Melatonin Attenuates Contrast-Induced Nephropathy in Diabetic Rats: The Role of Interleukin-33 and Oxidative Stress
Background. Inflammation and oxidative stress (OxS) contribute to the pathogenesis of diabetic kidney disease (DKD) and contrast-induced nephropathy (CIN). Patients with DKD were found to be more prone to CIN. Interleukin-33 (IL-33) is a proinflammatory cytokine, but its role in DKD and CIN is unkno...
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| Format: | Article |
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Wiley
2016-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/9050828 |
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| author | Didem Onk Oruc Alper Onk Kultigin Turkmen Huseyin Serkan Erol Tulin Akarsu Ayazoglu Osman Nuri Keles Mesut Halici Ergun Topal |
| author_facet | Didem Onk Oruc Alper Onk Kultigin Turkmen Huseyin Serkan Erol Tulin Akarsu Ayazoglu Osman Nuri Keles Mesut Halici Ergun Topal |
| author_sort | Didem Onk |
| collection | DOAJ |
| description | Background. Inflammation and oxidative stress (OxS) contribute to the pathogenesis of diabetic kidney disease (DKD) and contrast-induced nephropathy (CIN). Patients with DKD were found to be more prone to CIN. Interleukin-33 (IL-33) is a proinflammatory cytokine, but its role in DKD and CIN is unknown. Methods. Thirty male Sprague-Dawley rats were enrolled. The first group was comprised of healthy rats (HRs), whereas the other four groups were made up of diabetic rats (DRs), diabetic rats with contrast-induced nephropathy (CIN + DRs), melatonin-treated diabetic rats (MTDRs), and melatonin-treated CIN + DRs (MTCIN + DRs). All groups except the HRs received 50 mg/kg/day streptozotocin (STZ). CIN + DRs were constituted by administrating 1.5 mg/kg of intravenous radiocontrast dye on the 35th day. MTDRs and MTCIN + DRs were given 20 mg/kg/day of intraperitoneal injection of melatonin (MT) from the 28th day for the constitutive seven days. Results. We observed increased IL-33 in the kidney tissue following induction of CIN in DRs. To determine whether MT is effective in preventing CIN, we administered MT in CIN + DRs and demonstrated that kidney tissue levels of OxS markers, inflammatory cytokines, and IL-33 were significantly diminished in MTCIN + DRs compared with other groups without MT treatment (p<0.05). Conclusion. Inhibition of IL-33 with MT provides therapeutic potential in DKD with CIN. |
| format | Article |
| id | doaj-art-b4e0d3d34f8d43b8a70f749dbef9d5d6 |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-b4e0d3d34f8d43b8a70f749dbef9d5d62025-08-20T02:23:31ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/90508289050828Melatonin Attenuates Contrast-Induced Nephropathy in Diabetic Rats: The Role of Interleukin-33 and Oxidative StressDidem Onk0Oruc Alper Onk1Kultigin Turkmen2Huseyin Serkan Erol3Tulin Akarsu Ayazoglu4Osman Nuri Keles5Mesut Halici6Ergun Topal7Department of Anesthesiology and Reanimation, Faculty of Medicine, Erzincan University, Erzincan, TurkeyDepartment of Cardiovascular Surgery, Faculty of Medicine, Erzincan University, Erzincan, TurkeyDivision of Nephrology, Department of Internal Medicine, Meram School of Medicine, Necmettin Erbakan University, Meram Tıp Fakültesi No. 215, Meram, 42090 Konya, TurkeyDepartment of Biochemistry, Faculty of Veterinary, Ataturk University, Erzurum, TurkeyDepartment of Anesthesiology and Reanimation, Medeniyet University, Goztepe Training and Research Hospital, Istanbul, TurkeyDepartment of Histology and Embryology, Faculty of Medicine, Ataturk University, Erzurum, TurkeyDepartment of Biochemistry, Faculty of Veterinary, Ataturk University, Erzurum, TurkeyDepartment of Cardiology, Faculty of Medicine, Erzincan University, Erzincan, TurkeyBackground. Inflammation and oxidative stress (OxS) contribute to the pathogenesis of diabetic kidney disease (DKD) and contrast-induced nephropathy (CIN). Patients with DKD were found to be more prone to CIN. Interleukin-33 (IL-33) is a proinflammatory cytokine, but its role in DKD and CIN is unknown. Methods. Thirty male Sprague-Dawley rats were enrolled. The first group was comprised of healthy rats (HRs), whereas the other four groups were made up of diabetic rats (DRs), diabetic rats with contrast-induced nephropathy (CIN + DRs), melatonin-treated diabetic rats (MTDRs), and melatonin-treated CIN + DRs (MTCIN + DRs). All groups except the HRs received 50 mg/kg/day streptozotocin (STZ). CIN + DRs were constituted by administrating 1.5 mg/kg of intravenous radiocontrast dye on the 35th day. MTDRs and MTCIN + DRs were given 20 mg/kg/day of intraperitoneal injection of melatonin (MT) from the 28th day for the constitutive seven days. Results. We observed increased IL-33 in the kidney tissue following induction of CIN in DRs. To determine whether MT is effective in preventing CIN, we administered MT in CIN + DRs and demonstrated that kidney tissue levels of OxS markers, inflammatory cytokines, and IL-33 were significantly diminished in MTCIN + DRs compared with other groups without MT treatment (p<0.05). Conclusion. Inhibition of IL-33 with MT provides therapeutic potential in DKD with CIN.http://dx.doi.org/10.1155/2016/9050828 |
| spellingShingle | Didem Onk Oruc Alper Onk Kultigin Turkmen Huseyin Serkan Erol Tulin Akarsu Ayazoglu Osman Nuri Keles Mesut Halici Ergun Topal Melatonin Attenuates Contrast-Induced Nephropathy in Diabetic Rats: The Role of Interleukin-33 and Oxidative Stress Mediators of Inflammation |
| title | Melatonin Attenuates Contrast-Induced Nephropathy in Diabetic Rats: The Role of Interleukin-33 and Oxidative Stress |
| title_full | Melatonin Attenuates Contrast-Induced Nephropathy in Diabetic Rats: The Role of Interleukin-33 and Oxidative Stress |
| title_fullStr | Melatonin Attenuates Contrast-Induced Nephropathy in Diabetic Rats: The Role of Interleukin-33 and Oxidative Stress |
| title_full_unstemmed | Melatonin Attenuates Contrast-Induced Nephropathy in Diabetic Rats: The Role of Interleukin-33 and Oxidative Stress |
| title_short | Melatonin Attenuates Contrast-Induced Nephropathy in Diabetic Rats: The Role of Interleukin-33 and Oxidative Stress |
| title_sort | melatonin attenuates contrast induced nephropathy in diabetic rats the role of interleukin 33 and oxidative stress |
| url | http://dx.doi.org/10.1155/2016/9050828 |
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