Expression study of Wnt/β-catenin signaling pathway associated lncRNAs in schizophrenia
Abstract Schizophrenia is one of the most debilitating mental illnesses affecting any age group. The mechanism and etiology of schizophrenia are extremely complex and multiple signaling pathways recruit genes implicated in the etiology of this disease. While the role of Wnt/β-catenin signaling in th...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-01-01
|
| Series: | Annals of General Psychiatry |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12991-025-00545-1 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832594504145698816 |
|---|---|
| author | Fatemeh Manafzadeh Behzad Baradaran Seyed Gholamreza Noor Azar Kamran Javidi Aghdam Reza Dabbaghipour Asghar Shayannia Soudeh Ghafouri-Fard |
| author_facet | Fatemeh Manafzadeh Behzad Baradaran Seyed Gholamreza Noor Azar Kamran Javidi Aghdam Reza Dabbaghipour Asghar Shayannia Soudeh Ghafouri-Fard |
| author_sort | Fatemeh Manafzadeh |
| collection | DOAJ |
| description | Abstract Schizophrenia is one of the most debilitating mental illnesses affecting any age group. The mechanism and etiology of schizophrenia are extremely complex and multiple signaling pathways recruit genes implicated in the etiology of this disease. While the role of Wnt/β-catenin signaling in this disorder has been verified, the impact of long noncoding RNAs (lncRNAs) associated with this pathway has not been studied in schizophrenia. The objective of this study was to examine the expression levels of Wnt/β-catenin-related lncRNAs, namely CCAT2, SNHG5, PTCSC3, and DANCR, as well as the CTNNB1 gene encoding beta-catenin protein in two groups of schizophrenia patients (drug-naïve and medicated) compared with healthy individuals. This study included 50 medicated patients in the remission phase of the disease, 25 drug-naive patients in the acute phase, and 50 control subjects. There was no significant difference in CTNNB1 gene expression in the medicated patients compared to controls (P value = 0.9754). However, the expression of this gene was significantly decreased in drug-naïve first-episode patients compared with controls (P value < 0.001). In contrast, expression of DANCR, PTCSC3, SNHG5, and CCAT2 genes was significantly higher in medicated (P values < 0.001, < 0.001, = 0.01, < 0.001, respectively) and drug-naive first-episode patients (P value < 0.001) compared to control subjects. ROC curve analysis revealed that DANCR, PTCSC3, SNHG5, and CCAT2 genes had diagnostic power with specificity and sensitivity of 80% and above in separation between study subgroups. In brief, our data demonstrated dysregulation of Wnt/β pathway related genes and lncRNAs in the peripheral blood of patients with schizophrenia and their potential as biomarkers for this disorder. |
| format | Article |
| id | doaj-art-b4a39858c2574a5895c44dfd5e82c92a |
| institution | Kabale University |
| issn | 1744-859X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Annals of General Psychiatry |
| spelling | doaj-art-b4a39858c2574a5895c44dfd5e82c92a2025-01-19T12:34:09ZengBMCAnnals of General Psychiatry1744-859X2025-01-0124111010.1186/s12991-025-00545-1Expression study of Wnt/β-catenin signaling pathway associated lncRNAs in schizophreniaFatemeh Manafzadeh0Behzad Baradaran1Seyed Gholamreza Noor Azar2Kamran Javidi Aghdam3Reza Dabbaghipour4Asghar Shayannia5Soudeh Ghafouri-Fard6Student Research Committee, School of Medicine, Shahroud University of Medical SciencesImmunology Research Center, Tabriz University of Medical SciencesResearch Center of Psychiatry and Behavioral Sciences, Tabriz University of Medical SciencesStudent Research Committee, School of Medicine, Shahroud University of Medical SciencesDepartment of Medical Genetics, School of Medicine, Tehran University of Medical SciencesDepartment of Medical Biotechnology, School of Medicine, Shahroud University of Medical SciencesDepartment of Medical Genetics, Shahid Beheshti University of Medical SciencesAbstract Schizophrenia is one of the most debilitating mental illnesses affecting any age group. The mechanism and etiology of schizophrenia are extremely complex and multiple signaling pathways recruit genes implicated in the etiology of this disease. While the role of Wnt/β-catenin signaling in this disorder has been verified, the impact of long noncoding RNAs (lncRNAs) associated with this pathway has not been studied in schizophrenia. The objective of this study was to examine the expression levels of Wnt/β-catenin-related lncRNAs, namely CCAT2, SNHG5, PTCSC3, and DANCR, as well as the CTNNB1 gene encoding beta-catenin protein in two groups of schizophrenia patients (drug-naïve and medicated) compared with healthy individuals. This study included 50 medicated patients in the remission phase of the disease, 25 drug-naive patients in the acute phase, and 50 control subjects. There was no significant difference in CTNNB1 gene expression in the medicated patients compared to controls (P value = 0.9754). However, the expression of this gene was significantly decreased in drug-naïve first-episode patients compared with controls (P value < 0.001). In contrast, expression of DANCR, PTCSC3, SNHG5, and CCAT2 genes was significantly higher in medicated (P values < 0.001, < 0.001, = 0.01, < 0.001, respectively) and drug-naive first-episode patients (P value < 0.001) compared to control subjects. ROC curve analysis revealed that DANCR, PTCSC3, SNHG5, and CCAT2 genes had diagnostic power with specificity and sensitivity of 80% and above in separation between study subgroups. In brief, our data demonstrated dysregulation of Wnt/β pathway related genes and lncRNAs in the peripheral blood of patients with schizophrenia and their potential as biomarkers for this disorder.https://doi.org/10.1186/s12991-025-00545-1SchizophreniaAntipsychotic drugsWnt/β-catenin pathwayΒ-cateninlncRNA |
| spellingShingle | Fatemeh Manafzadeh Behzad Baradaran Seyed Gholamreza Noor Azar Kamran Javidi Aghdam Reza Dabbaghipour Asghar Shayannia Soudeh Ghafouri-Fard Expression study of Wnt/β-catenin signaling pathway associated lncRNAs in schizophrenia Annals of General Psychiatry Schizophrenia Antipsychotic drugs Wnt/β-catenin pathway Β-catenin lncRNA |
| title | Expression study of Wnt/β-catenin signaling pathway associated lncRNAs in schizophrenia |
| title_full | Expression study of Wnt/β-catenin signaling pathway associated lncRNAs in schizophrenia |
| title_fullStr | Expression study of Wnt/β-catenin signaling pathway associated lncRNAs in schizophrenia |
| title_full_unstemmed | Expression study of Wnt/β-catenin signaling pathway associated lncRNAs in schizophrenia |
| title_short | Expression study of Wnt/β-catenin signaling pathway associated lncRNAs in schizophrenia |
| title_sort | expression study of wnt β catenin signaling pathway associated lncrnas in schizophrenia |
| topic | Schizophrenia Antipsychotic drugs Wnt/β-catenin pathway Β-catenin lncRNA |
| url | https://doi.org/10.1186/s12991-025-00545-1 |
| work_keys_str_mv | AT fatemehmanafzadeh expressionstudyofwntbcateninsignalingpathwayassociatedlncrnasinschizophrenia AT behzadbaradaran expressionstudyofwntbcateninsignalingpathwayassociatedlncrnasinschizophrenia AT seyedgholamrezanoorazar expressionstudyofwntbcateninsignalingpathwayassociatedlncrnasinschizophrenia AT kamranjavidiaghdam expressionstudyofwntbcateninsignalingpathwayassociatedlncrnasinschizophrenia AT rezadabbaghipour expressionstudyofwntbcateninsignalingpathwayassociatedlncrnasinschizophrenia AT asgharshayannia expressionstudyofwntbcateninsignalingpathwayassociatedlncrnasinschizophrenia AT soudehghafourifard expressionstudyofwntbcateninsignalingpathwayassociatedlncrnasinschizophrenia |