Investigation of the physiological and molecular regulatory mechanism of soluble sugar metabolism in Lavandula angustifolia Mill. under cold stress
Lavender (Lavandula angustifolia Mill.) is a valuable aromatic plant with significant commercial importance. However, cold stress–one of the primary abiotic factors impacting sugar metabolism–adversely affects its agricultural productivity in Northeast China. To investigate the mechanisms underlying...
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| Language: | English |
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1537516/full |
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| author | Yuchen Liang Yuchen Liang Yuchen Liang Yuchen Liang Yinan Liu Yinan Liu Yu Wang Yu Wang Ruijiao Yang Ruijiao Yang Zening Yuan Zening Yuan Hui Li Hui Li |
| author_facet | Yuchen Liang Yuchen Liang Yuchen Liang Yuchen Liang Yinan Liu Yinan Liu Yu Wang Yu Wang Ruijiao Yang Ruijiao Yang Zening Yuan Zening Yuan Hui Li Hui Li |
| author_sort | Yuchen Liang |
| collection | DOAJ |
| description | Lavender (Lavandula angustifolia Mill.) is a valuable aromatic plant with significant commercial importance. However, cold stress–one of the primary abiotic factors impacting sugar metabolism–adversely affects its agricultural productivity in Northeast China. To investigate the mechanisms underlying cold tolerance in L. angustifolia and support economic development, we measured the sugar content and performed transcriptome analysis at temperatures of 30°C (control), 20°C, 10°C, and 0°C. The results revealed that when the temperature dropped from 30°C to 0°C, the amylase activities and the content of maltose and glucose increased, while the starch content decreased. During the process, the up-regulation of LaAMY and LaBAM1/3 suggests an adaptive response in L. angustifolia to cold stress by promoting the breakdown of starch. Meanwhile, the up-regulation of sugar metabolism genes LaRHM1, LaMUR4, LaUGD4, alongside the downregulation of photosynthesis-related genes LaPSAD1, LaPSAN, LaPSBQ2, LaLHCB4.2, and LaPSB27-1 illustrate a strong connection to soluble sugar metabolism. These key genes exhibit significant correlations with starch content and amylase activities, specifically in the decomposition of starch into soluble sugars. The results indicate the decomposition of starch into soluble sugars plays a crucial role in osmotic regulation, facilitating subsequent sugar metabolism in L. angustifolia under cold stress. The correlation between gene expression and physiological indicators suggests that genes can potentially mitigate light-induced damage while promoting cellular homeostasis. Molecular docking analyses between the proteins PSAN and RHM1, MUR4 and UGD4, as well as between LHCB4.2 and RHM1, MUR4, and UGD4 predict that these protein interactions involved in sugar metabolism and photosynthesis contribute to enhancing cold resistance in L. angustifolia. |
| format | Article |
| id | doaj-art-b506b32b0d0440baa6306e00bf8d45cc |
| institution | DOAJ |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-b506b32b0d0440baa6306e00bf8d45cc2025-08-20T03:17:39ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-07-011610.3389/fpls.2025.15375161537516Investigation of the physiological and molecular regulatory mechanism of soluble sugar metabolism in Lavandula angustifolia Mill. under cold stressYuchen Liang0Yuchen Liang1Yuchen Liang2Yuchen Liang3Yinan Liu4Yinan Liu5Yu Wang6Yu Wang7Ruijiao Yang8Ruijiao Yang9Zening Yuan10Zening Yuan11Hui Li12Hui Li13Key Laboratory of Aquatic Biodiversity Research in HeiLongjiang Province, Harbin Normal University, Harbin, ChinaHeilongjiang Provincial Key Laboratory of Plant Biology in Ordinary Colleges and Universities, Harbin Normal University, Harbin, ChinaInstitute of Botany, Chinese Academy of Sciences, Beijing, ChinaBotanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Aquatic Biodiversity Research in HeiLongjiang Province, Harbin Normal University, Harbin, ChinaHeilongjiang Provincial Key Laboratory of Plant Biology in Ordinary Colleges and Universities, Harbin Normal University, Harbin, ChinaKey Laboratory of Aquatic Biodiversity Research in HeiLongjiang Province, Harbin Normal University, Harbin, ChinaHeilongjiang Provincial Key Laboratory of Plant Biology in Ordinary Colleges and Universities, Harbin Normal University, Harbin, ChinaKey Laboratory of Aquatic Biodiversity Research in HeiLongjiang Province, Harbin Normal University, Harbin, ChinaHeilongjiang Provincial Key Laboratory of Plant Biology in Ordinary Colleges and Universities, Harbin Normal University, Harbin, ChinaKey Laboratory of Aquatic Biodiversity Research in HeiLongjiang Province, Harbin Normal University, Harbin, ChinaHeilongjiang Provincial Key Laboratory of Plant Biology in Ordinary Colleges and Universities, Harbin Normal University, Harbin, ChinaInstitute of Botany, Chinese Academy of Sciences, Beijing, ChinaBotanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing, ChinaLavender (Lavandula angustifolia Mill.) is a valuable aromatic plant with significant commercial importance. However, cold stress–one of the primary abiotic factors impacting sugar metabolism–adversely affects its agricultural productivity in Northeast China. To investigate the mechanisms underlying cold tolerance in L. angustifolia and support economic development, we measured the sugar content and performed transcriptome analysis at temperatures of 30°C (control), 20°C, 10°C, and 0°C. The results revealed that when the temperature dropped from 30°C to 0°C, the amylase activities and the content of maltose and glucose increased, while the starch content decreased. During the process, the up-regulation of LaAMY and LaBAM1/3 suggests an adaptive response in L. angustifolia to cold stress by promoting the breakdown of starch. Meanwhile, the up-regulation of sugar metabolism genes LaRHM1, LaMUR4, LaUGD4, alongside the downregulation of photosynthesis-related genes LaPSAD1, LaPSAN, LaPSBQ2, LaLHCB4.2, and LaPSB27-1 illustrate a strong connection to soluble sugar metabolism. These key genes exhibit significant correlations with starch content and amylase activities, specifically in the decomposition of starch into soluble sugars. The results indicate the decomposition of starch into soluble sugars plays a crucial role in osmotic regulation, facilitating subsequent sugar metabolism in L. angustifolia under cold stress. The correlation between gene expression and physiological indicators suggests that genes can potentially mitigate light-induced damage while promoting cellular homeostasis. Molecular docking analyses between the proteins PSAN and RHM1, MUR4 and UGD4, as well as between LHCB4.2 and RHM1, MUR4, and UGD4 predict that these protein interactions involved in sugar metabolism and photosynthesis contribute to enhancing cold resistance in L. angustifolia.https://www.frontiersin.org/articles/10.3389/fpls.2025.1537516/fullLavandula angustifolia Mill.cold stresssugar metabolismkey genesmolecular docking |
| spellingShingle | Yuchen Liang Yuchen Liang Yuchen Liang Yuchen Liang Yinan Liu Yinan Liu Yu Wang Yu Wang Ruijiao Yang Ruijiao Yang Zening Yuan Zening Yuan Hui Li Hui Li Investigation of the physiological and molecular regulatory mechanism of soluble sugar metabolism in Lavandula angustifolia Mill. under cold stress Frontiers in Plant Science Lavandula angustifolia Mill. cold stress sugar metabolism key genes molecular docking |
| title | Investigation of the physiological and molecular regulatory mechanism of soluble sugar metabolism in Lavandula angustifolia Mill. under cold stress |
| title_full | Investigation of the physiological and molecular regulatory mechanism of soluble sugar metabolism in Lavandula angustifolia Mill. under cold stress |
| title_fullStr | Investigation of the physiological and molecular regulatory mechanism of soluble sugar metabolism in Lavandula angustifolia Mill. under cold stress |
| title_full_unstemmed | Investigation of the physiological and molecular regulatory mechanism of soluble sugar metabolism in Lavandula angustifolia Mill. under cold stress |
| title_short | Investigation of the physiological and molecular regulatory mechanism of soluble sugar metabolism in Lavandula angustifolia Mill. under cold stress |
| title_sort | investigation of the physiological and molecular regulatory mechanism of soluble sugar metabolism in lavandula angustifolia mill under cold stress |
| topic | Lavandula angustifolia Mill. cold stress sugar metabolism key genes molecular docking |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1537516/full |
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