Algorithm and ninhydrin method allow for measurement of the postprandial appearance of peptides in blood
Abstract The recognition of amine groups by ninhydrin, along with a simple mathematical algorithm, showed that di- and tripeptides derived from dietary protein are the major end products of protein digestion entering the blood postprandially. There are thousands of oligopeptides appearing in the gut...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-06-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-04625-w |
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| author | Stefan G. Pierzynowski Piotr Wychowański Wiesław Szczesny Robert Galloto Kamil Zaworski Dominika Szkopek Jarosław Woliński Janine Donaldson Kateryna Pierzynowska |
| author_facet | Stefan G. Pierzynowski Piotr Wychowański Wiesław Szczesny Robert Galloto Kamil Zaworski Dominika Szkopek Jarosław Woliński Janine Donaldson Kateryna Pierzynowska |
| author_sort | Stefan G. Pierzynowski |
| collection | DOAJ |
| description | Abstract The recognition of amine groups by ninhydrin, along with a simple mathematical algorithm, showed that di- and tripeptides derived from dietary protein are the major end products of protein digestion entering the blood postprandially. There are thousands of oligopeptides appearing in the gut during protein digestion. However, the presented study on a pig model clearly shows that peptides longer than tri-amino acid peptides do not appear postprandially in the blood in nutritional amounts. We hypothesize that the measurement of postprandial free amino acids, di- and tripeptides and proportions between these components could be a useful, desirable tool both in laboratory and clinical practice, which could be used to determine the metabolic importance of protein digestion end products in health and disease. |
| format | Article |
| id | doaj-art-b4ebf5a757ef4f1d8e2c7400233f263c |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-b4ebf5a757ef4f1d8e2c7400233f263c2025-08-20T03:26:44ZengNature PortfolioScientific Reports2045-23222025-06-0115111210.1038/s41598-025-04625-wAlgorithm and ninhydrin method allow for measurement of the postprandial appearance of peptides in bloodStefan G. Pierzynowski0Piotr Wychowański1Wiesław Szczesny2Robert Galloto3Kamil Zaworski4Dominika Szkopek5Jarosław Woliński6Janine Donaldson7Kateryna Pierzynowska8Department of Biology, Lund UniversityDepartment of Head and Neck and Sensory Organs, Division of Oral Surgery and Implantology, Institute of Clinical Dentistry, Gemelli Foundation for the University Policlinic, Catholic University of the “Sacred Heart”Anara ABAnagram Therapeutics, IncDepartment of Animal Physiology, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of SciencesDepartment of Animal Physiology, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of SciencesDepartment of Animal Physiology, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of SciencesAnara ABDepartment of Biology, Lund UniversityAbstract The recognition of amine groups by ninhydrin, along with a simple mathematical algorithm, showed that di- and tripeptides derived from dietary protein are the major end products of protein digestion entering the blood postprandially. There are thousands of oligopeptides appearing in the gut during protein digestion. However, the presented study on a pig model clearly shows that peptides longer than tri-amino acid peptides do not appear postprandially in the blood in nutritional amounts. We hypothesize that the measurement of postprandial free amino acids, di- and tripeptides and proportions between these components could be a useful, desirable tool both in laboratory and clinical practice, which could be used to determine the metabolic importance of protein digestion end products in health and disease.https://doi.org/10.1038/s41598-025-04625-wRevisited ninhydrin methodAlgorithmProtein digestion end productsDipeptidesTripeptidesMixed meal tolerance test |
| spellingShingle | Stefan G. Pierzynowski Piotr Wychowański Wiesław Szczesny Robert Galloto Kamil Zaworski Dominika Szkopek Jarosław Woliński Janine Donaldson Kateryna Pierzynowska Algorithm and ninhydrin method allow for measurement of the postprandial appearance of peptides in blood Scientific Reports Revisited ninhydrin method Algorithm Protein digestion end products Dipeptides Tripeptides Mixed meal tolerance test |
| title | Algorithm and ninhydrin method allow for measurement of the postprandial appearance of peptides in blood |
| title_full | Algorithm and ninhydrin method allow for measurement of the postprandial appearance of peptides in blood |
| title_fullStr | Algorithm and ninhydrin method allow for measurement of the postprandial appearance of peptides in blood |
| title_full_unstemmed | Algorithm and ninhydrin method allow for measurement of the postprandial appearance of peptides in blood |
| title_short | Algorithm and ninhydrin method allow for measurement of the postprandial appearance of peptides in blood |
| title_sort | algorithm and ninhydrin method allow for measurement of the postprandial appearance of peptides in blood |
| topic | Revisited ninhydrin method Algorithm Protein digestion end products Dipeptides Tripeptides Mixed meal tolerance test |
| url | https://doi.org/10.1038/s41598-025-04625-w |
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