Influence of Cryopreservation on the Acrosome Reaction in Hucul Stallion Spermatozoa
The Hucul horse is a Polish primitive breed with a small population size, which highlights the importance of preserving the genetic resources. The cryopreservation of semen is essential for creating gene banks, but its effect on the acrosome reaction in Hucul stallions has not yet been investigated....
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MDPI AG
2025-06-01
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| Series: | Animals |
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| author | Monika Bugno-Poniewierska Monika Bielecka Natalia Pietras Barbara Kij-Mitka Zenon Podstawski Bogusława Długosz |
| author_facet | Monika Bugno-Poniewierska Monika Bielecka Natalia Pietras Barbara Kij-Mitka Zenon Podstawski Bogusława Długosz |
| author_sort | Monika Bugno-Poniewierska |
| collection | DOAJ |
| description | The Hucul horse is a Polish primitive breed with a small population size, which highlights the importance of preserving the genetic resources. The cryopreservation of semen is essential for creating gene banks, but its effect on the acrosome reaction in Hucul stallions has not yet been investigated. The acrosome reaction is one of the most important physiological events associated with the fertilization process. Therefore, our goal was to determine the level of acrosome reaction in chilled and frozen/thawed Hucul stallion semen using the FluoAcro test and the SCA<sup>®</sup> semen analysis system. We found that semen cryopreservation significantly reduced sperm motility and was associated with an increased percentage of acrosome-reacted spermatozoa. It should be noted, however, that, in this case, there was no negative control, and the results may reflect acrosomal damage rather than the elicited responses. Further validation of the methods with equine sperm and the inclusion of a control are recommended. |
| format | Article |
| id | doaj-art-b4b4b1cf7b2d40bca45ab06cb6b9a839 |
| institution | DOAJ |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-b4b4b1cf7b2d40bca45ab06cb6b9a8392025-08-20T03:16:46ZengMDPI AGAnimals2076-26152025-06-011513191510.3390/ani15131915Influence of Cryopreservation on the Acrosome Reaction in Hucul Stallion SpermatozoaMonika Bugno-Poniewierska0Monika Bielecka1Natalia Pietras2Barbara Kij-Mitka3Zenon Podstawski4Bogusława Długosz5Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Krakow, al. Mickiewicza 24/28, 30-059 Krakow, PolandDepartment of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Krakow, al. Mickiewicza 24/28, 30-059 Krakow, PolandDepartment of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Krakow, al. Mickiewicza 24/28, 30-059 Krakow, PolandDepartment of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Krakow, al. Mickiewicza 24/28, 30-059 Krakow, PolandDepartment of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Krakow, al. Mickiewicza 24/28, 30-059 Krakow, PolandDepartment of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Krakow, al. Mickiewicza 24/28, 30-059 Krakow, PolandThe Hucul horse is a Polish primitive breed with a small population size, which highlights the importance of preserving the genetic resources. The cryopreservation of semen is essential for creating gene banks, but its effect on the acrosome reaction in Hucul stallions has not yet been investigated. The acrosome reaction is one of the most important physiological events associated with the fertilization process. Therefore, our goal was to determine the level of acrosome reaction in chilled and frozen/thawed Hucul stallion semen using the FluoAcro test and the SCA<sup>®</sup> semen analysis system. We found that semen cryopreservation significantly reduced sperm motility and was associated with an increased percentage of acrosome-reacted spermatozoa. It should be noted, however, that, in this case, there was no negative control, and the results may reflect acrosomal damage rather than the elicited responses. Further validation of the methods with equine sperm and the inclusion of a control are recommended.https://www.mdpi.com/2076-2615/15/13/1915stallionspermacrosome reactioncryopreservationHucul horsesCASA system |
| spellingShingle | Monika Bugno-Poniewierska Monika Bielecka Natalia Pietras Barbara Kij-Mitka Zenon Podstawski Bogusława Długosz Influence of Cryopreservation on the Acrosome Reaction in Hucul Stallion Spermatozoa Animals stallion sperm acrosome reaction cryopreservation Hucul horses CASA system |
| title | Influence of Cryopreservation on the Acrosome Reaction in Hucul Stallion Spermatozoa |
| title_full | Influence of Cryopreservation on the Acrosome Reaction in Hucul Stallion Spermatozoa |
| title_fullStr | Influence of Cryopreservation on the Acrosome Reaction in Hucul Stallion Spermatozoa |
| title_full_unstemmed | Influence of Cryopreservation on the Acrosome Reaction in Hucul Stallion Spermatozoa |
| title_short | Influence of Cryopreservation on the Acrosome Reaction in Hucul Stallion Spermatozoa |
| title_sort | influence of cryopreservation on the acrosome reaction in hucul stallion spermatozoa |
| topic | stallion sperm acrosome reaction cryopreservation Hucul horses CASA system |
| url | https://www.mdpi.com/2076-2615/15/13/1915 |
| work_keys_str_mv | AT monikabugnoponiewierska influenceofcryopreservationontheacrosomereactioninhuculstallionspermatozoa AT monikabielecka influenceofcryopreservationontheacrosomereactioninhuculstallionspermatozoa AT nataliapietras influenceofcryopreservationontheacrosomereactioninhuculstallionspermatozoa AT barbarakijmitka influenceofcryopreservationontheacrosomereactioninhuculstallionspermatozoa AT zenonpodstawski influenceofcryopreservationontheacrosomereactioninhuculstallionspermatozoa AT bogusławadługosz influenceofcryopreservationontheacrosomereactioninhuculstallionspermatozoa |