Comparing Campylobacter jejuni to three other enteric pathogens in OligoMM12 mice reveals pathogen-specific host and microbiota responses
Campylobacter jejuni, non-typhoidal Salmonella spp., Listeria monocytogenes and enteropathogenic/enterohemorrhagic Escherichia coli (EPEC/EHEC) are leading causes of food-borne illness worldwide. Citrobacter rodentium has been used to model EPEC and EHEC infection in mice. The gut microbiome is well...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-12-01
|
| Series: | Gut Microbes |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/19490976.2024.2447832 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832592375579410432 |
|---|---|
| author | Mathias K.-M. Herzog Audrey Peters Nizar Shayya Monica Cazzaniga Kardokh Kaka Bra Trisha Arora Manja Barthel Ersin Gül Luca Maurer Patrick Kiefer Philipp Christen Katharina Endhardt Julia A. Vorholt Gad Frankel Markus M. Heimesaat Stefan Bereswill Cormac G. M. Gahan Marcus J. Claesson Xavier Domingo-Almenara Wolf-Dietrich Hardt |
| author_facet | Mathias K.-M. Herzog Audrey Peters Nizar Shayya Monica Cazzaniga Kardokh Kaka Bra Trisha Arora Manja Barthel Ersin Gül Luca Maurer Patrick Kiefer Philipp Christen Katharina Endhardt Julia A. Vorholt Gad Frankel Markus M. Heimesaat Stefan Bereswill Cormac G. M. Gahan Marcus J. Claesson Xavier Domingo-Almenara Wolf-Dietrich Hardt |
| author_sort | Mathias K.-M. Herzog |
| collection | DOAJ |
| description | Campylobacter jejuni, non-typhoidal Salmonella spp., Listeria monocytogenes and enteropathogenic/enterohemorrhagic Escherichia coli (EPEC/EHEC) are leading causes of food-borne illness worldwide. Citrobacter rodentium has been used to model EPEC and EHEC infection in mice. The gut microbiome is well-known to affect gut colonization and host responses to many food-borne pathogens. Recent progress has established gnotobiotic mice as valuable models to study how microbiota affect the enteric infections by S. Typhimurium, C. rodentium and L. monocytogenes. However, for C. jejuni, we are still lacking a suitable gnotobiotic mouse model. Moreover, the limited comparability of data across laboratories is often negatively affected by variations between different research facilities or murine microbiotas. In this study, we applied the standardized gnotobiotic OligoMM12 microbiota mouse model and compared the infections in the same facility. We provide evidence of robust colonization and significant pathological changes in OligoMM12 mice following infection with these pathogens. Moreover, we offer insights into pathogen-specific host responses and metabolite signatures, highlighting the advantages of a standardized mouse model for direct comparisons of factors influencing the pathogenesis of major food-borne pathogens. Notably, we reveal for the first time that C. jejuni stably colonizes OligoMM12 mice, triggering inflammation. Additionally, our comparative approach successfully identifies pathogen-specific responses, including the detection of genes uniquely associated with C. jejuni infection in humans. These findings underscore the potential of the OligoMM12 model as a versatile tool for advancing our understanding of food-borne pathogen interactions. |
| format | Article |
| id | doaj-art-b4e924c93ba04bef8a60c6b5b7af8d96 |
| institution | Kabale University |
| issn | 1949-0976 1949-0984 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj-art-b4e924c93ba04bef8a60c6b5b7af8d962025-01-21T09:27:00ZengTaylor & Francis GroupGut Microbes1949-09761949-09842025-12-0117110.1080/19490976.2024.2447832Comparing Campylobacter jejuni to three other enteric pathogens in OligoMM12 mice reveals pathogen-specific host and microbiota responsesMathias K.-M. Herzog0Audrey Peters1Nizar Shayya2Monica Cazzaniga3Kardokh Kaka Bra4Trisha Arora5Manja Barthel6Ersin Gül7Luca Maurer8Patrick Kiefer9Philipp Christen10Katharina Endhardt11Julia A. Vorholt12Gad Frankel13Markus M. Heimesaat14Stefan Bereswill15Cormac G. M. Gahan16Marcus J. Claesson17Xavier Domingo-Almenara18Wolf-Dietrich Hardt19Institute of Microbiology, Department of Biology, ETH Zurich, Zurich, SwitzerlandDepartment of Life Sciences, MRC Centre for Bacterial Resistance Biology, Imperial College London, London, UKGastrointestinal Microbiology Research Group, Institute of Microbiology, Infectious Diseases and Immunology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, GermanyAPC Microbiome Ireland, University College Cork, Cork, IrelandAPC Microbiome Ireland, University College Cork, Cork, IrelandOmic Sciences Unit, EURECAT – Technology Centre of Catalonia, Reus, SpainInstitute of Microbiology, Department of Biology, ETH Zurich, Zurich, SwitzerlandInstitute of Microbiology, Department of Biology, ETH Zurich, Zurich, SwitzerlandInstitute of Microbiology, Department of Biology, ETH Zurich, Zurich, SwitzerlandInstitute of Microbiology, Department of Biology, ETH Zurich, Zurich, SwitzerlandInstitute of Microbiology, Department of Biology, ETH Zurich, Zurich, SwitzerlandDepartment of Pathology and Molecular Pathology, University Hospital Zurich, Zurich, SwitzerlandInstitute of Microbiology, Department of Biology, ETH Zurich, Zurich, SwitzerlandDepartment of Life Sciences, MRC Centre for Bacterial Resistance Biology, Imperial College London, London, UKGastrointestinal Microbiology Research Group, Institute of Microbiology, Infectious Diseases and Immunology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, GermanyGastrointestinal Microbiology Research Group, Institute of Microbiology, Infectious Diseases and Immunology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, GermanyAPC Microbiome Ireland, University College Cork, Cork, IrelandAPC Microbiome Ireland, University College Cork, Cork, IrelandOmic Sciences Unit, EURECAT – Technology Centre of Catalonia, Reus, SpainInstitute of Microbiology, Department of Biology, ETH Zurich, Zurich, SwitzerlandCampylobacter jejuni, non-typhoidal Salmonella spp., Listeria monocytogenes and enteropathogenic/enterohemorrhagic Escherichia coli (EPEC/EHEC) are leading causes of food-borne illness worldwide. Citrobacter rodentium has been used to model EPEC and EHEC infection in mice. The gut microbiome is well-known to affect gut colonization and host responses to many food-borne pathogens. Recent progress has established gnotobiotic mice as valuable models to study how microbiota affect the enteric infections by S. Typhimurium, C. rodentium and L. monocytogenes. However, for C. jejuni, we are still lacking a suitable gnotobiotic mouse model. Moreover, the limited comparability of data across laboratories is often negatively affected by variations between different research facilities or murine microbiotas. In this study, we applied the standardized gnotobiotic OligoMM12 microbiota mouse model and compared the infections in the same facility. We provide evidence of robust colonization and significant pathological changes in OligoMM12 mice following infection with these pathogens. Moreover, we offer insights into pathogen-specific host responses and metabolite signatures, highlighting the advantages of a standardized mouse model for direct comparisons of factors influencing the pathogenesis of major food-borne pathogens. Notably, we reveal for the first time that C. jejuni stably colonizes OligoMM12 mice, triggering inflammation. Additionally, our comparative approach successfully identifies pathogen-specific responses, including the detection of genes uniquely associated with C. jejuni infection in humans. These findings underscore the potential of the OligoMM12 model as a versatile tool for advancing our understanding of food-borne pathogen interactions.https://www.tandfonline.com/doi/10.1080/19490976.2024.2447832Infectious diseasesmouse modelmicrobiotaCampylobacter jejuniListeria monocytogenesCitrobacter rodentium |
| spellingShingle | Mathias K.-M. Herzog Audrey Peters Nizar Shayya Monica Cazzaniga Kardokh Kaka Bra Trisha Arora Manja Barthel Ersin Gül Luca Maurer Patrick Kiefer Philipp Christen Katharina Endhardt Julia A. Vorholt Gad Frankel Markus M. Heimesaat Stefan Bereswill Cormac G. M. Gahan Marcus J. Claesson Xavier Domingo-Almenara Wolf-Dietrich Hardt Comparing Campylobacter jejuni to three other enteric pathogens in OligoMM12 mice reveals pathogen-specific host and microbiota responses Gut Microbes Infectious diseases mouse model microbiota Campylobacter jejuni Listeria monocytogenes Citrobacter rodentium |
| title | Comparing Campylobacter jejuni to three other enteric pathogens in OligoMM12 mice reveals pathogen-specific host and microbiota responses |
| title_full | Comparing Campylobacter jejuni to three other enteric pathogens in OligoMM12 mice reveals pathogen-specific host and microbiota responses |
| title_fullStr | Comparing Campylobacter jejuni to three other enteric pathogens in OligoMM12 mice reveals pathogen-specific host and microbiota responses |
| title_full_unstemmed | Comparing Campylobacter jejuni to three other enteric pathogens in OligoMM12 mice reveals pathogen-specific host and microbiota responses |
| title_short | Comparing Campylobacter jejuni to three other enteric pathogens in OligoMM12 mice reveals pathogen-specific host and microbiota responses |
| title_sort | comparing campylobacter jejuni to three other enteric pathogens in oligomm12 mice reveals pathogen specific host and microbiota responses |
| topic | Infectious diseases mouse model microbiota Campylobacter jejuni Listeria monocytogenes Citrobacter rodentium |
| url | https://www.tandfonline.com/doi/10.1080/19490976.2024.2447832 |
| work_keys_str_mv | AT mathiaskmherzog comparingcampylobacterjejunitothreeotherentericpathogensinoligomm12micerevealspathogenspecifichostandmicrobiotaresponses AT audreypeters comparingcampylobacterjejunitothreeotherentericpathogensinoligomm12micerevealspathogenspecifichostandmicrobiotaresponses AT nizarshayya comparingcampylobacterjejunitothreeotherentericpathogensinoligomm12micerevealspathogenspecifichostandmicrobiotaresponses AT monicacazzaniga comparingcampylobacterjejunitothreeotherentericpathogensinoligomm12micerevealspathogenspecifichostandmicrobiotaresponses AT kardokhkakabra comparingcampylobacterjejunitothreeotherentericpathogensinoligomm12micerevealspathogenspecifichostandmicrobiotaresponses AT trishaarora comparingcampylobacterjejunitothreeotherentericpathogensinoligomm12micerevealspathogenspecifichostandmicrobiotaresponses AT manjabarthel comparingcampylobacterjejunitothreeotherentericpathogensinoligomm12micerevealspathogenspecifichostandmicrobiotaresponses AT ersingul comparingcampylobacterjejunitothreeotherentericpathogensinoligomm12micerevealspathogenspecifichostandmicrobiotaresponses AT lucamaurer comparingcampylobacterjejunitothreeotherentericpathogensinoligomm12micerevealspathogenspecifichostandmicrobiotaresponses AT patrickkiefer comparingcampylobacterjejunitothreeotherentericpathogensinoligomm12micerevealspathogenspecifichostandmicrobiotaresponses AT philippchristen comparingcampylobacterjejunitothreeotherentericpathogensinoligomm12micerevealspathogenspecifichostandmicrobiotaresponses AT katharinaendhardt comparingcampylobacterjejunitothreeotherentericpathogensinoligomm12micerevealspathogenspecifichostandmicrobiotaresponses AT juliaavorholt comparingcampylobacterjejunitothreeotherentericpathogensinoligomm12micerevealspathogenspecifichostandmicrobiotaresponses AT gadfrankel comparingcampylobacterjejunitothreeotherentericpathogensinoligomm12micerevealspathogenspecifichostandmicrobiotaresponses AT markusmheimesaat comparingcampylobacterjejunitothreeotherentericpathogensinoligomm12micerevealspathogenspecifichostandmicrobiotaresponses AT stefanbereswill comparingcampylobacterjejunitothreeotherentericpathogensinoligomm12micerevealspathogenspecifichostandmicrobiotaresponses AT cormacgmgahan comparingcampylobacterjejunitothreeotherentericpathogensinoligomm12micerevealspathogenspecifichostandmicrobiotaresponses AT marcusjclaesson comparingcampylobacterjejunitothreeotherentericpathogensinoligomm12micerevealspathogenspecifichostandmicrobiotaresponses AT xavierdomingoalmenara comparingcampylobacterjejunitothreeotherentericpathogensinoligomm12micerevealspathogenspecifichostandmicrobiotaresponses AT wolfdietrichhardt comparingcampylobacterjejunitothreeotherentericpathogensinoligomm12micerevealspathogenspecifichostandmicrobiotaresponses |