Probable New Species of Bacteria of the Genus <i>Pseudomonas</i> Accelerates and Enhances the Destruction of Perfluorocarboxylic Acids
Bacteria of the genus <i>Pseudomonas</i> are the most studied microorganisms that biodegrade persistent perfluoroorganic pollutants, and the research of their application for the remediation of environmental sites using biotechnological approaches remains relevant. The aim of this study...
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2024-12-01
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| author | Sergey Chetverikov Gaisar Hkudaigulov Danil Sharipov Sergey Starikov |
| author_facet | Sergey Chetverikov Gaisar Hkudaigulov Danil Sharipov Sergey Starikov |
| author_sort | Sergey Chetverikov |
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| description | Bacteria of the genus <i>Pseudomonas</i> are the most studied microorganisms that biodegrade persistent perfluoroorganic pollutants, and the research of their application for the remediation of environmental sites using biotechnological approaches remains relevant. The aim of this study was to investigate the ability of a known destructor of perfluorooctane sulfonic acid from the genus <i>Pseudomonas</i> to accelerate and enhance the destruction of long-chain perfluorocarboxylic acids (PFCAs), specifically perfluorooctanoic acid and perfluorononanoic acid, in water and soil in association with the strain <i>P</i>. <i>mosselii</i> 5(3), which has previously confirmed genetic potential for the degrading of PFCAs. The complete genome (5.86 million base pairs) of the strain 2,4-D, probably belonging to a new species of <i>Pseudomonas</i>, was sequenced, assembled, and analyzed. The genomes of both strains contain genes involved in the defluorination of fluorinated compounds, including haloacetate dehalogenase H-1 (<i>dehH1</i>) and haloalkane dehalogenase (<i>dhaA</i>). The strain 2,4-D also has a multicomponent enzyme system consisting of a dioxygenase component, an electron carrier, and 2-halobenzoate 1,2-dioxygenase (CbdA) with a preference for fluorides. The strain 2,4-D was able to defluorinate PFCAs in an aqueous cultivation system within 7 days, using them as the sole source of carbon and energy and converting them to perfluorheptanoic acid. It assisted strain 5(3) to convert PFCAs to perfluoropentanoic acid, accelerating the process by 24 h. In a model experiment for the bioaugmentation of microorganisms in artificially contaminated soil, the degradation of PFCAs by the association of pseudomonads also occurred faster and deeper than by the individual strains, achieving a degree of biodestruction of 75% over 60 days, with the perfluoropentanoic acid as the main metabolite. These results are of great importance for the development of methods for the biological recultivation of fluorinated organic pollutants for environmental protection and for understanding the fundamental mechanisms of bacterial interactions with these compounds. |
| format | Article |
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| institution | DOAJ |
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| publishDate | 2024-12-01 |
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| spelling | doaj-art-b50a7809e8484c1d8e2c203b250c3cf72025-08-20T02:57:21ZengMDPI AGToxics2305-63042024-12-01121293010.3390/toxics12120930Probable New Species of Bacteria of the Genus <i>Pseudomonas</i> Accelerates and Enhances the Destruction of Perfluorocarboxylic AcidsSergey Chetverikov0Gaisar Hkudaigulov1Danil Sharipov2Sergey Starikov3Ufa Institute of Biology, Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, 450054 Ufa, RussiaUfa Institute of Biology, Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, 450054 Ufa, RussiaUfa Institute of Biology, Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, 450054 Ufa, RussiaUfa Institute of Biology, Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, 450054 Ufa, RussiaBacteria of the genus <i>Pseudomonas</i> are the most studied microorganisms that biodegrade persistent perfluoroorganic pollutants, and the research of their application for the remediation of environmental sites using biotechnological approaches remains relevant. The aim of this study was to investigate the ability of a known destructor of perfluorooctane sulfonic acid from the genus <i>Pseudomonas</i> to accelerate and enhance the destruction of long-chain perfluorocarboxylic acids (PFCAs), specifically perfluorooctanoic acid and perfluorononanoic acid, in water and soil in association with the strain <i>P</i>. <i>mosselii</i> 5(3), which has previously confirmed genetic potential for the degrading of PFCAs. The complete genome (5.86 million base pairs) of the strain 2,4-D, probably belonging to a new species of <i>Pseudomonas</i>, was sequenced, assembled, and analyzed. The genomes of both strains contain genes involved in the defluorination of fluorinated compounds, including haloacetate dehalogenase H-1 (<i>dehH1</i>) and haloalkane dehalogenase (<i>dhaA</i>). The strain 2,4-D also has a multicomponent enzyme system consisting of a dioxygenase component, an electron carrier, and 2-halobenzoate 1,2-dioxygenase (CbdA) with a preference for fluorides. The strain 2,4-D was able to defluorinate PFCAs in an aqueous cultivation system within 7 days, using them as the sole source of carbon and energy and converting them to perfluorheptanoic acid. It assisted strain 5(3) to convert PFCAs to perfluoropentanoic acid, accelerating the process by 24 h. In a model experiment for the bioaugmentation of microorganisms in artificially contaminated soil, the degradation of PFCAs by the association of pseudomonads also occurred faster and deeper than by the individual strains, achieving a degree of biodestruction of 75% over 60 days, with the perfluoropentanoic acid as the main metabolite. These results are of great importance for the development of methods for the biological recultivation of fluorinated organic pollutants for environmental protection and for understanding the fundamental mechanisms of bacterial interactions with these compounds.https://www.mdpi.com/2305-6304/12/12/930perfluorocarboxylic acidsbiodegradationassociation of bacteria<i>Pseudomonas</i>transformationgenome |
| spellingShingle | Sergey Chetverikov Gaisar Hkudaigulov Danil Sharipov Sergey Starikov Probable New Species of Bacteria of the Genus <i>Pseudomonas</i> Accelerates and Enhances the Destruction of Perfluorocarboxylic Acids Toxics perfluorocarboxylic acids biodegradation association of bacteria <i>Pseudomonas</i> transformation genome |
| title | Probable New Species of Bacteria of the Genus <i>Pseudomonas</i> Accelerates and Enhances the Destruction of Perfluorocarboxylic Acids |
| title_full | Probable New Species of Bacteria of the Genus <i>Pseudomonas</i> Accelerates and Enhances the Destruction of Perfluorocarboxylic Acids |
| title_fullStr | Probable New Species of Bacteria of the Genus <i>Pseudomonas</i> Accelerates and Enhances the Destruction of Perfluorocarboxylic Acids |
| title_full_unstemmed | Probable New Species of Bacteria of the Genus <i>Pseudomonas</i> Accelerates and Enhances the Destruction of Perfluorocarboxylic Acids |
| title_short | Probable New Species of Bacteria of the Genus <i>Pseudomonas</i> Accelerates and Enhances the Destruction of Perfluorocarboxylic Acids |
| title_sort | probable new species of bacteria of the genus i pseudomonas i accelerates and enhances the destruction of perfluorocarboxylic acids |
| topic | perfluorocarboxylic acids biodegradation association of bacteria <i>Pseudomonas</i> transformation genome |
| url | https://www.mdpi.com/2305-6304/12/12/930 |
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