Differences in the Microbial Composition and Function of the <i>Arundo donax</i> Rhizosphere Under Different Cultivation Conditions
Rhizosphere microorganisms play an important role in the health and development of root systems. Investigating the microbial composition of the rhizosphere is central to understanding the inter-root microbial function of <i>Arundo donax</i> under various cultivation conditions. To comple...
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2024-12-01
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| author | Fan Yang Miaomiao Liu Xin Wang Yuting Hong Qiuju Yao Xiaoke Chang Gongyao Shi Weiwei Chen Baoming Tian Abeer Hegazy |
| author_facet | Fan Yang Miaomiao Liu Xin Wang Yuting Hong Qiuju Yao Xiaoke Chang Gongyao Shi Weiwei Chen Baoming Tian Abeer Hegazy |
| author_sort | Fan Yang |
| collection | DOAJ |
| description | Rhizosphere microorganisms play an important role in the health and development of root systems. Investigating the microbial composition of the rhizosphere is central to understanding the inter-root microbial function of <i>Arundo donax</i> under various cultivation conditions. To complement the metagenomic study of the <i>Arundo donax</i> rhizosphere, here, an amplicon-based metagenomic survey of bacteria and fungi was selected as a practical approach to analyzing the abundance, diversity index, and community structure of rhizosphere bacteria and fungi, as well as to study the effects of different cultivation methods on rhizosphere microbial diversity. Next-generation sequencing and QIIME2 analysis were used. The results indicated that microbial community richness, diversity, and evenness of the hydroponic samples were lower than those of soil samples when examining the α diversity indices of bacteria and fungi using Chao1, ACE, and Shannon metrics. In particular, the relative abundances of <i>Proteobacteria</i>, <i>Rhizobiales</i>, and <i>Incertae sedis</i> in hydroponic materials were higher, while <i>Basidiomycota</i>, <i>Ascomycota</i>, and <i>Actinobacteriota</i> dominated the flora in soil materials when comparing the numbers of OTUs and the ACE community richness estimator. Furthermore, the rhizosphere of hydroponic <i>A. donax</i> contained a higher abundance of nitrogen-fixing bacteria and photosynthetic bacteria, which contribute to root formation. Additionally, there was a significant presence of <i>Basidiomycota</i>, <i>Ascomycota</i>, and <i>Actinobacteriota</i> in soil <i>A. donax</i>, which can form hyphae. This reveals that the microbial community composition of the <i>A. donax</i> rhizosphere is significantly different under various cultivation conditions, suggesting that employing two distinct culturing techniques for <i>Arundo donax</i> may alter the microbiome. Furthermore, it provides technical support for the synergistic interaction between <i>Arundo donax</i> and rhizosphere microorganisms so as to better use the relationship between <i>Arundo donax</i> and basic microorganisms to solve the problems of <i>Arundo donax</i> growth and ecological restoration. |
| format | Article |
| id | doaj-art-b4d3592eadb44237b8d70fd5779b2577 |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-12-01 |
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| series | Microorganisms |
| spelling | doaj-art-b4d3592eadb44237b8d70fd5779b25772025-08-20T02:50:41ZengMDPI AGMicroorganisms2076-26072024-12-011212264210.3390/microorganisms12122642Differences in the Microbial Composition and Function of the <i>Arundo donax</i> Rhizosphere Under Different Cultivation ConditionsFan Yang0Miaomiao Liu1Xin Wang2Yuting Hong3Qiuju Yao4Xiaoke Chang5Gongyao Shi6Weiwei Chen7Baoming Tian8Abeer Hegazy9Institute of Vegetables, Henan Academy of Agricultural Sciences, Graduate T&R Base of Zhengzhou University, Zhengzhou 450002, ChinaInstitute of Vegetables, Henan Academy of Agricultural Sciences, Graduate T&R Base of Zhengzhou University, Zhengzhou 450002, ChinaInstitute of Vegetables, Henan Academy of Agricultural Sciences, Graduate T&R Base of Zhengzhou University, Zhengzhou 450002, ChinaInstitute of Vegetables, Henan Academy of Agricultural Sciences, Graduate T&R Base of Zhengzhou University, Zhengzhou 450002, ChinaInstitute of Vegetables, Henan Academy of Agricultural Sciences, Graduate T&R Base of Zhengzhou University, Zhengzhou 450002, ChinaInstitute of Vegetables, Henan Academy of Agricultural Sciences, Graduate T&R Base of Zhengzhou University, Zhengzhou 450002, ChinaAgricultural College, Zhengzhou University, Zhengzhou 450001, ChinaAgricultural College, Zhengzhou University, Zhengzhou 450001, ChinaAgricultural College, Zhengzhou University, Zhengzhou 450001, ChinaNational Water Research Center, Shubra El Kheima 13766, EgyptRhizosphere microorganisms play an important role in the health and development of root systems. Investigating the microbial composition of the rhizosphere is central to understanding the inter-root microbial function of <i>Arundo donax</i> under various cultivation conditions. To complement the metagenomic study of the <i>Arundo donax</i> rhizosphere, here, an amplicon-based metagenomic survey of bacteria and fungi was selected as a practical approach to analyzing the abundance, diversity index, and community structure of rhizosphere bacteria and fungi, as well as to study the effects of different cultivation methods on rhizosphere microbial diversity. Next-generation sequencing and QIIME2 analysis were used. The results indicated that microbial community richness, diversity, and evenness of the hydroponic samples were lower than those of soil samples when examining the α diversity indices of bacteria and fungi using Chao1, ACE, and Shannon metrics. In particular, the relative abundances of <i>Proteobacteria</i>, <i>Rhizobiales</i>, and <i>Incertae sedis</i> in hydroponic materials were higher, while <i>Basidiomycota</i>, <i>Ascomycota</i>, and <i>Actinobacteriota</i> dominated the flora in soil materials when comparing the numbers of OTUs and the ACE community richness estimator. Furthermore, the rhizosphere of hydroponic <i>A. donax</i> contained a higher abundance of nitrogen-fixing bacteria and photosynthetic bacteria, which contribute to root formation. Additionally, there was a significant presence of <i>Basidiomycota</i>, <i>Ascomycota</i>, and <i>Actinobacteriota</i> in soil <i>A. donax</i>, which can form hyphae. This reveals that the microbial community composition of the <i>A. donax</i> rhizosphere is significantly different under various cultivation conditions, suggesting that employing two distinct culturing techniques for <i>Arundo donax</i> may alter the microbiome. Furthermore, it provides technical support for the synergistic interaction between <i>Arundo donax</i> and rhizosphere microorganisms so as to better use the relationship between <i>Arundo donax</i> and basic microorganisms to solve the problems of <i>Arundo donax</i> growth and ecological restoration.https://www.mdpi.com/2076-2607/12/12/2642<i>Arundo donax</i>high-throughput sequencingrhizosphere microbial community |
| spellingShingle | Fan Yang Miaomiao Liu Xin Wang Yuting Hong Qiuju Yao Xiaoke Chang Gongyao Shi Weiwei Chen Baoming Tian Abeer Hegazy Differences in the Microbial Composition and Function of the <i>Arundo donax</i> Rhizosphere Under Different Cultivation Conditions Microorganisms <i>Arundo donax</i> high-throughput sequencing rhizosphere microbial community |
| title | Differences in the Microbial Composition and Function of the <i>Arundo donax</i> Rhizosphere Under Different Cultivation Conditions |
| title_full | Differences in the Microbial Composition and Function of the <i>Arundo donax</i> Rhizosphere Under Different Cultivation Conditions |
| title_fullStr | Differences in the Microbial Composition and Function of the <i>Arundo donax</i> Rhizosphere Under Different Cultivation Conditions |
| title_full_unstemmed | Differences in the Microbial Composition and Function of the <i>Arundo donax</i> Rhizosphere Under Different Cultivation Conditions |
| title_short | Differences in the Microbial Composition and Function of the <i>Arundo donax</i> Rhizosphere Under Different Cultivation Conditions |
| title_sort | differences in the microbial composition and function of the i arundo donax i rhizosphere under different cultivation conditions |
| topic | <i>Arundo donax</i> high-throughput sequencing rhizosphere microbial community |
| url | https://www.mdpi.com/2076-2607/12/12/2642 |
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