A Bacteria Acclimation Technology Based on Nitrogen Source Regulation and Its Application in the Reinforcement of Island and Reef Slopes
Microbially Induced Calcium Carbonate Precipitation (MICP) technology has garnered significant attention in geotechnical engineering and environmental remediation due to its environmentally friendly and cost-effective advantages. However, the current MICP technology faces challenges in practical eng...
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MDPI AG
2025-04-01
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| author | Xin Chen Ziyu Wang Liang Cao Peng Cao Shuyue Liu Yu Xie Yingqi Xie |
| author_facet | Xin Chen Ziyu Wang Liang Cao Peng Cao Shuyue Liu Yu Xie Yingqi Xie |
| author_sort | Xin Chen |
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| description | Microbially Induced Calcium Carbonate Precipitation (MICP) technology has garnered significant attention in geotechnical engineering and environmental remediation due to its environmentally friendly and cost-effective advantages. However, the current MICP technology faces challenges in practical engineering applications, particularly the prolonged cementation time, which makes it difficult to meet the requirements for coastal slope reinforcement. Therefore, this study designed novel cultivation conditions for Sporosarcina pasteurii by regulating external nitrogen source concentration and evaluated its environmental adaptability by measuring OD600, urease activity, and bacterial length. By monitoring the changes in Ca<sup>2+</sup> concentration, pH, and precipitation rate over time during the mineralization process, rapid cementation under MICP conditions was achieved. The engineering applicability of this approach in slope reinforcement was comprehensively assessed through simulated on-site scouring and penetration tests. The reinforcement mechanism and the microstructure of the cementation under novel cultivation conditions were analyzed using scanning electron microscopy (SEM), energy dispersive spectrometry (EDS), and X-ray diffraction (XRD). The results indicated that the activity of Sporosarcina pasteurii in the modified NH<sub>4</sub>-YE medium significantly improved in freshwater environments, and the MICP mineralization reaction was rapid, completing within 4 h. The primary crystal form of the generated precipitate was rhombohedral calcite, which formed a tightly bonded microstructure with calcareous sand, achieving a maximum reinforcement strength of 13.61 MPa. The penetration strength increased by at least 20%, and the precipitation rate improved by at least 2-fold. The scouring morphology remained essentially unchanged within 6 h. The findings of this study provide foundational and theoretical data for the application of MICP reinforcement technology to coastal calcareous sand models. |
| format | Article |
| id | doaj-art-b4fefd798079402588304d7f574e37c2 |
| institution | DOAJ |
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| language | English |
| publishDate | 2025-04-01 |
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| series | Journal of Marine Science and Engineering |
| spelling | doaj-art-b4fefd798079402588304d7f574e37c22025-08-20T03:14:36ZengMDPI AGJournal of Marine Science and Engineering2077-13122025-04-0113584810.3390/jmse13050848A Bacteria Acclimation Technology Based on Nitrogen Source Regulation and Its Application in the Reinforcement of Island and Reef SlopesXin Chen0Ziyu Wang1Liang Cao2Peng Cao3Shuyue Liu4Yu Xie5Yingqi Xie6Yazhou Bay Innovation Institute, College of Marine Science and Technology, Hainan Tropical Ocean University, Sanya 572022, ChinaYazhou Bay Innovation Institute, College of Marine Science and Technology, Hainan Tropical Ocean University, Sanya 572022, ChinaCollege of Architecture and Civil Engineering, Beijing University of Technology, Beijing 100124, ChinaCollege of Architecture and Civil Engineering, Beijing University of Technology, Beijing 100124, ChinaCollege of Architecture and Civil Engineering, Beijing University of Technology, Beijing 100124, ChinaYazhou Bay Innovation Institute, College of Marine Science and Technology, Hainan Tropical Ocean University, Sanya 572022, ChinaYazhou Bay Innovation Institute, College of Marine Science and Technology, Hainan Tropical Ocean University, Sanya 572022, ChinaMicrobially Induced Calcium Carbonate Precipitation (MICP) technology has garnered significant attention in geotechnical engineering and environmental remediation due to its environmentally friendly and cost-effective advantages. However, the current MICP technology faces challenges in practical engineering applications, particularly the prolonged cementation time, which makes it difficult to meet the requirements for coastal slope reinforcement. Therefore, this study designed novel cultivation conditions for Sporosarcina pasteurii by regulating external nitrogen source concentration and evaluated its environmental adaptability by measuring OD600, urease activity, and bacterial length. By monitoring the changes in Ca<sup>2+</sup> concentration, pH, and precipitation rate over time during the mineralization process, rapid cementation under MICP conditions was achieved. The engineering applicability of this approach in slope reinforcement was comprehensively assessed through simulated on-site scouring and penetration tests. The reinforcement mechanism and the microstructure of the cementation under novel cultivation conditions were analyzed using scanning electron microscopy (SEM), energy dispersive spectrometry (EDS), and X-ray diffraction (XRD). The results indicated that the activity of Sporosarcina pasteurii in the modified NH<sub>4</sub>-YE medium significantly improved in freshwater environments, and the MICP mineralization reaction was rapid, completing within 4 h. The primary crystal form of the generated precipitate was rhombohedral calcite, which formed a tightly bonded microstructure with calcareous sand, achieving a maximum reinforcement strength of 13.61 MPa. The penetration strength increased by at least 20%, and the precipitation rate improved by at least 2-fold. The scouring morphology remained essentially unchanged within 6 h. The findings of this study provide foundational and theoretical data for the application of MICP reinforcement technology to coastal calcareous sand models.https://www.mdpi.com/2077-1312/13/5/848<i>Bacillus pasteurii</i>culture mediumMICPcoastal erosioncoastal model |
| spellingShingle | Xin Chen Ziyu Wang Liang Cao Peng Cao Shuyue Liu Yu Xie Yingqi Xie A Bacteria Acclimation Technology Based on Nitrogen Source Regulation and Its Application in the Reinforcement of Island and Reef Slopes Journal of Marine Science and Engineering <i>Bacillus pasteurii</i> culture medium MICP coastal erosion coastal model |
| title | A Bacteria Acclimation Technology Based on Nitrogen Source Regulation and Its Application in the Reinforcement of Island and Reef Slopes |
| title_full | A Bacteria Acclimation Technology Based on Nitrogen Source Regulation and Its Application in the Reinforcement of Island and Reef Slopes |
| title_fullStr | A Bacteria Acclimation Technology Based on Nitrogen Source Regulation and Its Application in the Reinforcement of Island and Reef Slopes |
| title_full_unstemmed | A Bacteria Acclimation Technology Based on Nitrogen Source Regulation and Its Application in the Reinforcement of Island and Reef Slopes |
| title_short | A Bacteria Acclimation Technology Based on Nitrogen Source Regulation and Its Application in the Reinforcement of Island and Reef Slopes |
| title_sort | bacteria acclimation technology based on nitrogen source regulation and its application in the reinforcement of island and reef slopes |
| topic | <i>Bacillus pasteurii</i> culture medium MICP coastal erosion coastal model |
| url | https://www.mdpi.com/2077-1312/13/5/848 |
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