Effect of different pretreatments on egyptian sugar-cane bagasse saccharification and bioethanol production
Sugar-cane processing generates large amount of bagasse. Disposal of bagasse is critical for both agricultural profitability and environmental protection. Sugar-cane bagasse is a renewable resource that can be used to produce ethanol. In this study, twelve microbial isolates, five bacteria, four yea...
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Egyptian Petroleum Research Institute
2013-06-01
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| Series: | Egyptian Journal of Petroleum |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S111006211200058X |
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| author | Mervate A. Abo-State Ahmed M.E. Ragab Nour Sh. EL-Gendy Laila A. Farahat Hekmat R. Madian |
| author_facet | Mervate A. Abo-State Ahmed M.E. Ragab Nour Sh. EL-Gendy Laila A. Farahat Hekmat R. Madian |
| author_sort | Mervate A. Abo-State |
| collection | DOAJ |
| description | Sugar-cane processing generates large amount of bagasse. Disposal of bagasse is critical for both agricultural profitability and environmental protection. Sugar-cane bagasse is a renewable resource that can be used to produce ethanol.
In this study, twelve microbial isolates, five bacteria, four yeasts and three filamentous fungi were isolated from sugar-cane bagasse. Bacterial and yeast isolates were selected for their ability to utilize different sugars and cellulose. Chipped and ground bagasse was subjected to different pretreatment methods; physically through steam treatment by autoclaving at 121 °C and 1.5 bar for 20 min and/or different doses of gamma γ irradiation (50 and 70 Mrad). Autoclaved pretreated bagasse was further biologically treated through the solid state fermentation process by different fungal isolates; F-66, F-94 and F-98 producing maximum total reducing sugars of 18.4., 26.1 and 20.4 g/L, respectively.
Separate biological hydrolysis and fermentation (SHF) process for bagasse was done by the two selected fungal isolates; Trichoderma viride F-94 and Aspergillus terreus F-98 and the two yeast isolates identified as Candida tropicalis Y-26 and Saccharomyces cerevisiae Y-39. SHF processes by F-94 and Y-26 produced 226 kg of ethanol/ton bagasse while that of F-98 and Y-39 produced 185 kg of ethanol/ton bagasse. |
| format | Article |
| id | doaj-art-b51d1be378e04421b09833f9e8a172d6 |
| institution | DOAJ |
| issn | 1110-0621 |
| language | English |
| publishDate | 2013-06-01 |
| publisher | Egyptian Petroleum Research Institute |
| record_format | Article |
| series | Egyptian Journal of Petroleum |
| spelling | doaj-art-b51d1be378e04421b09833f9e8a172d62025-08-20T03:04:43ZengEgyptian Petroleum Research InstituteEgyptian Journal of Petroleum1110-06212013-06-0122116116710.1016/j.ejpe.2012.09.007Effect of different pretreatments on egyptian sugar-cane bagasse saccharification and bioethanol productionMervate A. Abo-State0Ahmed M.E. Ragab1Nour Sh. EL-Gendy2Laila A. Farahat3Hekmat R. Madian4National Center for Radiation Research and Technology (NCRRT), Nasr City, Cairo, EgyptFaculty of Science (Girls Branch), Al-Azhar University, Cairo, EgyptEgyptian Petroleum Research Institute (EPRI), Nasr City, Cairo, EgyptEgyptian Petroleum Research Institute (EPRI), Nasr City, Cairo, EgyptEgyptian Petroleum Research Institute (EPRI), Nasr City, Cairo, EgyptSugar-cane processing generates large amount of bagasse. Disposal of bagasse is critical for both agricultural profitability and environmental protection. Sugar-cane bagasse is a renewable resource that can be used to produce ethanol. In this study, twelve microbial isolates, five bacteria, four yeasts and three filamentous fungi were isolated from sugar-cane bagasse. Bacterial and yeast isolates were selected for their ability to utilize different sugars and cellulose. Chipped and ground bagasse was subjected to different pretreatment methods; physically through steam treatment by autoclaving at 121 °C and 1.5 bar for 20 min and/or different doses of gamma γ irradiation (50 and 70 Mrad). Autoclaved pretreated bagasse was further biologically treated through the solid state fermentation process by different fungal isolates; F-66, F-94 and F-98 producing maximum total reducing sugars of 18.4., 26.1 and 20.4 g/L, respectively. Separate biological hydrolysis and fermentation (SHF) process for bagasse was done by the two selected fungal isolates; Trichoderma viride F-94 and Aspergillus terreus F-98 and the two yeast isolates identified as Candida tropicalis Y-26 and Saccharomyces cerevisiae Y-39. SHF processes by F-94 and Y-26 produced 226 kg of ethanol/ton bagasse while that of F-98 and Y-39 produced 185 kg of ethanol/ton bagasse.http://www.sciencedirect.com/science/article/pii/S111006211200058XSugar-cane bagasseTrichoderma virideAspergillus terreusSaccharomyces cerevisiaeCandida tropicalisBioethanolSeparate hydrolysis fermentation |
| spellingShingle | Mervate A. Abo-State Ahmed M.E. Ragab Nour Sh. EL-Gendy Laila A. Farahat Hekmat R. Madian Effect of different pretreatments on egyptian sugar-cane bagasse saccharification and bioethanol production Egyptian Journal of Petroleum Sugar-cane bagasse Trichoderma viride Aspergillus terreus Saccharomyces cerevisiae Candida tropicalis Bioethanol Separate hydrolysis fermentation |
| title | Effect of different pretreatments on egyptian sugar-cane bagasse saccharification and bioethanol production |
| title_full | Effect of different pretreatments on egyptian sugar-cane bagasse saccharification and bioethanol production |
| title_fullStr | Effect of different pretreatments on egyptian sugar-cane bagasse saccharification and bioethanol production |
| title_full_unstemmed | Effect of different pretreatments on egyptian sugar-cane bagasse saccharification and bioethanol production |
| title_short | Effect of different pretreatments on egyptian sugar-cane bagasse saccharification and bioethanol production |
| title_sort | effect of different pretreatments on egyptian sugar cane bagasse saccharification and bioethanol production |
| topic | Sugar-cane bagasse Trichoderma viride Aspergillus terreus Saccharomyces cerevisiae Candida tropicalis Bioethanol Separate hydrolysis fermentation |
| url | http://www.sciencedirect.com/science/article/pii/S111006211200058X |
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