Phenolic Extract from Moringa oleifera Leaves Inhibits Key Enzymes Linked to Erectile Dysfunction and Oxidative Stress in Rats’ Penile Tissues
This study was designed to determine the antioxidant properties and inhibitory effects of extract from Moringa oleifera leaves on angiotensin-I-converting enzyme (ACE) and arginase activities in vitro. The extract was prepared and phenolic (total phenols and flavonoid) contents, radical (nitric oxid...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2015/175950 |
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| author | Ganiyu Oboh Adedayo O. Ademiluyi Ayokunle O. Ademosun Tosin A. Olasehinde Sunday I. Oyeleye Aline A. Boligon Margareth L. Athayde |
| author_facet | Ganiyu Oboh Adedayo O. Ademiluyi Ayokunle O. Ademosun Tosin A. Olasehinde Sunday I. Oyeleye Aline A. Boligon Margareth L. Athayde |
| author_sort | Ganiyu Oboh |
| collection | DOAJ |
| description | This study was designed to determine the antioxidant properties and inhibitory effects of extract from Moringa oleifera leaves on angiotensin-I-converting enzyme (ACE) and arginase activities in vitro. The extract was prepared and phenolic (total phenols and flavonoid) contents, radical (nitric oxide (NO), hydroxyl (OH)) scavenging abilities, and Fe2+-chelating ability were assessed. Characterization of the phenolic constituents was done via high performance liquid chromatography-diode array detection (HPLC-DAD) analysis. Furthermore, the effects of the extract on Fe2+-induced MDA production in rats’ penile tissue homogenate as well as its action on ACE and arginase activities were also determined. The extract scavenged NO∗, OH∗, chelated Fe2+, and inhibited MDA production in a dose-dependent pattern with IC50 values of 1.36, 0.52, and 0.38 mg/mL and 194.23 µg/mL, respectively. Gallic acid, chlorogenic acid, quercetin, and kaempferol were the most abundant phenolic compounds identified in the leaf extract. The extract also inhibited ACE and arginase activities in a dose-dependent pattern and their IC50 values were 303.03 and 159.59 µg/mL, respectively. The phenolic contents, inhibition of ACE, arginase, and Fe2+-induced MDA production, and radical (OH∗, NO∗) scavenging and Fe2+-chelating abilities could be some of the possible mechanisms by which M. oleifera leaves could be used in the treatment and/or management of erectile dysfunction. |
| format | Article |
| id | doaj-art-b4acda19166443f693cdeb57ec8aa447 |
| institution | OA Journals |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-b4acda19166443f693cdeb57ec8aa4472025-08-20T02:23:30ZengWileyBiochemistry Research International2090-22472090-22552015-01-01201510.1155/2015/175950175950Phenolic Extract from Moringa oleifera Leaves Inhibits Key Enzymes Linked to Erectile Dysfunction and Oxidative Stress in Rats’ Penile TissuesGaniyu Oboh0Adedayo O. Ademiluyi1Ayokunle O. Ademosun2Tosin A. Olasehinde3Sunday I. Oyeleye4Aline A. Boligon5Margareth L. Athayde6Functional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of Technology, PMB 704, Akure 340001, NigeriaFunctional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of Technology, PMB 704, Akure 340001, NigeriaFunctional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of Technology, PMB 704, Akure 340001, NigeriaNutrition and Toxicology Division, Food Technology Department, Federal Institute of Industrial Research, Oshodi, PMB 21023, Lagos 10001, NigeriaFunctional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of Technology, PMB 704, Akure 340001, NigeriaPhytochemical Research Laboratory, Department of Industrial Pharmacy, Federal University of Santa Maria, Building 26, Room 1115, 97105-900 Santa Maria, RS, BrazilPhytochemical Research Laboratory, Department of Industrial Pharmacy, Federal University of Santa Maria, Building 26, Room 1115, 97105-900 Santa Maria, RS, BrazilThis study was designed to determine the antioxidant properties and inhibitory effects of extract from Moringa oleifera leaves on angiotensin-I-converting enzyme (ACE) and arginase activities in vitro. The extract was prepared and phenolic (total phenols and flavonoid) contents, radical (nitric oxide (NO), hydroxyl (OH)) scavenging abilities, and Fe2+-chelating ability were assessed. Characterization of the phenolic constituents was done via high performance liquid chromatography-diode array detection (HPLC-DAD) analysis. Furthermore, the effects of the extract on Fe2+-induced MDA production in rats’ penile tissue homogenate as well as its action on ACE and arginase activities were also determined. The extract scavenged NO∗, OH∗, chelated Fe2+, and inhibited MDA production in a dose-dependent pattern with IC50 values of 1.36, 0.52, and 0.38 mg/mL and 194.23 µg/mL, respectively. Gallic acid, chlorogenic acid, quercetin, and kaempferol were the most abundant phenolic compounds identified in the leaf extract. The extract also inhibited ACE and arginase activities in a dose-dependent pattern and their IC50 values were 303.03 and 159.59 µg/mL, respectively. The phenolic contents, inhibition of ACE, arginase, and Fe2+-induced MDA production, and radical (OH∗, NO∗) scavenging and Fe2+-chelating abilities could be some of the possible mechanisms by which M. oleifera leaves could be used in the treatment and/or management of erectile dysfunction.http://dx.doi.org/10.1155/2015/175950 |
| spellingShingle | Ganiyu Oboh Adedayo O. Ademiluyi Ayokunle O. Ademosun Tosin A. Olasehinde Sunday I. Oyeleye Aline A. Boligon Margareth L. Athayde Phenolic Extract from Moringa oleifera Leaves Inhibits Key Enzymes Linked to Erectile Dysfunction and Oxidative Stress in Rats’ Penile Tissues Biochemistry Research International |
| title | Phenolic Extract from Moringa oleifera Leaves Inhibits Key Enzymes Linked to Erectile Dysfunction and Oxidative Stress in Rats’ Penile Tissues |
| title_full | Phenolic Extract from Moringa oleifera Leaves Inhibits Key Enzymes Linked to Erectile Dysfunction and Oxidative Stress in Rats’ Penile Tissues |
| title_fullStr | Phenolic Extract from Moringa oleifera Leaves Inhibits Key Enzymes Linked to Erectile Dysfunction and Oxidative Stress in Rats’ Penile Tissues |
| title_full_unstemmed | Phenolic Extract from Moringa oleifera Leaves Inhibits Key Enzymes Linked to Erectile Dysfunction and Oxidative Stress in Rats’ Penile Tissues |
| title_short | Phenolic Extract from Moringa oleifera Leaves Inhibits Key Enzymes Linked to Erectile Dysfunction and Oxidative Stress in Rats’ Penile Tissues |
| title_sort | phenolic extract from moringa oleifera leaves inhibits key enzymes linked to erectile dysfunction and oxidative stress in rats penile tissues |
| url | http://dx.doi.org/10.1155/2015/175950 |
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