Diagnosis of bovine rotavirus: an overview of currently available methods
Bovine rotavirus (BRV) is a significant pathogen that causes diarrhea in calves, profoundly impacting the cattle industry and resulting in substantial economic losses. Currently, the established diagnostic approaches for BRV primarily include etiological methods, such as electron microscopy, virus i...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1550601/full |
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| author | Ying Huang Zhi Li Yong Fu Shu Qin Wang Ming Kang Ru Meng |
| author_facet | Ying Huang Zhi Li Yong Fu Shu Qin Wang Ming Kang Ru Meng |
| author_sort | Ying Huang |
| collection | DOAJ |
| description | Bovine rotavirus (BRV) is a significant pathogen that causes diarrhea in calves, profoundly impacting the cattle industry and resulting in substantial economic losses. Currently, the established diagnostic approaches for BRV primarily include etiological methods, such as electron microscopy, virus isolation, and culture; serological methods, including enzyme-linked immunosorbent assay (ELISA), latex agglutination test (LAT), and immunofluorescence techniques; and molecular biological methods, such as reverse transcription-polymerase chain reaction (RT-PCR), real-time quantitative PCR (qPCR), and loop-mediated isothermal amplification (LAMP), as well as next-generation sequencing (NGS) technology. This review summarizes the current diagnostic methods for BRV, discusses their advantages and disadvantages, and presents future perspectives on BRV diagnosis, aiming to provide valuable references for the effective diagnosis and control of BRV-related diseases. |
| format | Article |
| id | doaj-art-b4bb8de1adca49edb521dbfa8457f402 |
| institution | DOAJ |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-b4bb8de1adca49edb521dbfa8457f4022025-08-20T02:45:53ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-02-011610.3389/fmicb.2025.15506011550601Diagnosis of bovine rotavirus: an overview of currently available methodsYing Huang0Zhi Li1Yong Fu2Shu Qin Wang3Ming Kang4Ru Meng5Department of Veterinary Medicine, Qinghai University, Xining, ChinaQinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Xining, ChinaQinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Xining, ChinaXining Animal Disease Control Center, Xining, ChinaDepartment of Veterinary Medicine, Qinghai University, Xining, ChinaXining Animal Disease Control Center, Xining, ChinaBovine rotavirus (BRV) is a significant pathogen that causes diarrhea in calves, profoundly impacting the cattle industry and resulting in substantial economic losses. Currently, the established diagnostic approaches for BRV primarily include etiological methods, such as electron microscopy, virus isolation, and culture; serological methods, including enzyme-linked immunosorbent assay (ELISA), latex agglutination test (LAT), and immunofluorescence techniques; and molecular biological methods, such as reverse transcription-polymerase chain reaction (RT-PCR), real-time quantitative PCR (qPCR), and loop-mediated isothermal amplification (LAMP), as well as next-generation sequencing (NGS) technology. This review summarizes the current diagnostic methods for BRV, discusses their advantages and disadvantages, and presents future perspectives on BRV diagnosis, aiming to provide valuable references for the effective diagnosis and control of BRV-related diseases.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1550601/fullbovine rotavirusdiagnosisetiological methodsserological methodsmolecular biological methods |
| spellingShingle | Ying Huang Zhi Li Yong Fu Shu Qin Wang Ming Kang Ru Meng Diagnosis of bovine rotavirus: an overview of currently available methods Frontiers in Microbiology bovine rotavirus diagnosis etiological methods serological methods molecular biological methods |
| title | Diagnosis of bovine rotavirus: an overview of currently available methods |
| title_full | Diagnosis of bovine rotavirus: an overview of currently available methods |
| title_fullStr | Diagnosis of bovine rotavirus: an overview of currently available methods |
| title_full_unstemmed | Diagnosis of bovine rotavirus: an overview of currently available methods |
| title_short | Diagnosis of bovine rotavirus: an overview of currently available methods |
| title_sort | diagnosis of bovine rotavirus an overview of currently available methods |
| topic | bovine rotavirus diagnosis etiological methods serological methods molecular biological methods |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1550601/full |
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