Optimizing Wastewater Treatment Using Endophytic Fungi Isolated from Acacia saligna L. Bark

Abstract Endophytic fungi’s application in biological wastewater treatment is a cost, clean, and eco-friendly. This study aimed to assess the efficiency of three Aspergillus species in the mycoremediation of industrial (I) and agricultural (A) wastewater. These species were Aspergillus flavipes, Asp...

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Main Authors: Mamdouh S. Serag, Marwa T. Mohesien, Amany F. Hasballah
Format: Article
Language:English
Published: SpringerOpen 2025-02-01
Series:Beni-Suef University Journal of Basic and Applied Sciences
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Online Access:https://doi.org/10.1186/s43088-025-00605-1
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author Mamdouh S. Serag
Marwa T. Mohesien
Amany F. Hasballah
author_facet Mamdouh S. Serag
Marwa T. Mohesien
Amany F. Hasballah
author_sort Mamdouh S. Serag
collection DOAJ
description Abstract Endophytic fungi’s application in biological wastewater treatment is a cost, clean, and eco-friendly. This study aimed to assess the efficiency of three Aspergillus species in the mycoremediation of industrial (I) and agricultural (A) wastewater. These species were Aspergillus flavipes, Aspergillus niger, and Aspergillus flavus isolated from the bark of a medicinal plant (A0cacia saligna). Aspergillus species were subculture on potato dextrose broth at 28 °C for 7 days on a rotatory shaker at 180 rpm until fungal pellets were formed. Before and after treatment with fungi, all wastewater samples were examined for pH, TDS, salinity, EC, COD, BOD, TP, TN, and turbidity. According to the results, Aspergillus flavipes has a good removal efficiency of total phosphorus, removing 78% of it from sample (I) after 25 days, and turbidity removing 91% after 20 days in (A) sample. Aspergillus niger has a high chemical oxygen demand removal efficiency of 99% and 99.8% in (I and A) samples after the 15th and 10th day, respectively, in addition to total nitrogen removal efficiency of 99% in (A) sample after the 25th day; and turbidity removal efficiency of 99.7% in the same sample. Also, Aspergillus flavus recorded high removal of biological oxygen demand by 76% and 66% in (I and A) samples after the 10th day of each, respectively; total nitrogen by 69% after the 10th day in (I) sample; total phosphorus by 23% after 10th day in (A) sample; electrical conductivity by 10.7% after 10th day in (I) sample; and total dissolved salts by 1.3% after 5th day in (I) sample. The results explained that Aspergillus niger and Aspergillus flavus were the most efficient in the removal of chemical oxygen demand and biological oxygen demand which represented indicators of pollution in wastewater. The obtained results will be useful for optimal management of agricultural and industrial wastewater.
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spelling doaj-art-b4bb4206ef924f0fb99c39359b6b1bba2025-08-20T03:11:09ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85432025-02-0114111110.1186/s43088-025-00605-1Optimizing Wastewater Treatment Using Endophytic Fungi Isolated from Acacia saligna L. BarkMamdouh S. Serag0Marwa T. Mohesien1Amany F. Hasballah2Botany and Microbiology Department, Faculty of Science, Damietta UniversityBotany and Microbiology Department, Faculty of Science, Damietta UniversityEnvironmental Sciences Department, Faculty of Sciences, Damietta UniversityAbstract Endophytic fungi’s application in biological wastewater treatment is a cost, clean, and eco-friendly. This study aimed to assess the efficiency of three Aspergillus species in the mycoremediation of industrial (I) and agricultural (A) wastewater. These species were Aspergillus flavipes, Aspergillus niger, and Aspergillus flavus isolated from the bark of a medicinal plant (A0cacia saligna). Aspergillus species were subculture on potato dextrose broth at 28 °C for 7 days on a rotatory shaker at 180 rpm until fungal pellets were formed. Before and after treatment with fungi, all wastewater samples were examined for pH, TDS, salinity, EC, COD, BOD, TP, TN, and turbidity. According to the results, Aspergillus flavipes has a good removal efficiency of total phosphorus, removing 78% of it from sample (I) after 25 days, and turbidity removing 91% after 20 days in (A) sample. Aspergillus niger has a high chemical oxygen demand removal efficiency of 99% and 99.8% in (I and A) samples after the 15th and 10th day, respectively, in addition to total nitrogen removal efficiency of 99% in (A) sample after the 25th day; and turbidity removal efficiency of 99.7% in the same sample. Also, Aspergillus flavus recorded high removal of biological oxygen demand by 76% and 66% in (I and A) samples after the 10th day of each, respectively; total nitrogen by 69% after the 10th day in (I) sample; total phosphorus by 23% after 10th day in (A) sample; electrical conductivity by 10.7% after 10th day in (I) sample; and total dissolved salts by 1.3% after 5th day in (I) sample. The results explained that Aspergillus niger and Aspergillus flavus were the most efficient in the removal of chemical oxygen demand and biological oxygen demand which represented indicators of pollution in wastewater. The obtained results will be useful for optimal management of agricultural and industrial wastewater.https://doi.org/10.1186/s43088-025-00605-1BiosorptionEndophytic fungiBiological oxygen demandWater qualityWastewaterRemoval efficiency
spellingShingle Mamdouh S. Serag
Marwa T. Mohesien
Amany F. Hasballah
Optimizing Wastewater Treatment Using Endophytic Fungi Isolated from Acacia saligna L. Bark
Beni-Suef University Journal of Basic and Applied Sciences
Biosorption
Endophytic fungi
Biological oxygen demand
Water quality
Wastewater
Removal efficiency
title Optimizing Wastewater Treatment Using Endophytic Fungi Isolated from Acacia saligna L. Bark
title_full Optimizing Wastewater Treatment Using Endophytic Fungi Isolated from Acacia saligna L. Bark
title_fullStr Optimizing Wastewater Treatment Using Endophytic Fungi Isolated from Acacia saligna L. Bark
title_full_unstemmed Optimizing Wastewater Treatment Using Endophytic Fungi Isolated from Acacia saligna L. Bark
title_short Optimizing Wastewater Treatment Using Endophytic Fungi Isolated from Acacia saligna L. Bark
title_sort optimizing wastewater treatment using endophytic fungi isolated from acacia saligna l bark
topic Biosorption
Endophytic fungi
Biological oxygen demand
Water quality
Wastewater
Removal efficiency
url https://doi.org/10.1186/s43088-025-00605-1
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AT amanyfhasballah optimizingwastewatertreatmentusingendophyticfungiisolatedfromacaciasalignalbark