Effect of bispecific recombinant oncolytic adenovirus carrying apoptin on apoptosis of MCF-7 cells
ObjectiveIn this study, breast cancer cell line MCF-7 was infected with recombinant oncolytic adenovirus Ad-VT expressing apopsin protein, and its anti-tumour pathway was detected to determine its possible anti-tumour signalling pathway.MethodIn this study, the inhibitory effect of recombinant oncol...
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Frontiers Media S.A.
2025-05-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1530583/full |
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| author | Shuang Chen Shuang Chen Wenyao Li Wenyao Li Xunzhe Yin Xunzhe Yin Cheng Hu Yiquan Li Xiaotong Shao Xiao Li Ningyi Jin |
| author_facet | Shuang Chen Shuang Chen Wenyao Li Wenyao Li Xunzhe Yin Xunzhe Yin Cheng Hu Yiquan Li Xiaotong Shao Xiao Li Ningyi Jin |
| author_sort | Shuang Chen |
| collection | DOAJ |
| description | ObjectiveIn this study, breast cancer cell line MCF-7 was infected with recombinant oncolytic adenovirus Ad-VT expressing apopsin protein, and its anti-tumour pathway was detected to determine its possible anti-tumour signalling pathway.MethodIn this study, the inhibitory effect of recombinant oncolytic adenovirus Ad-VT on breast cancer cells was investigated through cell activity experiment and establishment of tumour bearing model in mice. Subsequently, in order to determine the apoptosis-inducing effect of recombinant oncolytic adenovirus on breast cancer cells, the effects of three recombinant oncolytic adenovirus on the apoptosis-inducing level of breast cancer cells were further analysed by Annexin V-FITC/PI detection, Hoechst staining, JC-1 staining and transmission electron microscopy. Then the differentially expressed proteins associated with apoptosis and possible signalling pathways were identified by proteomics and WB experiments.ResultsIn vivo and in vitro experiments showed that recombinant oncolytic adenovirus Ad-VT expressing apoptosis protein could induce apoptosis and inhibit the growth of MCF-7 cells. Proteomic analysis showed that differential genes were enriched in mTOR, MAPK and other pathways after Ad-VT infection of breast cancer cells, and the expression of S6K genes related to mTOR pathway was significantly increased in differential gene analysis, subsequently, the high expression of phosphorylated mTOR and S6K proteins was also determined by WB experiment, suggesting that Ad-VT may regulate the apoptosis of breast cancer cells through mTOR/S6K signalling.ConclusionAd-VT can significantly increase the apoptosis level of breast cancer cells, which may be induced by the mTOR/S6K signalling pathway. The results of this study provide a theoretical basis for the development of anti-tumour drugs based on Ad-VT in the future. |
| format | Article |
| id | doaj-art-b50a8527ba5440fd879bbd4e9cebd07a |
| institution | DOAJ |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-b50a8527ba5440fd879bbd4e9cebd07a2025-08-20T03:09:44ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-05-011610.3389/fimmu.2025.15305831530583Effect of bispecific recombinant oncolytic adenovirus carrying apoptin on apoptosis of MCF-7 cellsShuang Chen0Shuang Chen1Wenyao Li2Wenyao Li3Xunzhe Yin4Xunzhe Yin5Cheng Hu6Yiquan Li7Xiaotong Shao8Xiao Li9Ningyi Jin10School of Laboratory Medicine, Jilin Medical University, Jilin, ChinaInstitute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, ChinaSchool of Laboratory Medicine, Jilin Medical University, Jilin, ChinaCollege of Medicine, Dalian University, Dalian, ChinaSchool of Laboratory Medicine, Jilin Medical University, Jilin, ChinaChangchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, ChinaSchool of Laboratory Medicine, Jilin Medical University, Jilin, ChinaInstitute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, ChinaSchool of Laboratory Medicine, Jilin Medical University, Jilin, ChinaInstitute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, ChinaSchool of Laboratory Medicine, Jilin Medical University, Jilin, ChinaObjectiveIn this study, breast cancer cell line MCF-7 was infected with recombinant oncolytic adenovirus Ad-VT expressing apopsin protein, and its anti-tumour pathway was detected to determine its possible anti-tumour signalling pathway.MethodIn this study, the inhibitory effect of recombinant oncolytic adenovirus Ad-VT on breast cancer cells was investigated through cell activity experiment and establishment of tumour bearing model in mice. Subsequently, in order to determine the apoptosis-inducing effect of recombinant oncolytic adenovirus on breast cancer cells, the effects of three recombinant oncolytic adenovirus on the apoptosis-inducing level of breast cancer cells were further analysed by Annexin V-FITC/PI detection, Hoechst staining, JC-1 staining and transmission electron microscopy. Then the differentially expressed proteins associated with apoptosis and possible signalling pathways were identified by proteomics and WB experiments.ResultsIn vivo and in vitro experiments showed that recombinant oncolytic adenovirus Ad-VT expressing apoptosis protein could induce apoptosis and inhibit the growth of MCF-7 cells. Proteomic analysis showed that differential genes were enriched in mTOR, MAPK and other pathways after Ad-VT infection of breast cancer cells, and the expression of S6K genes related to mTOR pathway was significantly increased in differential gene analysis, subsequently, the high expression of phosphorylated mTOR and S6K proteins was also determined by WB experiment, suggesting that Ad-VT may regulate the apoptosis of breast cancer cells through mTOR/S6K signalling.ConclusionAd-VT can significantly increase the apoptosis level of breast cancer cells, which may be induced by the mTOR/S6K signalling pathway. The results of this study provide a theoretical basis for the development of anti-tumour drugs based on Ad-VT in the future.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1530583/fullMCF-7 cellsapoptinproteomicsapoptosisoncolytic adenovirus |
| spellingShingle | Shuang Chen Shuang Chen Wenyao Li Wenyao Li Xunzhe Yin Xunzhe Yin Cheng Hu Yiquan Li Xiaotong Shao Xiao Li Ningyi Jin Effect of bispecific recombinant oncolytic adenovirus carrying apoptin on apoptosis of MCF-7 cells Frontiers in Immunology MCF-7 cells apoptin proteomics apoptosis oncolytic adenovirus |
| title | Effect of bispecific recombinant oncolytic adenovirus carrying apoptin on apoptosis of MCF-7 cells |
| title_full | Effect of bispecific recombinant oncolytic adenovirus carrying apoptin on apoptosis of MCF-7 cells |
| title_fullStr | Effect of bispecific recombinant oncolytic adenovirus carrying apoptin on apoptosis of MCF-7 cells |
| title_full_unstemmed | Effect of bispecific recombinant oncolytic adenovirus carrying apoptin on apoptosis of MCF-7 cells |
| title_short | Effect of bispecific recombinant oncolytic adenovirus carrying apoptin on apoptosis of MCF-7 cells |
| title_sort | effect of bispecific recombinant oncolytic adenovirus carrying apoptin on apoptosis of mcf 7 cells |
| topic | MCF-7 cells apoptin proteomics apoptosis oncolytic adenovirus |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1530583/full |
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