Stability of Epidoxorubicin Hydrochloride in Aqueous Solutions: Experimental and Theoretical Studies

The first-order degradation kinetics of epidoxorubicin were investigated as a function of pH, temperature, and buffers concentrations. The degradation was followed by HPLC. Buffer catalysis was observed in acetate and phosphate buffers. The pH-rate profiles were obtained at 333, 343, 353, and 363 K....

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Main Authors: Agnieszka Sobczak, Monika A. Lesniewska-Kowiel, Izabela Muszalska, Artur Firlej, Judyta Cielecka-Piontek, Szymon Tomczak, Bolesław Barszcz, Irena Oszczapowicz, Anna Jelińska
Format: Article
Language:English
Published: Wiley 2017-01-01
Series:Journal of Chemistry
Online Access:http://dx.doi.org/10.1155/2017/8107140
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author Agnieszka Sobczak
Monika A. Lesniewska-Kowiel
Izabela Muszalska
Artur Firlej
Judyta Cielecka-Piontek
Szymon Tomczak
Bolesław Barszcz
Irena Oszczapowicz
Anna Jelińska
author_facet Agnieszka Sobczak
Monika A. Lesniewska-Kowiel
Izabela Muszalska
Artur Firlej
Judyta Cielecka-Piontek
Szymon Tomczak
Bolesław Barszcz
Irena Oszczapowicz
Anna Jelińska
author_sort Agnieszka Sobczak
collection DOAJ
description The first-order degradation kinetics of epidoxorubicin were investigated as a function of pH, temperature, and buffers concentrations. The degradation was followed by HPLC. Buffer catalysis was observed in acetate and phosphate buffers. The pH-rate profiles were obtained at 333, 343, 353, and 363 K. The pH-rate expression was kpH=k1×aH+×f1+k2×f1+k3×f2+(k4×f2+k5×f3)×aOH-, where k1, k4, and k5 are the second-order rate constants (mol−1 L s−1) for hydrogen ion activity and for hydroxyl ion activity, respectively, and k2 and k3 are the first-order constants (s−1) for spontaneous reaction under the influence of water. Epidoxorubicin demonstrates the greatest stability in the pH range 3–5. The electrostatic molecular potential orbitals HOMO-LUMO were also defined in order to determine the cause of the reactivity of particular epidoxorubicin molecule domains in solutions with various pH values.
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publishDate 2017-01-01
publisher Wiley
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spelling doaj-art-b5274e4a3057440ba3bdc515aba480fa2025-08-20T02:23:30ZengWileyJournal of Chemistry2090-90632090-90712017-01-01201710.1155/2017/81071408107140Stability of Epidoxorubicin Hydrochloride in Aqueous Solutions: Experimental and Theoretical StudiesAgnieszka Sobczak0Monika A. Lesniewska-Kowiel1Izabela Muszalska2Artur Firlej3Judyta Cielecka-Piontek4Szymon Tomczak5Bolesław Barszcz6Irena Oszczapowicz7Anna Jelińska8Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, PolandDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, PolandDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, PolandDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, PolandDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, PolandDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, PolandInstitute of Molecular Physics Polish Academy of Sciences, Smoluchowskiego 17, 60-179 Poznań, PolandDepartment of Modified Antibiotics, Institute of Biotechnology and Antibiotics, Starościńska 5, 02-515 Warszawa, PolandDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780 Poznań, PolandThe first-order degradation kinetics of epidoxorubicin were investigated as a function of pH, temperature, and buffers concentrations. The degradation was followed by HPLC. Buffer catalysis was observed in acetate and phosphate buffers. The pH-rate profiles were obtained at 333, 343, 353, and 363 K. The pH-rate expression was kpH=k1×aH+×f1+k2×f1+k3×f2+(k4×f2+k5×f3)×aOH-, where k1, k4, and k5 are the second-order rate constants (mol−1 L s−1) for hydrogen ion activity and for hydroxyl ion activity, respectively, and k2 and k3 are the first-order constants (s−1) for spontaneous reaction under the influence of water. Epidoxorubicin demonstrates the greatest stability in the pH range 3–5. The electrostatic molecular potential orbitals HOMO-LUMO were also defined in order to determine the cause of the reactivity of particular epidoxorubicin molecule domains in solutions with various pH values.http://dx.doi.org/10.1155/2017/8107140
spellingShingle Agnieszka Sobczak
Monika A. Lesniewska-Kowiel
Izabela Muszalska
Artur Firlej
Judyta Cielecka-Piontek
Szymon Tomczak
Bolesław Barszcz
Irena Oszczapowicz
Anna Jelińska
Stability of Epidoxorubicin Hydrochloride in Aqueous Solutions: Experimental and Theoretical Studies
Journal of Chemistry
title Stability of Epidoxorubicin Hydrochloride in Aqueous Solutions: Experimental and Theoretical Studies
title_full Stability of Epidoxorubicin Hydrochloride in Aqueous Solutions: Experimental and Theoretical Studies
title_fullStr Stability of Epidoxorubicin Hydrochloride in Aqueous Solutions: Experimental and Theoretical Studies
title_full_unstemmed Stability of Epidoxorubicin Hydrochloride in Aqueous Solutions: Experimental and Theoretical Studies
title_short Stability of Epidoxorubicin Hydrochloride in Aqueous Solutions: Experimental and Theoretical Studies
title_sort stability of epidoxorubicin hydrochloride in aqueous solutions experimental and theoretical studies
url http://dx.doi.org/10.1155/2017/8107140
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