesprins |
| title_full | Cytoskeletal Interactions at the Nuclear Envelope Mediated by Nesprins |
| title_fullStr | Cytoskeletal Interactions at the Nuclear Envelope Mediated by Nesprins |
| title_full_unstemmed | Cytoskeletal Interactions at the Nuclear Envelope Mediated by Nesprins |
| title_short | Cytoskeletal Interactions at the Nuclear Envelope Mediated by Nesprins |
| title_sort | cytoskeletal interactions at the nuclear envelope mediated by nesprins |
| url | http://dx.doi.org/10.1155/2012/736524 |
| work_keys_str_mv | AT surayyataranum cytoskeletalinteractionsatthenuclearenvelopemediatedbynesprins AT ilknursur cytoskeletalinteractionsatthenuclearenvelopemediatedbynesprins AT rolfmuller cytoskeletalinteractionsatthenuclearenvelopemediatedbynesprins AT wenshulu cytoskeletalinteractionsatthenuclearenvelopemediatedbynesprins AT rnrashmi cytoskeletalinteractionsatthenuclearenvelopemediatedbynesprins AT martinamunck cytoskeletalinteractionsatthenuclearenvelopemediatedbynesprins AT saschaneumann cytoskeletalinteractionsatthenuclearenvelopemediatedbynesprins AT iakowoskarakesisoglou cytoskeletalinteractionsatthenuclearenvelopemediatedbynesprins AT angelikaanoegel cytoskeletalinteractionsatthenuclearenvelopemediatedbynesprins |