Exploring of spectroscopic, dielectric, and bioactivity performance of bioglass/sodium alginate-PVP loaded-Amoxicillin/Clavulanic Acid microspheres for bone tissue engineering
Abstract This study aims to develop an innovative drug delivery bio-system using bioglass (BIOGLASS) and biopolymers of Sodium Alginate (SA) and polyvinylpyrrolidone (PVP) in microsphere form as a carrier for Amoxicillin/Clavulanic Acid drug. In this work BIOGLASS/SA-PVP and Amoxicillin/Clavulanic A...
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Nature Portfolio
2025-05-01
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| Online Access: | https://doi.org/10.1038/s41598-025-96590-7 |
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| author | Ahmed M. Bakr Amany M. El Nahrawy A. M. Mansour Ali B. Abou Hammad |
| author_facet | Ahmed M. Bakr Amany M. El Nahrawy A. M. Mansour Ali B. Abou Hammad |
| author_sort | Ahmed M. Bakr |
| collection | DOAJ |
| description | Abstract This study aims to develop an innovative drug delivery bio-system using bioglass (BIOGLASS) and biopolymers of Sodium Alginate (SA) and polyvinylpyrrolidone (PVP) in microsphere form as a carrier for Amoxicillin/Clavulanic Acid drug. In this work BIOGLASS/SA-PVP and Amoxicillin/Clavulanic Acid loaded BIOGLASS/SA-PVP microspheres (0%, 5%, 10%, and 15%) were synthesized using the ion crosslinking method technique. The fabricated microspheres were analyzed using FT-IR, FESEM/EDX, and XRD confirming the in-vitro examination. XRD and FTIR data demonstrate the effective creation of the apatite layer and the appearance of new apatite peaks at both 605 cm−1 and 565 cm−1, distinguishing the prolonged vibrations associated with the $${\text{PO}}_{4}^{-3}$$ group. SEM images reveal that the prepared bio-beads have a spherical shape, with sizes falling in the micro-scale. The dielectric constant (εʹ), the dielectric loss (εʺ), and the AC conductivity (σ) were slow at the frequency range of 4 Hz to 8 MHz at room temperature. The antibacterial examinations of the fabricated microspheres were performed employing agar diffusion procedure against the clinical pathogens Gram+ and Gram- bacteria. The SBF (simulated body fluid) experiments display the formation of a hydroxy appetite coating on the microsphere’s surfaces that approves their significant bioactivity. Furthermore, antimicrobial results of BIOGLASS/SA-PVP/Amoxicillin/Clavulanic Acid microspheres reveal a notable impact on the antimicrobial performance. The in-vitro tests established that fabricated bio-microspheres are a promising opportunity for bone tissue engineering (substitutes and regeneration), signifying their promise for bone application. |
| format | Article |
| id | doaj-art-b49ff56b955044edb63de9fde7be97fc |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-b49ff56b955044edb63de9fde7be97fc2025-08-20T03:52:19ZengNature PortfolioScientific Reports2045-23222025-05-0115111910.1038/s41598-025-96590-7Exploring of spectroscopic, dielectric, and bioactivity performance of bioglass/sodium alginate-PVP loaded-Amoxicillin/Clavulanic Acid microspheres for bone tissue engineeringAhmed M. Bakr0Amany M. El Nahrawy1A. M. Mansour2Ali B. Abou Hammad3Spectroscopy Department, Physics Research Institute, National Research CentreSolid State Physics Department, Physics Research Institute, National Research CentreSolid State Physics Department, Physics Research Institute, National Research CentreSolid State Physics Department, Physics Research Institute, National Research CentreAbstract This study aims to develop an innovative drug delivery bio-system using bioglass (BIOGLASS) and biopolymers of Sodium Alginate (SA) and polyvinylpyrrolidone (PVP) in microsphere form as a carrier for Amoxicillin/Clavulanic Acid drug. In this work BIOGLASS/SA-PVP and Amoxicillin/Clavulanic Acid loaded BIOGLASS/SA-PVP microspheres (0%, 5%, 10%, and 15%) were synthesized using the ion crosslinking method technique. The fabricated microspheres were analyzed using FT-IR, FESEM/EDX, and XRD confirming the in-vitro examination. XRD and FTIR data demonstrate the effective creation of the apatite layer and the appearance of new apatite peaks at both 605 cm−1 and 565 cm−1, distinguishing the prolonged vibrations associated with the $${\text{PO}}_{4}^{-3}$$ group. SEM images reveal that the prepared bio-beads have a spherical shape, with sizes falling in the micro-scale. The dielectric constant (εʹ), the dielectric loss (εʺ), and the AC conductivity (σ) were slow at the frequency range of 4 Hz to 8 MHz at room temperature. The antibacterial examinations of the fabricated microspheres were performed employing agar diffusion procedure against the clinical pathogens Gram+ and Gram- bacteria. The SBF (simulated body fluid) experiments display the formation of a hydroxy appetite coating on the microsphere’s surfaces that approves their significant bioactivity. Furthermore, antimicrobial results of BIOGLASS/SA-PVP/Amoxicillin/Clavulanic Acid microspheres reveal a notable impact on the antimicrobial performance. The in-vitro tests established that fabricated bio-microspheres are a promising opportunity for bone tissue engineering (substitutes and regeneration), signifying their promise for bone application.https://doi.org/10.1038/s41598-025-96590-7BioglassSodium alginatePVPAmoxicillin/Clavulanic AcidBioactivitySBF |
| spellingShingle | Ahmed M. Bakr Amany M. El Nahrawy A. M. Mansour Ali B. Abou Hammad Exploring of spectroscopic, dielectric, and bioactivity performance of bioglass/sodium alginate-PVP loaded-Amoxicillin/Clavulanic Acid microspheres for bone tissue engineering Scientific Reports Bioglass Sodium alginate PVP Amoxicillin/Clavulanic Acid Bioactivity SBF |
| title | Exploring of spectroscopic, dielectric, and bioactivity performance of bioglass/sodium alginate-PVP loaded-Amoxicillin/Clavulanic Acid microspheres for bone tissue engineering |
| title_full | Exploring of spectroscopic, dielectric, and bioactivity performance of bioglass/sodium alginate-PVP loaded-Amoxicillin/Clavulanic Acid microspheres for bone tissue engineering |
| title_fullStr | Exploring of spectroscopic, dielectric, and bioactivity performance of bioglass/sodium alginate-PVP loaded-Amoxicillin/Clavulanic Acid microspheres for bone tissue engineering |
| title_full_unstemmed | Exploring of spectroscopic, dielectric, and bioactivity performance of bioglass/sodium alginate-PVP loaded-Amoxicillin/Clavulanic Acid microspheres for bone tissue engineering |
| title_short | Exploring of spectroscopic, dielectric, and bioactivity performance of bioglass/sodium alginate-PVP loaded-Amoxicillin/Clavulanic Acid microspheres for bone tissue engineering |
| title_sort | exploring of spectroscopic dielectric and bioactivity performance of bioglass sodium alginate pvp loaded amoxicillin clavulanic acid microspheres for bone tissue engineering |
| topic | Bioglass Sodium alginate PVP Amoxicillin/Clavulanic Acid Bioactivity SBF |
| url | https://doi.org/10.1038/s41598-025-96590-7 |
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