Research of neuroprotective properties of TrkA-receptor agonist GK-2 on model of oxidative stress in human vascular endothelial cells (HUVEC)

Oxidative stress resulted to decrease in cells viability compared to the control (78 ± 5 % and 100 ± 6 %, respectively, р < 0,05). GK-2 and NGF prevented H2O2-induced HUVEC cells damages (95 ± 3 % for HA-2 and 97 ± 4 % for NGF vs. 78 ± 5 % for H2O2, p < 0.05). In control the number of cells wi...

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Main Authors: T. A. Antipova, S. V. Nikolaev, S. A. Kryzhanovsky, E. S. Pekeldina
Format: Article
Language:Russian
Published: LLC “Publisher OKI” 2019-01-01
Series:Фармакокинетика и Фармакодинамика
Subjects:
Online Access:https://www.pharmacokinetica.ru/jour/article/view/82
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author T. A. Antipova
S. V. Nikolaev
S. A. Kryzhanovsky
E. S. Pekeldina
author_facet T. A. Antipova
S. V. Nikolaev
S. A. Kryzhanovsky
E. S. Pekeldina
author_sort T. A. Antipova
collection DOAJ
description Oxidative stress resulted to decrease in cells viability compared to the control (78 ± 5 % and 100 ± 6 %, respectively, р < 0,05). GK-2 and NGF prevented H2O2-induced HUVEC cells damages (95 ± 3 % for HA-2 and 97 ± 4 % for NGF vs. 78 ± 5 % for H2O2, p < 0.05). In control the number of cells with condensed chromatin was 9 ± 2, after the addition of H2O2 78 ± 9 (p < 0.05 compared with the control). The addition of NGF or GK-2 after H2O2 significantly reduced the number of such cells (44 ± 9 and 41 ± 8, respectively) (p < 0.05 compared to hydrogen peroxide) and prevented the development of morphological changes in nuclear chromatin. Thus GK-2, like NGF, in human endothelial cell culture HUVEC prevented the apoptosis development caused by hydrogen peroxide.
format Article
id doaj-art-b4a3bbbdd58542a9abc034b9a4a241a9
institution Kabale University
issn 2587-7836
2686-8830
language Russian
publishDate 2019-01-01
publisher LLC “Publisher OKI”
record_format Article
series Фармакокинетика и Фармакодинамика
spelling doaj-art-b4a3bbbdd58542a9abc034b9a4a241a92025-08-20T03:42:31ZrusLLC “Publisher OKI”Фармакокинетика и Фармакодинамика2587-78362686-88302019-01-0101182110.24411/2587-7836-2019-1003581Research of neuroprotective properties of TrkA-receptor agonist GK-2 on model of oxidative stress in human vascular endothelial cells (HUVEC)T. A. Antipova0S. V. Nikolaev1S. A. Kryzhanovsky2E. S. Pekeldina3FSBI «Zakusov Institute of Pharmacology»FSBI «Zakusov Institute of Pharmacology»FSBI «Zakusov Institute of Pharmacology»FSBI «Zakusov Institute of Pharmacology»Oxidative stress resulted to decrease in cells viability compared to the control (78 ± 5 % and 100 ± 6 %, respectively, р < 0,05). GK-2 and NGF prevented H2O2-induced HUVEC cells damages (95 ± 3 % for HA-2 and 97 ± 4 % for NGF vs. 78 ± 5 % for H2O2, p < 0.05). In control the number of cells with condensed chromatin was 9 ± 2, after the addition of H2O2 78 ± 9 (p < 0.05 compared with the control). The addition of NGF or GK-2 after H2O2 significantly reduced the number of such cells (44 ± 9 and 41 ± 8, respectively) (p < 0.05 compared to hydrogen peroxide) and prevented the development of morphological changes in nuclear chromatin. Thus GK-2, like NGF, in human endothelial cell culture HUVEC prevented the apoptosis development caused by hydrogen peroxide.https://www.pharmacokinetica.ru/jour/article/view/82ngfдимерный дипептидный миметик гк-2huvec клетки эндотелия человекаокислительный стрессапоптозngfdimeric dipeptide mimetic gk-2huvecoxidative stressapoptosis
spellingShingle T. A. Antipova
S. V. Nikolaev
S. A. Kryzhanovsky
E. S. Pekeldina
Research of neuroprotective properties of TrkA-receptor agonist GK-2 on model of oxidative stress in human vascular endothelial cells (HUVEC)
Фармакокинетика и Фармакодинамика
ngf
димерный дипептидный миметик гк-2
huvec клетки эндотелия человека
окислительный стресс
апоптоз
ngf
dimeric dipeptide mimetic gk-2
huvec
oxidative stress
apoptosis
title Research of neuroprotective properties of TrkA-receptor agonist GK-2 on model of oxidative stress in human vascular endothelial cells (HUVEC)
title_full Research of neuroprotective properties of TrkA-receptor agonist GK-2 on model of oxidative stress in human vascular endothelial cells (HUVEC)
title_fullStr Research of neuroprotective properties of TrkA-receptor agonist GK-2 on model of oxidative stress in human vascular endothelial cells (HUVEC)
title_full_unstemmed Research of neuroprotective properties of TrkA-receptor agonist GK-2 on model of oxidative stress in human vascular endothelial cells (HUVEC)
title_short Research of neuroprotective properties of TrkA-receptor agonist GK-2 on model of oxidative stress in human vascular endothelial cells (HUVEC)
title_sort research of neuroprotective properties of trka receptor agonist gk 2 on model of oxidative stress in human vascular endothelial cells huvec
topic ngf
димерный дипептидный миметик гк-2
huvec клетки эндотелия человека
окислительный стресс
апоптоз
ngf
dimeric dipeptide mimetic gk-2
huvec
oxidative stress
apoptosis
url https://www.pharmacokinetica.ru/jour/article/view/82
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AT svnikolaev researchofneuroprotectivepropertiesoftrkareceptoragonistgk2onmodelofoxidativestressinhumanvascularendothelialcellshuvec
AT sakryzhanovsky researchofneuroprotectivepropertiesoftrkareceptoragonistgk2onmodelofoxidativestressinhumanvascularendothelialcellshuvec
AT espekeldina researchofneuroprotectivepropertiesoftrkareceptoragonistgk2onmodelofoxidativestressinhumanvascularendothelialcellshuvec