Comparison of Biofilm and Attachment Mechanisms of a Phytopathological and Clinical Isolate of Klebsiella pneumoniae Subsp. pneumoniae
Some bacterial species can colonize humans and plants. It is almost impossible to prevent the contact of clinically pathogenic bacteria with food crops, and if they can persist there, they can reenter the human food chain and cause disease. On the leaf surface, microorganisms are exposed to a number...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/925375 |
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| author | Adriana Marcia Nicolau Korres Gloria Maria de Farias V. Aquije David S. Buss Jose Aires Ventura Patricia Machado Bueno Fernandes Antonio Alberto Ribeiro Fernandes |
| author_facet | Adriana Marcia Nicolau Korres Gloria Maria de Farias V. Aquije David S. Buss Jose Aires Ventura Patricia Machado Bueno Fernandes Antonio Alberto Ribeiro Fernandes |
| author_sort | Adriana Marcia Nicolau Korres |
| collection | DOAJ |
| description | Some bacterial species can colonize humans and plants. It is almost impossible to prevent the contact of clinically pathogenic bacteria with food crops, and if they can persist there, they can reenter the human food chain and cause disease. On the leaf surface, microorganisms are exposed to a number of stress factors. It is unclear how they survive in such different environments. By increasing adhesion to diverse substrates, minimizing environmental differences, and providing protection against defence mechanisms, biofilms could provide part of the answer. Klebsiella pneumoniae subsp. pneumoniae is clinically important and also associated with fruit diseases, such as “pineapple fruit collapse.” We aimed to characterize biofilm formation and adhesion mechanisms of this species isolated from pineapple in comparison with a clinical isolate. No differences were found between the two isolates quantitatively or qualitatively. Both tested positive for capsule formation and were hydrophobic, but neither produced adherence fibres, which might account for their relatively weak adhesion compared to the positive control Staphylococcus epidermidis ATCC 35984. Both produced biofilms on glass and polystyrene, more consistently at 40°C than 35°C, confirmed by atomic force and high-vacuum scanning electron microscopy. Biofilm formation was maintained in an acidic environment, which may be relevant phytopathologically. |
| format | Article |
| id | doaj-art-b49c9142921b47b78f5f09fc1bb1d9d7 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-b49c9142921b47b78f5f09fc1bb1d9d72025-02-03T01:30:05ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/925375925375Comparison of Biofilm and Attachment Mechanisms of a Phytopathological and Clinical Isolate of Klebsiella pneumoniae Subsp. pneumoniaeAdriana Marcia Nicolau Korres0Gloria Maria de Farias V. Aquije1David S. Buss2Jose Aires Ventura3Patricia Machado Bueno Fernandes4Antonio Alberto Ribeiro Fernandes5Núcleo de Biotecnologia, Universidade Federal do Espírito Santo, Avenida Marechal Campos 1468, 29040-090 Vitória, ES, BrazilNúcleo de Biotecnologia, Universidade Federal do Espírito Santo, Avenida Marechal Campos 1468, 29040-090 Vitória, ES, BrazilNúcleo de Biotecnologia, Universidade Federal do Espírito Santo, Avenida Marechal Campos 1468, 29040-090 Vitória, ES, BrazilNúcleo de Biotecnologia, Universidade Federal do Espírito Santo, Avenida Marechal Campos 1468, 29040-090 Vitória, ES, BrazilNúcleo de Biotecnologia, Universidade Federal do Espírito Santo, Avenida Marechal Campos 1468, 29040-090 Vitória, ES, BrazilNúcleo de Biotecnologia, Universidade Federal do Espírito Santo, Avenida Marechal Campos 1468, 29040-090 Vitória, ES, BrazilSome bacterial species can colonize humans and plants. It is almost impossible to prevent the contact of clinically pathogenic bacteria with food crops, and if they can persist there, they can reenter the human food chain and cause disease. On the leaf surface, microorganisms are exposed to a number of stress factors. It is unclear how they survive in such different environments. By increasing adhesion to diverse substrates, minimizing environmental differences, and providing protection against defence mechanisms, biofilms could provide part of the answer. Klebsiella pneumoniae subsp. pneumoniae is clinically important and also associated with fruit diseases, such as “pineapple fruit collapse.” We aimed to characterize biofilm formation and adhesion mechanisms of this species isolated from pineapple in comparison with a clinical isolate. No differences were found between the two isolates quantitatively or qualitatively. Both tested positive for capsule formation and were hydrophobic, but neither produced adherence fibres, which might account for their relatively weak adhesion compared to the positive control Staphylococcus epidermidis ATCC 35984. Both produced biofilms on glass and polystyrene, more consistently at 40°C than 35°C, confirmed by atomic force and high-vacuum scanning electron microscopy. Biofilm formation was maintained in an acidic environment, which may be relevant phytopathologically.http://dx.doi.org/10.1155/2013/925375 |
| spellingShingle | Adriana Marcia Nicolau Korres Gloria Maria de Farias V. Aquije David S. Buss Jose Aires Ventura Patricia Machado Bueno Fernandes Antonio Alberto Ribeiro Fernandes Comparison of Biofilm and Attachment Mechanisms of a Phytopathological and Clinical Isolate of Klebsiella pneumoniae Subsp. pneumoniae The Scientific World Journal |
| title | Comparison of Biofilm and Attachment Mechanisms of a Phytopathological and Clinical Isolate of Klebsiella pneumoniae Subsp. pneumoniae |
| title_full | Comparison of Biofilm and Attachment Mechanisms of a Phytopathological and Clinical Isolate of Klebsiella pneumoniae Subsp. pneumoniae |
| title_fullStr | Comparison of Biofilm and Attachment Mechanisms of a Phytopathological and Clinical Isolate of Klebsiella pneumoniae Subsp. pneumoniae |
| title_full_unstemmed | Comparison of Biofilm and Attachment Mechanisms of a Phytopathological and Clinical Isolate of Klebsiella pneumoniae Subsp. pneumoniae |
| title_short | Comparison of Biofilm and Attachment Mechanisms of a Phytopathological and Clinical Isolate of Klebsiella pneumoniae Subsp. pneumoniae |
| title_sort | comparison of biofilm and attachment mechanisms of a phytopathological and clinical isolate of klebsiella pneumoniae subsp pneumoniae |
| url | http://dx.doi.org/10.1155/2013/925375 |
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