Cloning, purification and characterization of human dentine matrix protein 1(DMP1)
Introduction: Human teeth are composed mainly of dentin, formed by the odontoblasts. Dentin matrix protein 1 (DMP1) is one of odontoblast differentiation's most important growth factors. Human DMP1 has yet to be completely identified or studied. This study aimed to clone and characterize human...
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| Format: | Article |
| Language: | English |
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Springer
2023-07-01
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| Series: | Saudi Dental Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1013905223000871 |
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| author | Tarek El-Bialy Dror Aizenbud Hamid Sadeghi Paul Scott Itay Aizenbud |
| author_facet | Tarek El-Bialy Dror Aizenbud Hamid Sadeghi Paul Scott Itay Aizenbud |
| author_sort | Tarek El-Bialy |
| collection | DOAJ |
| description | Introduction: Human teeth are composed mainly of dentin, formed by the odontoblasts. Dentin matrix protein 1 (DMP1) is one of odontoblast differentiation's most important growth factors. Human DMP1 has yet to be completely identified or studied. This study aimed to clone and characterize human DMP1. Materials and methods: The DMP1 gene sequence was prepared and cloned by transfection of human 293 cells. Results: The recombinant DMP1 was purified and characterized. Conclusion: The results suggested its future use in dental tissue regeneration and tissue engineering. |
| format | Article |
| id | doaj-art-b4cf9a2b847e46618dd48cd9872b9dfd |
| institution | OA Journals |
| issn | 1013-9052 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | Springer |
| record_format | Article |
| series | Saudi Dental Journal |
| spelling | doaj-art-b4cf9a2b847e46618dd48cd9872b9dfd2025-08-20T02:23:28ZengSpringerSaudi Dental Journal1013-90522023-07-0135555355810.1016/j.sdentj.2023.05.006Cloning, purification and characterization of human dentine matrix protein 1(DMP1)Tarek El-Bialy0Dror Aizenbud1Hamid Sadeghi2Paul Scott3Itay Aizenbud4Division Orthodontics, School of Dentistry, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB, Canada; Corresponding author.Department of Orthodontics and Craniofacial Anomalies, School of Graduate Dentistry, Rambam Health Care Center and Technion Haifa, IsraelDepartment of Dentistry, University of Alberta, Edmonton, CanadaDepartment of Biochemistry, University of Alberta, Edmonton CanadaInstitute of Dental Sciences, Faculty of Dental Medicine, Hebrew University, Jerusalem; Medical Corps, Israel Defense Forces, Tel-Hashomer, IsraelIntroduction: Human teeth are composed mainly of dentin, formed by the odontoblasts. Dentin matrix protein 1 (DMP1) is one of odontoblast differentiation's most important growth factors. Human DMP1 has yet to be completely identified or studied. This study aimed to clone and characterize human DMP1. Materials and methods: The DMP1 gene sequence was prepared and cloned by transfection of human 293 cells. Results: The recombinant DMP1 was purified and characterized. Conclusion: The results suggested its future use in dental tissue regeneration and tissue engineering.http://www.sciencedirect.com/science/article/pii/S1013905223000871Dentine matrix proteinHumanCloningPurification |
| spellingShingle | Tarek El-Bialy Dror Aizenbud Hamid Sadeghi Paul Scott Itay Aizenbud Cloning, purification and characterization of human dentine matrix protein 1(DMP1) Saudi Dental Journal Dentine matrix protein Human Cloning Purification |
| title | Cloning, purification and characterization of human dentine matrix protein 1(DMP1) |
| title_full | Cloning, purification and characterization of human dentine matrix protein 1(DMP1) |
| title_fullStr | Cloning, purification and characterization of human dentine matrix protein 1(DMP1) |
| title_full_unstemmed | Cloning, purification and characterization of human dentine matrix protein 1(DMP1) |
| title_short | Cloning, purification and characterization of human dentine matrix protein 1(DMP1) |
| title_sort | cloning purification and characterization of human dentine matrix protein 1 dmp1 |
| topic | Dentine matrix protein Human Cloning Purification |
| url | http://www.sciencedirect.com/science/article/pii/S1013905223000871 |
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