A streamlined POCT solution for rapid infectious disease detection
Abstract Point-of-care testing (POCT) plays a crucial role in infectious disease screening due to its rapid detection and portability. However, current POCT systems face challenges such as lengthy sample preparation time, complex nucleic acid extraction, and limited real-time data transmission, exte...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-04-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-97155-4 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850170846797103104 |
|---|---|
| author | Yaping Xie Yan Yu Shuaiwu Huang Liangcheng Wan Chang Wu Chiwei Yin Jianjun Li Jiangang Ling Lizhong Dai |
| author_facet | Yaping Xie Yan Yu Shuaiwu Huang Liangcheng Wan Chang Wu Chiwei Yin Jianjun Li Jiangang Ling Lizhong Dai |
| author_sort | Yaping Xie |
| collection | DOAJ |
| description | Abstract Point-of-care testing (POCT) plays a crucial role in infectious disease screening due to its rapid detection and portability. However, current POCT systems face challenges such as lengthy sample preparation time, complex nucleic acid extraction, and limited real-time data transmission, extending patient waiting time. This study presents iPonatic, an integrated POCT system with a quantitative dropper for efficient sample addition, a low-cost cartridge utilizing one-step rapid nucleic acid release technology, and real-time data synchronization via a cloud platform. Patients can receive test reports within 30 min. The system was validated for both respiratory and sexually transmitted pathogens, showing consistent $$\:Ct$$ values with standard quantitative PCR. Clinical validation on 1159 samples demonstrated an AUC above 0.98 for all targets, with sensitivity exceeding 95% and specificity reaching 100%. iPonatic is ideal for primary healthcare, epidemic response, and resource-limited settings. It offers an efficient solution to enhance diagnostics and reduce patient waiting time, supporting timely responses to public health needs. |
| format | Article |
| id | doaj-art-b4a68e2073b748eb9328f6b61301dbd0 |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-b4a68e2073b748eb9328f6b61301dbd02025-08-20T02:20:23ZengNature PortfolioScientific Reports2045-23222025-04-0115111010.1038/s41598-025-97155-4A streamlined POCT solution for rapid infectious disease detectionYaping Xie0Yan Yu1Shuaiwu Huang2Liangcheng Wan3Chang Wu4Chiwei Yin5Jianjun Li6Jiangang Ling7Lizhong Dai8Research and Development Department, Sansure Biotech Inc.Research and Development Department, Sansure Biotech Inc.Research and Development Department, Sansure Biotech Inc.Research and Development Department, Sansure Biotech Inc.Research and Development Department, Sansure Biotech Inc.Research and Development Department, Sansure Biotech Inc.Research and Development Department, Sansure Biotech Inc.Research and Development Department, Sansure Biotech Inc.Research and Development Department, Sansure Biotech Inc.Abstract Point-of-care testing (POCT) plays a crucial role in infectious disease screening due to its rapid detection and portability. However, current POCT systems face challenges such as lengthy sample preparation time, complex nucleic acid extraction, and limited real-time data transmission, extending patient waiting time. This study presents iPonatic, an integrated POCT system with a quantitative dropper for efficient sample addition, a low-cost cartridge utilizing one-step rapid nucleic acid release technology, and real-time data synchronization via a cloud platform. Patients can receive test reports within 30 min. The system was validated for both respiratory and sexually transmitted pathogens, showing consistent $$\:Ct$$ values with standard quantitative PCR. Clinical validation on 1159 samples demonstrated an AUC above 0.98 for all targets, with sensitivity exceeding 95% and specificity reaching 100%. iPonatic is ideal for primary healthcare, epidemic response, and resource-limited settings. It offers an efficient solution to enhance diagnostics and reduce patient waiting time, supporting timely responses to public health needs.https://doi.org/10.1038/s41598-025-97155-4Point-of-careQuantitative dropperOne-step nucleic acid release technologyiPonaticRespiratory infectionSexually transmitted infection |
| spellingShingle | Yaping Xie Yan Yu Shuaiwu Huang Liangcheng Wan Chang Wu Chiwei Yin Jianjun Li Jiangang Ling Lizhong Dai A streamlined POCT solution for rapid infectious disease detection Scientific Reports Point-of-care Quantitative dropper One-step nucleic acid release technology iPonatic Respiratory infection Sexually transmitted infection |
| title | A streamlined POCT solution for rapid infectious disease detection |
| title_full | A streamlined POCT solution for rapid infectious disease detection |
| title_fullStr | A streamlined POCT solution for rapid infectious disease detection |
| title_full_unstemmed | A streamlined POCT solution for rapid infectious disease detection |
| title_short | A streamlined POCT solution for rapid infectious disease detection |
| title_sort | streamlined poct solution for rapid infectious disease detection |
| topic | Point-of-care Quantitative dropper One-step nucleic acid release technology iPonatic Respiratory infection Sexually transmitted infection |
| url | https://doi.org/10.1038/s41598-025-97155-4 |
| work_keys_str_mv | AT yapingxie astreamlinedpoctsolutionforrapidinfectiousdiseasedetection AT yanyu astreamlinedpoctsolutionforrapidinfectiousdiseasedetection AT shuaiwuhuang astreamlinedpoctsolutionforrapidinfectiousdiseasedetection AT liangchengwan astreamlinedpoctsolutionforrapidinfectiousdiseasedetection AT changwu astreamlinedpoctsolutionforrapidinfectiousdiseasedetection AT chiweiyin astreamlinedpoctsolutionforrapidinfectiousdiseasedetection AT jianjunli astreamlinedpoctsolutionforrapidinfectiousdiseasedetection AT jiangangling astreamlinedpoctsolutionforrapidinfectiousdiseasedetection AT lizhongdai astreamlinedpoctsolutionforrapidinfectiousdiseasedetection AT yapingxie streamlinedpoctsolutionforrapidinfectiousdiseasedetection AT yanyu streamlinedpoctsolutionforrapidinfectiousdiseasedetection AT shuaiwuhuang streamlinedpoctsolutionforrapidinfectiousdiseasedetection AT liangchengwan streamlinedpoctsolutionforrapidinfectiousdiseasedetection AT changwu streamlinedpoctsolutionforrapidinfectiousdiseasedetection AT chiweiyin streamlinedpoctsolutionforrapidinfectiousdiseasedetection AT jianjunli streamlinedpoctsolutionforrapidinfectiousdiseasedetection AT jiangangling streamlinedpoctsolutionforrapidinfectiousdiseasedetection AT lizhongdai streamlinedpoctsolutionforrapidinfectiousdiseasedetection |